Determination of atorvastatin and its metabolite ortho-hydroxyatorvastatin in human plasma by on-line anion-exchange solid-phase extraction and liquid chromatography tandem mass spectrometry
A rapid, sensitive, and specific method was developed and validated using liquid chromatography-tandem mass spectrometry for the simultaneous quantitation of atorvastatin (ATV) and its major metabolite ortho-hydroxyatorvastatin (o-HATV) in human plasma. The sample preparation involved a liquid-liqui...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2009-07, Vol.394 (6), p.1687-1696 |
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| creator | Guillén, David Cofán, Frederic Ros, Emilio Millán, Olga Cofán, Montse Rimola, Antoni Brunet, Mercè |
| description | A rapid, sensitive, and specific method was developed and validated using liquid chromatography-tandem mass spectrometry for the simultaneous quantitation of atorvastatin (ATV) and its major metabolite ortho-hydroxyatorvastatin (o-HATV) in human plasma. The sample preparation involved a liquid-liquid extraction without chlorinated solvents and an on-line solid-phase extraction exploring the possibilities that anion exchange offers. The analytical method presented intraday and day-to-day variation below 10%; intraday and day-to-day accuracy stood between 94% and 105%; the limit of quantification was 0.1 ng/mL for ATV and 0.5 ng/mL for o-HATV; and the recovery was above 75% for both molecules. This method was applied successfully to quantitate ATV and o-HATV concentrations in an unstudied renal transplant recipient cohort treated with an immunosuppressive regime of tacrolimus and mycophenolic acid and a cohort of hypercholesterolemic patients included in the study as a control group. It can be used to evaluate patient adherence, drug-drug interactions, and pharmacokinetic/pharmacodynamic relationships. The results in our study showed that ATV and o-HATV levels in the renal transplant group were significantly increased (p < 0.001), compared to the hypercholesterolemic group. |
| doi_str_mv | 10.1007/s00216-009-2852-3 |
| format | Article |
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The sample preparation involved a liquid-liquid extraction without chlorinated solvents and an on-line solid-phase extraction exploring the possibilities that anion exchange offers. The analytical method presented intraday and day-to-day variation below 10%; intraday and day-to-day accuracy stood between 94% and 105%; the limit of quantification was 0.1 ng/mL for ATV and 0.5 ng/mL for o-HATV; and the recovery was above 75% for both molecules. This method was applied successfully to quantitate ATV and o-HATV concentrations in an unstudied renal transplant recipient cohort treated with an immunosuppressive regime of tacrolimus and mycophenolic acid and a cohort of hypercholesterolemic patients included in the study as a control group. It can be used to evaluate patient adherence, drug-drug interactions, and pharmacokinetic/pharmacodynamic relationships. The results in our study showed that ATV and o-HATV levels in the renal transplant group were significantly increased (p < 0.001), compared to the hypercholesterolemic group.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-009-2852-3</identifier><identifier>PMID: 19506841</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Adult ; Aged ; All terrain vehicles ; Analytical Chemistry ; Anions - chemistry ; Atorvastatin Calcium ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, Liquid - methods ; Exact sciences and technology ; Extraction ; Female ; Food Science ; Heptanoic Acids - blood ; Heptanoic Acids - chemistry ; Heptanoic Acids - metabolism ; Human ; Humans ; Laboratory Medicine ; Male ; Mass spectrometry ; Mathematical analysis ; Metabolites ; Middle Aged ; Monitoring/Environmental Analysis ; On-line systems ; Online Systems ; Original Paper ; Other chromatographic methods ; Pyrroles - blood ; Pyrroles - chemistry ; Pyrroles - metabolism ; Solid Phase Extraction - methods ; Spectrometric and optical methods ; Tandem Mass Spectrometry - methods ; Transplants</subject><ispartof>Analytical and bioanalytical chemistry, 2009-07, Vol.394 (6), p.1687-1696</ispartof><rights>Springer-Verlag 2009</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-8d5af558ab8023edfa35a4ef499a9a3cac4ba7265d8e8b1ab94129530d793ae53</citedby><cites>FETCH-LOGICAL-c461t-8d5af558ab8023edfa35a4ef499a9a3cac4ba7265d8e8b1ab94129530d793ae53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-009-2852-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-009-2852-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21661552$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19506841$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guillén, David</creatorcontrib><creatorcontrib>Cofán, Frederic</creatorcontrib><creatorcontrib>Ros, Emilio</creatorcontrib><creatorcontrib>Millán, Olga</creatorcontrib><creatorcontrib>Cofán, Montse</creatorcontrib><creatorcontrib>Rimola, Antoni</creatorcontrib><creatorcontrib>Brunet, Mercè</creatorcontrib><title>Determination of atorvastatin and its metabolite ortho-hydroxyatorvastatin in human plasma by on-line anion-exchange solid-phase extraction and liquid chromatography tandem mass spectrometry</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>A rapid, sensitive, and specific method was developed and validated using liquid chromatography-tandem mass spectrometry for the simultaneous quantitation of atorvastatin (ATV) and its major metabolite ortho-hydroxyatorvastatin (o-HATV) in human plasma. 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Cofán, Frederic ; Ros, Emilio ; Millán, Olga ; Cofán, Montse ; Rimola, Antoni ; Brunet, Mercè</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-8d5af558ab8023edfa35a4ef499a9a3cac4ba7265d8e8b1ab94129530d793ae53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Aged</topic><topic>All terrain vehicles</topic><topic>Analytical Chemistry</topic><topic>Anions - chemistry</topic><topic>Atorvastatin Calcium</topic><topic>Biochemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, Liquid - methods</topic><topic>Exact sciences and technology</topic><topic>Extraction</topic><topic>Female</topic><topic>Food Science</topic><topic>Heptanoic Acids - blood</topic><topic>Heptanoic Acids - chemistry</topic><topic>Heptanoic Acids - metabolism</topic><topic>Human</topic><topic>Humans</topic><topic>Laboratory Medicine</topic><topic>Male</topic><topic>Mass spectrometry</topic><topic>Mathematical analysis</topic><topic>Metabolites</topic><topic>Middle Aged</topic><topic>Monitoring/Environmental Analysis</topic><topic>On-line systems</topic><topic>Online Systems</topic><topic>Original Paper</topic><topic>Other chromatographic methods</topic><topic>Pyrroles - blood</topic><topic>Pyrroles - chemistry</topic><topic>Pyrroles - metabolism</topic><topic>Solid Phase Extraction - methods</topic><topic>Spectrometric and optical methods</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Transplants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guillén, David</creatorcontrib><creatorcontrib>Cofán, Frederic</creatorcontrib><creatorcontrib>Ros, Emilio</creatorcontrib><creatorcontrib>Millán, Olga</creatorcontrib><creatorcontrib>Cofán, Montse</creatorcontrib><creatorcontrib>Rimola, Antoni</creatorcontrib><creatorcontrib>Brunet, Mercè</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guillén, David</au><au>Cofán, Frederic</au><au>Ros, Emilio</au><au>Millán, Olga</au><au>Cofán, Montse</au><au>Rimola, Antoni</au><au>Brunet, Mercè</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of atorvastatin and its metabolite ortho-hydroxyatorvastatin in human plasma by on-line anion-exchange solid-phase extraction and liquid chromatography tandem mass spectrometry</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2009-07-01</date><risdate>2009</risdate><volume>394</volume><issue>6</issue><spage>1687</spage><epage>1696</epage><pages>1687-1696</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>A rapid, sensitive, and specific method was developed and validated using liquid chromatography-tandem mass spectrometry for the simultaneous quantitation of atorvastatin (ATV) and its major metabolite ortho-hydroxyatorvastatin (o-HATV) in human plasma. The sample preparation involved a liquid-liquid extraction without chlorinated solvents and an on-line solid-phase extraction exploring the possibilities that anion exchange offers. The analytical method presented intraday and day-to-day variation below 10%; intraday and day-to-day accuracy stood between 94% and 105%; the limit of quantification was 0.1 ng/mL for ATV and 0.5 ng/mL for o-HATV; and the recovery was above 75% for both molecules. This method was applied successfully to quantitate ATV and o-HATV concentrations in an unstudied renal transplant recipient cohort treated with an immunosuppressive regime of tacrolimus and mycophenolic acid and a cohort of hypercholesterolemic patients included in the study as a control group. It can be used to evaluate patient adherence, drug-drug interactions, and pharmacokinetic/pharmacodynamic relationships. The results in our study showed that ATV and o-HATV levels in the renal transplant group were significantly increased (p < 0.001), compared to the hypercholesterolemic group.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19506841</pmid><doi>10.1007/s00216-009-2852-3</doi><tpages>10</tpages></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2009-07, Vol.394 (6), p.1687-1696 |
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| subjects | Adult Aged All terrain vehicles Analytical Chemistry Anions - chemistry Atorvastatin Calcium Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatographic methods and physical methods associated with chromatography Chromatography, Liquid - methods Exact sciences and technology Extraction Female Food Science Heptanoic Acids - blood Heptanoic Acids - chemistry Heptanoic Acids - metabolism Human Humans Laboratory Medicine Male Mass spectrometry Mathematical analysis Metabolites Middle Aged Monitoring/Environmental Analysis On-line systems Online Systems Original Paper Other chromatographic methods Pyrroles - blood Pyrroles - chemistry Pyrroles - metabolism Solid Phase Extraction - methods Spectrometric and optical methods Tandem Mass Spectrometry - methods Transplants |
| title | Determination of atorvastatin and its metabolite ortho-hydroxyatorvastatin in human plasma by on-line anion-exchange solid-phase extraction and liquid chromatography tandem mass spectrometry |
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