Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids)
Our aim was to assess the suitability of ultra-high performance liquid chromatography (UHPLC) for the simultaneous determination of biomarkers of vitamins A (retinol, retinyl esters), E (α- and γ-tocopherol), D (25-OH-vitamin D), and the major carotenoids in human serum to be used in clinical practi...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2010-06, Vol.397 (3), p.1389-1393 |
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| creator | Granado-Lorencio, F Herrero-Barbudo, C Blanco-Navarro, I Pérez-Sacristán, B |
| description | Our aim was to assess the suitability of ultra-high performance liquid chromatography (UHPLC) for the simultaneous determination of biomarkers of vitamins A (retinol, retinyl esters), E (α- and γ-tocopherol), D (25-OH-vitamin D), and the major carotenoids in human serum to be used in clinical practice. UHPLC analysis was performed on HSS T3 column (2.1 × 100 mm; 1.8 μm) using gradient elution and UV-VIS detection. The system allows the simultaneous determination of retinol, retinyl palmitate, 25-OH-vitamin D, α- and γ-tocopherol, lutein plus zeaxanthin, α-carotene, β-carotene, α- and β-cryptoxanthin and lycopene. The method showed a good linearity over the physiological range with an adequate accuracy in samples from quality control programs. Suitability of the method in clinical practice was tested by analyzing samples (n = 286) from patients. In conclusion, UHPLC constitutes a reliable approach for nutrient/biomarker profiling allowing the rapid, simultaneous and low-cost determination of vitamins A, E, and D (including vitamers and ester forms) and the major carotenoids in clinical practice. |
| doi_str_mv | 10.1007/s00216-010-3655-2 |
| format | Article |
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UHPLC analysis was performed on HSS T3 column (2.1 × 100 mm; 1.8 μm) using gradient elution and UV-VIS detection. The system allows the simultaneous determination of retinol, retinyl palmitate, 25-OH-vitamin D, α- and γ-tocopherol, lutein plus zeaxanthin, α-carotene, β-carotene, α- and β-cryptoxanthin and lycopene. The method showed a good linearity over the physiological range with an adequate accuracy in samples from quality control programs. Suitability of the method in clinical practice was tested by analyzing samples (n = 286) from patients. In conclusion, UHPLC constitutes a reliable approach for nutrient/biomarker profiling allowing the rapid, simultaneous and low-cost determination of vitamins A, E, and D (including vitamers and ester forms) and the major carotenoids in clinical practice.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-010-3655-2</identifier><identifier>PMID: 20358182</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>25-OH-vitamin D ; Alfacalcidol ; Analysis ; Analytical Chemistry ; Biochemistry ; Biological markers ; Calcifediol ; Carotenes ; Carotenoids ; Carotenoids - blood ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, High Pressure Liquid - methods ; Comparative analysis ; Exact sciences and technology ; Food Science ; High performance liquid chromatography ; Humans ; Laboratory Medicine ; Linear Models ; Liquid chromatography ; Lycopene ; Mathematical analysis ; Methods ; Monitoring/Environmental Analysis ; Nutrition ; Nutritional aspects ; nutritional status ; Other chromatographic methods ; Physiological aspects ; Product/Service Evaluations ; Sensitivity and Specificity ; Serum ; Spectrometric and optical methods ; Technical Note ; Tocopherol ; Tocopherols ; UHPLC ; Vitamin A ; Vitamin A - blood ; Vitamin D ; Vitamin D - blood ; Vitamin E - blood ; Vitamins ; Vitamins - blood</subject><ispartof>Analytical and bioanalytical chemistry, 2010-06, Vol.397 (3), p.1389-1393</ispartof><rights>Springer-Verlag 2010</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2010 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-d31ce99b2b4cb15df2821b8eb127554c1e0bf1b1e962cbdaa2fdb9fa83e438a03</citedby><cites>FETCH-LOGICAL-c529t-d31ce99b2b4cb15df2821b8eb127554c1e0bf1b1e962cbdaa2fdb9fa83e438a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-010-3655-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-010-3655-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22835651$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20358182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Granado-Lorencio, F</creatorcontrib><creatorcontrib>Herrero-Barbudo, C</creatorcontrib><creatorcontrib>Blanco-Navarro, I</creatorcontrib><creatorcontrib>Pérez-Sacristán, B</creatorcontrib><title>Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids)</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Our aim was to assess the suitability of ultra-high performance liquid chromatography (UHPLC) for the simultaneous determination of biomarkers of vitamins A (retinol, retinyl esters), E (α- and γ-tocopherol), D (25-OH-vitamin D), and the major carotenoids in human serum to be used in clinical practice. UHPLC analysis was performed on HSS T3 column (2.1 × 100 mm; 1.8 μm) using gradient elution and UV-VIS detection. The system allows the simultaneous determination of retinol, retinyl palmitate, 25-OH-vitamin D, α- and γ-tocopherol, lutein plus zeaxanthin, α-carotene, β-carotene, α- and β-cryptoxanthin and lycopene. The method showed a good linearity over the physiological range with an adequate accuracy in samples from quality control programs. Suitability of the method in clinical practice was tested by analyzing samples (n = 286) from patients. In conclusion, UHPLC constitutes a reliable approach for nutrient/biomarker profiling allowing the rapid, simultaneous and low-cost determination of vitamins A, E, and D (including vitamers and ester forms) and the major carotenoids in clinical practice.</description><subject>25-OH-vitamin D</subject><subject>Alfacalcidol</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Biological markers</subject><subject>Calcifediol</subject><subject>Carotenes</subject><subject>Carotenoids</subject><subject>Carotenoids - blood</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Comparative analysis</subject><subject>Exact sciences and technology</subject><subject>Food Science</subject><subject>High performance liquid chromatography</subject><subject>Humans</subject><subject>Laboratory Medicine</subject><subject>Linear Models</subject><subject>Liquid chromatography</subject><subject>Lycopene</subject><subject>Mathematical analysis</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Nutrition</subject><subject>Nutritional aspects</subject><subject>nutritional status</subject><subject>Other chromatographic methods</subject><subject>Physiological aspects</subject><subject>Product/Service Evaluations</subject><subject>Sensitivity and Specificity</subject><subject>Serum</subject><subject>Spectrometric and optical methods</subject><subject>Technical Note</subject><subject>Tocopherol</subject><subject>Tocopherols</subject><subject>UHPLC</subject><subject>Vitamin A</subject><subject>Vitamin A - blood</subject><subject>Vitamin D</subject><subject>Vitamin D - blood</subject><subject>Vitamin E - blood</subject><subject>Vitamins</subject><subject>Vitamins - blood</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkl1rFDEUhgdRbK3-AG80UMQKOzUnmZmduVxq_YCCF7XX4eRrN2Vmsk0yhf07_lIz7FoRpJKLhJznPW84eYviNdBzoHT5MVLKoCkp0JI3dV2yJ8UxNNCWrKnp04dzxY6KFzHeUgp1C83z4ohRnk8tOy5-Xk8uoXS9SzviLZn6FLDcuPWGbE2wPgw4KkN6dzc5TdQm-AGTXwfcbnYkl0naGKJNMmFwIybnx7mLxVRG30-yN2ScUnBzAXsSE6YpkrP77Jn5SFYLcrkgnxYER03cqN2901MGFQafzOidjh9eFs8s9tG8Ouwnxc3nyx8XX8ur71--XayuSlWzLpWagzJdJ5mslIRaW9YykK2RwJZ1XSkwVFqQYLqGKakRmdWys9hyU_EWKT8p3u_7boO_m0xMYnBRmb7H0fgpimWdhwy84_8nOa8BGMzk2aMkLKuqWbKune1P9-gaeyPcaH3-CTXjYlVRDk3DWZup839QeWkzOOVHY12-_0sAe4EKPsZgrNgGN2DYCaBiTpHYp0jkFIk5RYJlzZvDqyc5GP2g-B2bDLw7ABgV9jbkkLj4h8u-9Tyrk4LtuZhL49oEceunkJMQH3V_uxdZ9ALXITe-uWYUOIW2Yqyr-C80GelW</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Granado-Lorencio, F</creator><creator>Herrero-Barbudo, C</creator><creator>Blanco-Navarro, I</creator><creator>Pérez-Sacristán, B</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope><scope>C1K</scope></search><sort><creationdate>20100601</creationdate><title>Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids)</title><author>Granado-Lorencio, F ; 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UHPLC analysis was performed on HSS T3 column (2.1 × 100 mm; 1.8 μm) using gradient elution and UV-VIS detection. The system allows the simultaneous determination of retinol, retinyl palmitate, 25-OH-vitamin D, α- and γ-tocopherol, lutein plus zeaxanthin, α-carotene, β-carotene, α- and β-cryptoxanthin and lycopene. The method showed a good linearity over the physiological range with an adequate accuracy in samples from quality control programs. Suitability of the method in clinical practice was tested by analyzing samples (n = 286) from patients. In conclusion, UHPLC constitutes a reliable approach for nutrient/biomarker profiling allowing the rapid, simultaneous and low-cost determination of vitamins A, E, and D (including vitamers and ester forms) and the major carotenoids in clinical practice.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>20358182</pmid><doi>10.1007/s00216-010-3655-2</doi><tpages>5</tpages></addata></record> |
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| ispartof | Analytical and bioanalytical chemistry, 2010-06, Vol.397 (3), p.1389-1393 |
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| subjects | 25-OH-vitamin D Alfacalcidol Analysis Analytical Chemistry Biochemistry Biological markers Calcifediol Carotenes Carotenoids Carotenoids - blood Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Comparative analysis Exact sciences and technology Food Science High performance liquid chromatography Humans Laboratory Medicine Linear Models Liquid chromatography Lycopene Mathematical analysis Methods Monitoring/Environmental Analysis Nutrition Nutritional aspects nutritional status Other chromatographic methods Physiological aspects Product/Service Evaluations Sensitivity and Specificity Serum Spectrometric and optical methods Technical Note Tocopherol Tocopherols UHPLC Vitamin A Vitamin A - blood Vitamin D Vitamin D - blood Vitamin E - blood Vitamins Vitamins - blood |
| title | Suitability of ultra-high performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids) |
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