reusable liposome array and its application to assay of growth-hormone-related peptides

We describe a reusable liposome array based on the formation of cleavable disulfide cross-links between liposomes and the surface of a glass slip. The N-succinimidyl 3-(2-pyridyldithio)-propionate (SPDP)-modified liposomes encapsulating a pH-sensitive fluorescence dye were immobilized on a 3-mercapt...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2010-06, Vol.397 (3), p.1377-1381
Hauptverfasser:	Shoji, Atsushi, Sugimoto, Erika, Orita, Sayaka, Nozawa, Keiichiro, Yanagida, Akio, Shibusawa, Yoichi, Sugawara, Masao
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical Chemistry Arrays Assaying Avidin - chemistry Biochemistry Biosensing Techniques - methods Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Cross-Linking Reagents - chemistry Crosslinking Disulfides - chemistry Exact sciences and technology Fluorescence Fluorimetry Food Science Glass - chemistry Gramicidin - chemistry Gramicidin channel Gramicidins Growth Growth Hormone - analysis Growth hormone releasing factor (GHRF) Growth Hormone-Releasing Hormone - analysis Hydrogen-ion concentration Immunoassay Immunoassay - methods Laboratory Medicine Limit of Detection Liposome array Liposomes Liposomes - chemistry Materials Mathematical analysis Methods Monitoring/Environmental Analysis Peptides Physiological aspects Production processes Properties Proteins Reusable Sensitivity and Specificity Somatostatin Somatostatin - analysis Somatotropin Somatotropin releasing hormone Spectrometric and optical methods Surface active agents Technical Note
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