Semicontinuous electrophoresis-convection

The method of electrophoresis-convection for the fractionation of proteins has been modified with the aim of separating quantitatively both electrophoretically slow- and rapid-migrating components, and of obtaining in a single operation pure rapid-moving component. The new technique involves the sup...

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Veröffentlicht in:Archives of biochemistry and biophysics 1953-12, Vol.47 (2), p.455-464
Hauptverfasser: Timasheff, Serge N., Shumaker, John B., Kirkwood, John G.
Format: Artikel
Sprache:eng
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Zusammenfassung:The method of electrophoresis-convection for the fractionation of proteins has been modified with the aim of separating quantitatively both electrophoretically slow- and rapid-migrating components, and of obtaining in a single operation pure rapid-moving component. The new technique involves the superposition of an upward flow of material on the convective circulation in the channel.
ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(53)90482-2