Impact of caspase activation in human spermatozoa
Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controvers...
Gespeichert in:
| Veröffentlicht in: | Microscopy research and technique 2009-11, Vol.72 (11), p.878-888 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 888 |
|---|---|
| container_issue | 11 |
| container_start_page | 878 |
| container_title | Microscopy research and technique |
| container_volume | 72 |
| creator | Grunewald, Sonja Sharma, Rakesh Paasch, Uwe Glander, Hans-Juergen Agarwal, Ashok |
| description | Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controversially discussed in recent years, many studies demonstrated their activation in male germ cells. Activated apoptosis signaling results in decreased fertilizing capacity of the sperm. This review presents the current knowledge on the role of caspases in human sperm. Techniques of caspase monitoring are highlighted. With regard to the high impact of caspases on the sperm fertilizing potential, physiological and pathological settings of caspase activation and inactivation are discussed. Finally, the effects of depletion of caspase‐positive sperm are shown with various standard and molecular sperm preparation methods. Microsc. Res. Tech., 2009. © 2009 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jemt.20732 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_753693805</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>753693805</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4342-6101eb23bcb844879e41c8c08b7516bb942b21a8e3c7167bbb4bf02fe74ec4293</originalsourceid><addsrcrecordid>eNqFkM1LwzAchoMobk4v_gHSmyB05pekTXMUmXNjfiCTDS8hiSl2tmttWnX-9XZ26k1Pvw-e9zm8CB0C7gPG5HRhs6pPMKdkC3UBC-43X7G93gPhC8DzDtpzboExQABsF3VAsCAII9ZFMMoKZSovjz2jXKGc9ZozeVVVki-9ZOk91Zlaeq6wZaaq_CNX-2gnVqmzB5vZQ_cXg-n5pT-5GY7Ozya-YZQRPwQMVhOqjY4Yi7iwDExkcKR5AKHWghFNQEWWGg4h11ozHWMSW86sYUTQHjpuvUWZv9TWVTJLnLFpqpY2r53kAQ0FjXDwP0kZgMCcNeRJS5oyd660sSzKJFPlSgKW6y7lukv51WUDH220tc7s4y-6Ka8BoAXektSu_lDJ8eBq-i3120ziKvv-k1Hlsww55YGcXQ_lw_wunEXTWzmmnzzEjMs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734119074</pqid></control><display><type>article</type><title>Impact of caspase activation in human spermatozoa</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Grunewald, Sonja ; Sharma, Rakesh ; Paasch, Uwe ; Glander, Hans-Juergen ; Agarwal, Ashok</creator><creatorcontrib>Grunewald, Sonja ; Sharma, Rakesh ; Paasch, Uwe ; Glander, Hans-Juergen ; Agarwal, Ashok</creatorcontrib><description>Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controversially discussed in recent years, many studies demonstrated their activation in male germ cells. Activated apoptosis signaling results in decreased fertilizing capacity of the sperm. This review presents the current knowledge on the role of caspases in human sperm. Techniques of caspase monitoring are highlighted. With regard to the high impact of caspases on the sperm fertilizing potential, physiological and pathological settings of caspase activation and inactivation are discussed. Finally, the effects of depletion of caspase‐positive sperm are shown with various standard and molecular sperm preparation methods. Microsc. Res. Tech., 2009. © 2009 Wiley‐Liss, Inc.</description><identifier>ISSN: 1059-910X</identifier><identifier>EISSN: 1097-0029</identifier><identifier>DOI: 10.1002/jemt.20732</identifier><identifier>PMID: 19455684</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Activation ; Apoptosis ; Cascades ; caspase assays ; Caspases - biosynthesis ; Cell Survival ; Cellular ; Cryopreservation ; Depletion ; Fertilizing ; Human ; Humans ; Male ; sperm cryopreservation ; sperm maturation ; sperm separation ; Spermatozoa ; Spermatozoa - enzymology ; Spermatozoa - physiology</subject><ispartof>Microscopy research and technique, 2009-11, Vol.72 (11), p.878-888</ispartof><rights>Copyright © 2009 Wiley‐Liss, Inc.</rights><rights>(c) 2009 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4342-6101eb23bcb844879e41c8c08b7516bb942b21a8e3c7167bbb4bf02fe74ec4293</citedby><cites>FETCH-LOGICAL-c4342-6101eb23bcb844879e41c8c08b7516bb942b21a8e3c7167bbb4bf02fe74ec4293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjemt.20732$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjemt.20732$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19455684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grunewald, Sonja</creatorcontrib><creatorcontrib>Sharma, Rakesh</creatorcontrib><creatorcontrib>Paasch, Uwe</creatorcontrib><creatorcontrib>Glander, Hans-Juergen</creatorcontrib><creatorcontrib>Agarwal, Ashok</creatorcontrib><title>Impact of caspase activation in human spermatozoa</title><title>Microscopy research and technique</title><addtitle>Microsc. Res. Tech</addtitle><description>Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controversially discussed in recent years, many studies demonstrated their activation in male germ cells. Activated apoptosis signaling results in decreased fertilizing capacity of the sperm. This review presents the current knowledge on the role of caspases in human sperm. Techniques of caspase monitoring are highlighted. With regard to the high impact of caspases on the sperm fertilizing potential, physiological and pathological settings of caspase activation and inactivation are discussed. Finally, the effects of depletion of caspase‐positive sperm are shown with various standard and molecular sperm preparation methods. Microsc. Res. Tech., 2009. © 2009 Wiley‐Liss, Inc.</description><subject>Activation</subject><subject>Apoptosis</subject><subject>Cascades</subject><subject>caspase assays</subject><subject>Caspases - biosynthesis</subject><subject>Cell Survival</subject><subject>Cellular</subject><subject>Cryopreservation</subject><subject>Depletion</subject><subject>Fertilizing</subject><subject>Human</subject><subject>Humans</subject><subject>Male</subject><subject>sperm cryopreservation</subject><subject>sperm maturation</subject><subject>sperm separation</subject><subject>Spermatozoa</subject><subject>Spermatozoa - enzymology</subject><subject>Spermatozoa - physiology</subject><issn>1059-910X</issn><issn>1097-0029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1LwzAchoMobk4v_gHSmyB05pekTXMUmXNjfiCTDS8hiSl2tmttWnX-9XZ26k1Pvw-e9zm8CB0C7gPG5HRhs6pPMKdkC3UBC-43X7G93gPhC8DzDtpzboExQABsF3VAsCAII9ZFMMoKZSovjz2jXKGc9ZozeVVVki-9ZOk91Zlaeq6wZaaq_CNX-2gnVqmzB5vZQ_cXg-n5pT-5GY7Ozya-YZQRPwQMVhOqjY4Yi7iwDExkcKR5AKHWghFNQEWWGg4h11ozHWMSW86sYUTQHjpuvUWZv9TWVTJLnLFpqpY2r53kAQ0FjXDwP0kZgMCcNeRJS5oyd660sSzKJFPlSgKW6y7lukv51WUDH220tc7s4y-6Ka8BoAXektSu_lDJ8eBq-i3120ziKvv-k1Hlsww55YGcXQ_lw_wunEXTWzmmnzzEjMs</recordid><startdate>200911</startdate><enddate>200911</enddate><creator>Grunewald, Sonja</creator><creator>Sharma, Rakesh</creator><creator>Paasch, Uwe</creator><creator>Glander, Hans-Juergen</creator><creator>Agarwal, Ashok</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>200911</creationdate><title>Impact of caspase activation in human spermatozoa</title><author>Grunewald, Sonja ; Sharma, Rakesh ; Paasch, Uwe ; Glander, Hans-Juergen ; Agarwal, Ashok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4342-6101eb23bcb844879e41c8c08b7516bb942b21a8e3c7167bbb4bf02fe74ec4293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Activation</topic><topic>Apoptosis</topic><topic>Cascades</topic><topic>caspase assays</topic><topic>Caspases - biosynthesis</topic><topic>Cell Survival</topic><topic>Cellular</topic><topic>Cryopreservation</topic><topic>Depletion</topic><topic>Fertilizing</topic><topic>Human</topic><topic>Humans</topic><topic>Male</topic><topic>sperm cryopreservation</topic><topic>sperm maturation</topic><topic>sperm separation</topic><topic>Spermatozoa</topic><topic>Spermatozoa - enzymology</topic><topic>Spermatozoa - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grunewald, Sonja</creatorcontrib><creatorcontrib>Sharma, Rakesh</creatorcontrib><creatorcontrib>Paasch, Uwe</creatorcontrib><creatorcontrib>Glander, Hans-Juergen</creatorcontrib><creatorcontrib>Agarwal, Ashok</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Microscopy research and technique</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grunewald, Sonja</au><au>Sharma, Rakesh</au><au>Paasch, Uwe</au><au>Glander, Hans-Juergen</au><au>Agarwal, Ashok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of caspase activation in human spermatozoa</atitle><jtitle>Microscopy research and technique</jtitle><addtitle>Microsc. Res. Tech</addtitle><date>2009-11</date><risdate>2009</risdate><volume>72</volume><issue>11</issue><spage>878</spage><epage>888</epage><pages>878-888</pages><issn>1059-910X</issn><eissn>1097-0029</eissn><abstract>Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controversially discussed in recent years, many studies demonstrated their activation in male germ cells. Activated apoptosis signaling results in decreased fertilizing capacity of the sperm. This review presents the current knowledge on the role of caspases in human sperm. Techniques of caspase monitoring are highlighted. With regard to the high impact of caspases on the sperm fertilizing potential, physiological and pathological settings of caspase activation and inactivation are discussed. Finally, the effects of depletion of caspase‐positive sperm are shown with various standard and molecular sperm preparation methods. Microsc. Res. Tech., 2009. © 2009 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>19455684</pmid><doi>10.1002/jemt.20732</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1059-910X |
| ispartof | Microscopy research and technique, 2009-11, Vol.72 (11), p.878-888 |
| issn | 1059-910X 1097-0029 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_753693805 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Activation Apoptosis Cascades caspase assays Caspases - biosynthesis Cell Survival Cellular Cryopreservation Depletion Fertilizing Human Humans Male sperm cryopreservation sperm maturation sperm separation Spermatozoa Spermatozoa - enzymology Spermatozoa - physiology |
| title | Impact of caspase activation in human spermatozoa |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T00%3A28%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impact%20of%20caspase%20activation%20in%20human%20spermatozoa&rft.jtitle=Microscopy%20research%20and%20technique&rft.au=Grunewald,%20Sonja&rft.date=2009-11&rft.volume=72&rft.issue=11&rft.spage=878&rft.epage=888&rft.pages=878-888&rft.issn=1059-910X&rft.eissn=1097-0029&rft_id=info:doi/10.1002/jemt.20732&rft_dat=%3Cproquest_cross%3E753693805%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=734119074&rft_id=info:pmid/19455684&rfr_iscdi=true |