The efficiency of nitrogen fixation of the model legume Medicago truncatula (Jemalong A17) is low compared to Medicago sativa
Medicago truncatula (Gaertn.) (barrel medic) serves as a model legume in plant biology. Numerous studies have addressed molecular aspects of the biology of M. truncatula, while comparatively little is known about the efficiency of N 2 fixation at the whole plant level. The objective of the present s...
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| Veröffentlicht in: | Journal of plant physiology 2010-06, Vol.167 (9), p.683-692 |
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| description | Medicago truncatula (Gaertn.) (barrel medic) serves as a model legume in plant biology. Numerous studies have addressed molecular aspects of the biology of
M. truncatula, while comparatively little is known about the efficiency of N
2 fixation at the whole plant level. The objective of the present study was to compare the efficiency of N
2 fixation of
M. truncatula to the genetically closely related
Medicago sativa (L.) (alfalfa). The relative growth of both species relying exclusively on N
2 fixation versus nitrate nutrition, H
2 evolution, nitrogen assimilation, the concentration of amino acids and organic acids in nodules, and
15N
2 uptake and distribution were studied.
M. truncatula showed much lower efficiency of N
2 fixation. Nodule-specific activity was several-fold lower when compared to
M. sativa, partially as a result of a lower electron allocation to N
2 versus H
+.
M. truncatula or
M. sativa plants grown solely on N
2 fixation as a nitrogen source reached about 30% or 80% of growth, respectively, when compared to plants supplied with sufficient nitrate. Moreover,
M. truncatula had low %N in shoots and a lower allocation of
15N to shoots during 1
h
15N
2 labeling period. Amino acid concentration was about 20% higher in
M. sativa nodules, largely as a result of more asparagine, while the organic acid concentration was about double in
M. sativa, coinciding with a six-fold higher concentration of malate. Total soluble protein in nodules was about three times lower in
M. truncatula and the pattern of enzyme activity in that fraction was strongly different. Sucrose cleaving enzymes displayed higher activity in
M. truncatula nodules, while the activity of phosphoenolpyruvate carboxylase (PEPC) was much lower. It is concluded that the low efficiency of the
M. truncatula symbiotic system is related to a low capacity of organic acid formation and limited nitrogen export from nodules. |
| doi_str_mv | 10.1016/j.jplph.2009.12.016 |
| format | Article |
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M. truncatula, while comparatively little is known about the efficiency of N
2 fixation at the whole plant level. The objective of the present study was to compare the efficiency of N
2 fixation of
M. truncatula to the genetically closely related
Medicago sativa (L.) (alfalfa). The relative growth of both species relying exclusively on N
2 fixation versus nitrate nutrition, H
2 evolution, nitrogen assimilation, the concentration of amino acids and organic acids in nodules, and
15N
2 uptake and distribution were studied.
M. truncatula showed much lower efficiency of N
2 fixation. Nodule-specific activity was several-fold lower when compared to
M. sativa, partially as a result of a lower electron allocation to N
2 versus H
+.
M. truncatula or
M. sativa plants grown solely on N
2 fixation as a nitrogen source reached about 30% or 80% of growth, respectively, when compared to plants supplied with sufficient nitrate. Moreover,
M. truncatula had low %N in shoots and a lower allocation of
15N to shoots during 1
h
15N
2 labeling period. Amino acid concentration was about 20% higher in
M. sativa nodules, largely as a result of more asparagine, while the organic acid concentration was about double in
M. sativa, coinciding with a six-fold higher concentration of malate. Total soluble protein in nodules was about three times lower in
M. truncatula and the pattern of enzyme activity in that fraction was strongly different. Sucrose cleaving enzymes displayed higher activity in
M. truncatula nodules, while the activity of phosphoenolpyruvate carboxylase (PEPC) was much lower. It is concluded that the low efficiency of the
M. truncatula symbiotic system is related to a low capacity of organic acid formation and limited nitrogen export from nodules.</description><identifier>ISSN: 0176-1617</identifier><identifier>EISSN: 1618-1328</identifier><identifier>DOI: 10.1016/j.jplph.2009.12.016</identifier><identifier>PMID: 20207444</identifier><identifier>CODEN: JPPHEY</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>alfalfa ; Allocations ; Amino acids ; Biological and medical sciences ; Biology ; cloves ; enzyme activity ; Fixation ; free amino acids ; Fundamental and applied biological sciences. Psychology ; Legumes ; Medicago sativa ; Medicago sativa - enzymology ; Medicago sativa - metabolism ; Medicago truncatula ; Medicago truncatula - enzymology ; Medicago truncatula - metabolism ; nitrates ; Nitrogen - metabolism ; Nitrogen fixation ; Nitrogen Fixation - physiology ; Nodule ; Nodules ; Organic acids ; organic acids and salts ; Parasitism and symbiosis ; phosphoenolpyruvate carboxylase ; Phosphoenolpyruvate Carboxylase - metabolism ; plant biochemistry ; Plant physiology and development ; Plant Proteins - metabolism ; Plant Shoots - enzymology ; Plant Shoots - metabolism ; Plants (organisms) ; protein content ; root nodules ; sugar content ; Symbiosis</subject><ispartof>Journal of plant physiology, 2010-06, Vol.167 (9), p.683-692</ispartof><rights>2010 Elsevier GmbH</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-36e720e9bae82ccbd5e96511ca32bbdb4cb0f2d20d30cf836471f04d161db5773</citedby><cites>FETCH-LOGICAL-c510t-36e720e9bae82ccbd5e96511ca32bbdb4cb0f2d20d30cf836471f04d161db5773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0176161710000702$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22838744$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20207444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sulieman, Saad</creatorcontrib><creatorcontrib>Schulze, Joachim</creatorcontrib><title>The efficiency of nitrogen fixation of the model legume Medicago truncatula (Jemalong A17) is low compared to Medicago sativa</title><title>Journal of plant physiology</title><addtitle>J Plant Physiol</addtitle><description>Medicago truncatula (Gaertn.) (barrel medic) serves as a model legume in plant biology. Numerous studies have addressed molecular aspects of the biology of
M. truncatula, while comparatively little is known about the efficiency of N
2 fixation at the whole plant level. The objective of the present study was to compare the efficiency of N
2 fixation of
M. truncatula to the genetically closely related
Medicago sativa (L.) (alfalfa). The relative growth of both species relying exclusively on N
2 fixation versus nitrate nutrition, H
2 evolution, nitrogen assimilation, the concentration of amino acids and organic acids in nodules, and
15N
2 uptake and distribution were studied.
M. truncatula showed much lower efficiency of N
2 fixation. Nodule-specific activity was several-fold lower when compared to
M. sativa, partially as a result of a lower electron allocation to N
2 versus H
+.
M. truncatula or
M. sativa plants grown solely on N
2 fixation as a nitrogen source reached about 30% or 80% of growth, respectively, when compared to plants supplied with sufficient nitrate. Moreover,
M. truncatula had low %N in shoots and a lower allocation of
15N to shoots during 1
h
15N
2 labeling period. Amino acid concentration was about 20% higher in
M. sativa nodules, largely as a result of more asparagine, while the organic acid concentration was about double in
M. sativa, coinciding with a six-fold higher concentration of malate. Total soluble protein in nodules was about three times lower in
M. truncatula and the pattern of enzyme activity in that fraction was strongly different. Sucrose cleaving enzymes displayed higher activity in
M. truncatula nodules, while the activity of phosphoenolpyruvate carboxylase (PEPC) was much lower. It is concluded that the low efficiency of the
M. truncatula symbiotic system is related to a low capacity of organic acid formation and limited nitrogen export from nodules.</description><subject>alfalfa</subject><subject>Allocations</subject><subject>Amino acids</subject><subject>Biological and medical sciences</subject><subject>Biology</subject><subject>cloves</subject><subject>enzyme activity</subject><subject>Fixation</subject><subject>free amino acids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Legumes</subject><subject>Medicago sativa</subject><subject>Medicago sativa - enzymology</subject><subject>Medicago sativa - metabolism</subject><subject>Medicago truncatula</subject><subject>Medicago truncatula - enzymology</subject><subject>Medicago truncatula - metabolism</subject><subject>nitrates</subject><subject>Nitrogen - metabolism</subject><subject>Nitrogen fixation</subject><subject>Nitrogen Fixation - physiology</subject><subject>Nodule</subject><subject>Nodules</subject><subject>Organic acids</subject><subject>organic acids and salts</subject><subject>Parasitism and symbiosis</subject><subject>phosphoenolpyruvate carboxylase</subject><subject>Phosphoenolpyruvate Carboxylase - metabolism</subject><subject>plant biochemistry</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Shoots - enzymology</subject><subject>Plant Shoots - metabolism</subject><subject>Plants (organisms)</subject><subject>protein content</subject><subject>root nodules</subject><subject>sugar content</subject><subject>Symbiosis</subject><issn>0176-1617</issn><issn>1618-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1TAURS1ERS-FL0ACTyroIMGPJM4dMKgqnipiQDu2HPs49ZUTBztp6YB_x_cBncHI8tbaR8deCL2gpKSENm835Wby003JCFmXlJU5e4RWtKFtQTlrH6MVoaIpciCO0dOUNiTf65Y_QceMMCKqqlqhX1c3gMFapx2M-h4Hi0c3x9DDiK37qWYXxm04Z2wIBjz20C8D4K9gnFZ9wHNcRq3mxSv85gsMyoexx-dUnGGXsA93WIdhUhEMnsNDK-XJt-oZOrLKJ3h-OE_Q9Yf3VxefistvHz9fnF8WuqZkLngDghFYdwpapnVnalg3NaVacdZ1pqt0RywzjBhOtG15UwlqSWXy001XC8FP0Ov93CmGHwukWQ4uafBejRCWJEXNRU0rUf2f5LxeU1LxTPI9qWNIKYKVU3SDiveSErkVJDdyJ0huBUnKZM5y6-Vh_tINYP52_hjJwOkBUEkrb6MatUsPHGt5K3bcqz1nVZCqj5m5_s4I5YS2nOQtM_FuT0D-2VsHUaad5Kwggp6lCe6fq_4GT0W46Q</recordid><startdate>20100615</startdate><enddate>20100615</enddate><creator>Sulieman, Saad</creator><creator>Schulze, Joachim</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20100615</creationdate><title>The efficiency of nitrogen fixation of the model legume Medicago truncatula (Jemalong A17) is low compared to Medicago sativa</title><author>Sulieman, Saad ; Schulze, Joachim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-36e720e9bae82ccbd5e96511ca32bbdb4cb0f2d20d30cf836471f04d161db5773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>alfalfa</topic><topic>Allocations</topic><topic>Amino acids</topic><topic>Biological and medical sciences</topic><topic>Biology</topic><topic>cloves</topic><topic>enzyme activity</topic><topic>Fixation</topic><topic>free amino acids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Legumes</topic><topic>Medicago sativa</topic><topic>Medicago sativa - enzymology</topic><topic>Medicago sativa - metabolism</topic><topic>Medicago truncatula</topic><topic>Medicago truncatula - enzymology</topic><topic>Medicago truncatula - metabolism</topic><topic>nitrates</topic><topic>Nitrogen - metabolism</topic><topic>Nitrogen fixation</topic><topic>Nitrogen Fixation - physiology</topic><topic>Nodule</topic><topic>Nodules</topic><topic>Organic acids</topic><topic>organic acids and salts</topic><topic>Parasitism and symbiosis</topic><topic>phosphoenolpyruvate carboxylase</topic><topic>Phosphoenolpyruvate Carboxylase - metabolism</topic><topic>plant biochemistry</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Shoots - enzymology</topic><topic>Plant Shoots - metabolism</topic><topic>Plants (organisms)</topic><topic>protein content</topic><topic>root nodules</topic><topic>sugar content</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sulieman, Saad</creatorcontrib><creatorcontrib>Schulze, Joachim</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sulieman, Saad</au><au>Schulze, Joachim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The efficiency of nitrogen fixation of the model legume Medicago truncatula (Jemalong A17) is low compared to Medicago sativa</atitle><jtitle>Journal of plant physiology</jtitle><addtitle>J Plant Physiol</addtitle><date>2010-06-15</date><risdate>2010</risdate><volume>167</volume><issue>9</issue><spage>683</spage><epage>692</epage><pages>683-692</pages><issn>0176-1617</issn><eissn>1618-1328</eissn><coden>JPPHEY</coden><abstract>Medicago truncatula (Gaertn.) (barrel medic) serves as a model legume in plant biology. Numerous studies have addressed molecular aspects of the biology of
M. truncatula, while comparatively little is known about the efficiency of N
2 fixation at the whole plant level. The objective of the present study was to compare the efficiency of N
2 fixation of
M. truncatula to the genetically closely related
Medicago sativa (L.) (alfalfa). The relative growth of both species relying exclusively on N
2 fixation versus nitrate nutrition, H
2 evolution, nitrogen assimilation, the concentration of amino acids and organic acids in nodules, and
15N
2 uptake and distribution were studied.
M. truncatula showed much lower efficiency of N
2 fixation. Nodule-specific activity was several-fold lower when compared to
M. sativa, partially as a result of a lower electron allocation to N
2 versus H
+.
M. truncatula or
M. sativa plants grown solely on N
2 fixation as a nitrogen source reached about 30% or 80% of growth, respectively, when compared to plants supplied with sufficient nitrate. Moreover,
M. truncatula had low %N in shoots and a lower allocation of
15N to shoots during 1
h
15N
2 labeling period. Amino acid concentration was about 20% higher in
M. sativa nodules, largely as a result of more asparagine, while the organic acid concentration was about double in
M. sativa, coinciding with a six-fold higher concentration of malate. Total soluble protein in nodules was about three times lower in
M. truncatula and the pattern of enzyme activity in that fraction was strongly different. Sucrose cleaving enzymes displayed higher activity in
M. truncatula nodules, while the activity of phosphoenolpyruvate carboxylase (PEPC) was much lower. It is concluded that the low efficiency of the
M. truncatula symbiotic system is related to a low capacity of organic acid formation and limited nitrogen export from nodules.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>20207444</pmid><doi>10.1016/j.jplph.2009.12.016</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of plant physiology, 2010-06, Vol.167 (9), p.683-692 |
| issn | 0176-1617 1618-1328 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | alfalfa Allocations Amino acids Biological and medical sciences Biology cloves enzyme activity Fixation free amino acids Fundamental and applied biological sciences. Psychology Legumes Medicago sativa Medicago sativa - enzymology Medicago sativa - metabolism Medicago truncatula Medicago truncatula - enzymology Medicago truncatula - metabolism nitrates Nitrogen - metabolism Nitrogen fixation Nitrogen Fixation - physiology Nodule Nodules Organic acids organic acids and salts Parasitism and symbiosis phosphoenolpyruvate carboxylase Phosphoenolpyruvate Carboxylase - metabolism plant biochemistry Plant physiology and development Plant Proteins - metabolism Plant Shoots - enzymology Plant Shoots - metabolism Plants (organisms) protein content root nodules sugar content Symbiosis |
| title | The efficiency of nitrogen fixation of the model legume Medicago truncatula (Jemalong A17) is low compared to Medicago sativa |
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