Continuous fractionation of a two-component mixture by zone electrophoresis
Synchronized continuous-flow zone electrophoresis is a recently demonstrated tool for performing electrophoretic fractionation of a complex sample. The method resembles free flow electrophoresis, but unlike in that technique, no mechanical fluid pumping is required. Instead, fast electrokinetic flow...
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| Veröffentlicht in: | Electrophoresis 2009-12, Vol.30 (24), p.4187-4194 |
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| container_title | Electrophoresis |
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| creator | Zalewski, Dawid R Gardeniers, Han J.G.E |
| description | Synchronized continuous-flow zone electrophoresis is a recently demonstrated tool for performing electrophoretic fractionation of a complex sample. The method resembles free flow electrophoresis, but unlike in that technique, no mechanical fluid pumping is required. Instead, fast electrokinetic flow switching is used to produce complex stream patterns, which results in lateral separation of components in a separation chamber. Here a solution is presented which allows for simultaneous collection of two fractions in synchronized continuous-flow zone electrophoresis. The method is demonstrated on a model mixture, with subsequent evaluation of the collected fractions purity by MCE. The necessary theoretical background is provided including both steering schemes and calculations of optimum operating points. |
| doi_str_mv | 10.1002/elps.200900345 |
| format | Article |
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| ispartof | Electrophoresis, 2009-12, Vol.30 (24), p.4187-4194 |
| issn | 0173-0835 1522-2683 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Electrokinetics Electrophoresis Electrophoresis - methods Fluid flow Fractionation Free flow electrophoresis Lab-On-A-Chip Devices Mathematical models Separation Streams Zone electrophoresis |
| title | Continuous fractionation of a two-component mixture by zone electrophoresis |
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