Quantification of UV-induced cyclobutane pyrimidine dimers using an oligonucleotide chip assay

A lesion-specific enzyme-induced DNA strand break assay was developed for an oligonucleotide chip for the determination of UVB-induced cyclobutane pyrimidine dimers (CPDs). A 20-mer of fluorophore-labeled and biotinylated oligonucleotide was immobilized on the chip. CPDs in DNA on the chip were form...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2010-07, Vol.397 (6), p.2271-2277
Hauptverfasser:	Kim, Min Jung, Lee, Su Chul, Kang, Seong Ho, Choo, Jaebum, Song, Joon Myong
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Assaying Biochemistry Biotin Calibration Carbocyclic compounds Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chips Cyclobutane Cyclobutane pyrimidine dimer Deoxyribonuclease (Pyrimidine Dimer) - metabolism Detectors Dimers DNA DNA Breaks - radiation effects DNA Damage - genetics Enzymes Exact sciences and technology Fluorescence Fluorescent Dyes Food Science Laboratory Medicine Lesion-specific enzyme-induced break assay Mathematical analysis Monitoring/Environmental Analysis Oligonucleotide Array Sequence Analysis - methods Oligonucleotide Array Sequence Analysis - standards Oligonucleotide chip Oligonucleotides Pyrimidine Dimers - analysis Pyrimidine Dimers - radiation effects Pyrimidines Short Communication Spectrometric and optical methods T4 endonuclease V Ultraviolet Rays - adverse effects Viral Proteins - metabolism
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