Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography
This paper describes how distribution ratios were used for prediction of peak elution in analytical high-performance counter-current chromatography (HPCCC) to explore the method for separation and purification of bioactive compounds from the roots of Menispermum dauricum. Then important parameters r...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-07, Vol.878 (22), p.1929-1933 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Luo, Houding Peng, Ming Ye, Haoyu Chen, Lijuan Peng, Aihua Tang, Minghai Zhang, Fan Shi, Jie |
| description | This paper describes how distribution ratios were used for prediction of peak elution in analytical high-performance counter-current chromatography (HPCCC) to explore the method for separation and purification of bioactive compounds from the roots of
Menispermum dauricum. Then important parameters related to HPCCC separations including solvent systems, sample concentration, sample loading volume and flow rate were optimized on an analytical Mini-DE HPCCC and finally linearly scaled up to a preparative Midi-DE HPCCC with nearly the same resolutions and separation time. Four phenolic alkaloids were for the first time obtained by HPCCC separation with a two-phase solvent system composed of petroleum ether–ethyl acetate–ethanol–water (1:2:1:2, v/v). This process produced 131.3
mg daurisolin, 197.1
mg dauricine, 32.4
mg daurinoline and 14.7
mg dauricicoline with the purity of 97.6%, 96.4%, 97.2% and 98.3%, respectively from 500
mg crude extract of the roots of
M. dauricum in a one-step separation. The purities of compounds were determined by high-performance liquid chromatography (HPLC). Their structures were identified by electrospray ionization mass spectrometer (ESI-MS) and nuclear magnetic resonance (NMR). |
| doi_str_mv | 10.1016/j.jchromb.2010.05.002 |
| format | Article |
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Menispermum dauricum. Then important parameters related to HPCCC separations including solvent systems, sample concentration, sample loading volume and flow rate were optimized on an analytical Mini-DE HPCCC and finally linearly scaled up to a preparative Midi-DE HPCCC with nearly the same resolutions and separation time. Four phenolic alkaloids were for the first time obtained by HPCCC separation with a two-phase solvent system composed of petroleum ether–ethyl acetate–ethanol–water (1:2:1:2, v/v). This process produced 131.3
mg daurisolin, 197.1
mg dauricine, 32.4
mg daurinoline and 14.7
mg dauricicoline with the purity of 97.6%, 96.4%, 97.2% and 98.3%, respectively from 500
mg crude extract of the roots of
M. dauricum in a one-step separation. The purities of compounds were determined by high-performance liquid chromatography (HPLC). Their structures were identified by electrospray ionization mass spectrometer (ESI-MS) and nuclear magnetic resonance (NMR).</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2010.05.002</identifier><identifier>PMID: 20576475</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Alkaloids ; Alkaloids - analysis ; Alkaloids - isolation & purification ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography ; Countercurrent Distribution - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; HPCCC ; Linear scale-up ; Mathematical analysis ; Medical sciences ; Menispermum - chemistry ; Menispermum dauricum ; Nuclear magnetic resonance ; Pharmacology. Drug treatments ; Phenolic alkaloids ; Plant Extracts - analysis ; Plant Extracts - isolation & purification ; Plant Roots - chemistry ; Purity ; Roots ; Separation ; Solvents</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2010-07, Vol.878 (22), p.1929-1933</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>2010. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-11797f8ab14c851c77ef778563ad59bee51e3f7b47aa8ebca64a2675b71156663</citedby><cites>FETCH-LOGICAL-c525t-11797f8ab14c851c77ef778563ad59bee51e3f7b47aa8ebca64a2675b71156663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2010.05.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22989998$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20576475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Houding</creatorcontrib><creatorcontrib>Peng, Ming</creatorcontrib><creatorcontrib>Ye, Haoyu</creatorcontrib><creatorcontrib>Chen, Lijuan</creatorcontrib><creatorcontrib>Peng, Aihua</creatorcontrib><creatorcontrib>Tang, Minghai</creatorcontrib><creatorcontrib>Zhang, Fan</creatorcontrib><creatorcontrib>Shi, Jie</creatorcontrib><title>Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>This paper describes how distribution ratios were used for prediction of peak elution in analytical high-performance counter-current chromatography (HPCCC) to explore the method for separation and purification of bioactive compounds from the roots of
Menispermum dauricum. Then important parameters related to HPCCC separations including solvent systems, sample concentration, sample loading volume and flow rate were optimized on an analytical Mini-DE HPCCC and finally linearly scaled up to a preparative Midi-DE HPCCC with nearly the same resolutions and separation time. Four phenolic alkaloids were for the first time obtained by HPCCC separation with a two-phase solvent system composed of petroleum ether–ethyl acetate–ethanol–water (1:2:1:2, v/v). This process produced 131.3
mg daurisolin, 197.1
mg dauricine, 32.4
mg daurinoline and 14.7
mg dauricicoline with the purity of 97.6%, 96.4%, 97.2% and 98.3%, respectively from 500
mg crude extract of the roots of
M. dauricum in a one-step separation. The purities of compounds were determined by high-performance liquid chromatography (HPLC). Their structures were identified by electrospray ionization mass spectrometer (ESI-MS) and nuclear magnetic resonance (NMR).</description><subject>Alkaloids</subject><subject>Alkaloids - analysis</subject><subject>Alkaloids - isolation & purification</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography</subject><subject>Countercurrent Distribution - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>HPCCC</subject><subject>Linear scale-up</subject><subject>Mathematical analysis</subject><subject>Medical sciences</subject><subject>Menispermum - chemistry</subject><subject>Menispermum dauricum</subject><subject>Nuclear magnetic resonance</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenolic alkaloids</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Roots - chemistry</subject><subject>Purity</subject><subject>Roots</subject><subject>Separation</subject><subject>Solvents</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-O1SAUhxujccbRR9CwMa56hVKgXRkz8V8yRheauCOn9PSWa1vqgZrM6_ikcr1XXc6KE_gOHH5fUTwVfCe40C8Pu4MbKczdruJ5j6sd59W94lI0RpbS6G_3c60ML3klq4viUYwHzoXhRj4sLiqujK6Nuix-fSbsvUvQTchg6dnkFwRi0cGE5bayMLAhbMTWEZcwecdg-g5T8H1kEVcgSD4sbMiTsDQioxBSPDZ9xMXHFWneZtbDRt7lYot-2bPR78cyHw2BZlgcMhe2JSGVbiPCJbE_H4MU9gTrePu4eDDAFPHJeb0qvr598-X6fXnz6d2H69c3pVOVSqUQpjVDA52oXaOEMwYHYxqlJfSq7RCVQDmYrjYADXYOdA2VNqozQiittbwqXpzuXSn82DAmO_vocJpgwbBFa5TUjaxNdTcppa5Fq-tMqhPpKMRIONiV_Ax0awW3R4_2YM8e7dGj5cpmj7nv2fmFrZux_9f1V1wGnp8BOKoaKAfp43-uapu2bZvMvTpxmJP76ZFsdB5z6L0ndMn2wd8xym-Z98KY</recordid><startdate>20100715</startdate><enddate>20100715</enddate><creator>Luo, Houding</creator><creator>Peng, Ming</creator><creator>Ye, Haoyu</creator><creator>Chen, Lijuan</creator><creator>Peng, Aihua</creator><creator>Tang, Minghai</creator><creator>Zhang, Fan</creator><creator>Shi, Jie</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope></search><sort><creationdate>20100715</creationdate><title>Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography</title><author>Luo, Houding ; Peng, Ming ; Ye, Haoyu ; Chen, Lijuan ; Peng, Aihua ; Tang, Minghai ; Zhang, Fan ; Shi, Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-11797f8ab14c851c77ef778563ad59bee51e3f7b47aa8ebca64a2675b71156663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Alkaloids</topic><topic>Alkaloids - analysis</topic><topic>Alkaloids - isolation & purification</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography</topic><topic>Countercurrent Distribution - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>HPCCC</topic><topic>Linear scale-up</topic><topic>Mathematical analysis</topic><topic>Medical sciences</topic><topic>Menispermum - chemistry</topic><topic>Menispermum dauricum</topic><topic>Nuclear magnetic resonance</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenolic alkaloids</topic><topic>Plant Extracts - analysis</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Roots - chemistry</topic><topic>Purity</topic><topic>Roots</topic><topic>Separation</topic><topic>Solvents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Houding</creatorcontrib><creatorcontrib>Peng, Ming</creatorcontrib><creatorcontrib>Ye, Haoyu</creatorcontrib><creatorcontrib>Chen, Lijuan</creatorcontrib><creatorcontrib>Peng, Aihua</creatorcontrib><creatorcontrib>Tang, Minghai</creatorcontrib><creatorcontrib>Zhang, Fan</creatorcontrib><creatorcontrib>Shi, Jie</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Houding</au><au>Peng, Ming</au><au>Ye, Haoyu</au><au>Chen, Lijuan</au><au>Peng, Aihua</au><au>Tang, Minghai</au><au>Zhang, Fan</au><au>Shi, Jie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2010-07-15</date><risdate>2010</risdate><volume>878</volume><issue>22</issue><spage>1929</spage><epage>1933</epage><pages>1929-1933</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>This paper describes how distribution ratios were used for prediction of peak elution in analytical high-performance counter-current chromatography (HPCCC) to explore the method for separation and purification of bioactive compounds from the roots of
Menispermum dauricum. Then important parameters related to HPCCC separations including solvent systems, sample concentration, sample loading volume and flow rate were optimized on an analytical Mini-DE HPCCC and finally linearly scaled up to a preparative Midi-DE HPCCC with nearly the same resolutions and separation time. Four phenolic alkaloids were for the first time obtained by HPCCC separation with a two-phase solvent system composed of petroleum ether–ethyl acetate–ethanol–water (1:2:1:2, v/v). This process produced 131.3
mg daurisolin, 197.1
mg dauricine, 32.4
mg daurinoline and 14.7
mg dauricicoline with the purity of 97.6%, 96.4%, 97.2% and 98.3%, respectively from 500
mg crude extract of the roots of
M. dauricum in a one-step separation. The purities of compounds were determined by high-performance liquid chromatography (HPLC). Their structures were identified by electrospray ionization mass spectrometer (ESI-MS) and nuclear magnetic resonance (NMR).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20576475</pmid><doi>10.1016/j.jchromb.2010.05.002</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2010-07, Vol.878 (22), p.1929-1933 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_753683472 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Alkaloids Alkaloids - analysis Alkaloids - isolation & purification Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography Countercurrent Distribution - methods Fundamental and applied biological sciences. Psychology General pharmacology HPCCC Linear scale-up Mathematical analysis Medical sciences Menispermum - chemistry Menispermum dauricum Nuclear magnetic resonance Pharmacology. Drug treatments Phenolic alkaloids Plant Extracts - analysis Plant Extracts - isolation & purification Plant Roots - chemistry Purity Roots Separation Solvents |
| title | Predictable and linear scale-up of four phenolic alkaloids separation from the roots of Menispermum dauricum using high-performance counter-current chromatography |
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