Ultrastructural appearance of red cells frozen with glycerol for clinical use

Freeze-fracture replicas prepared from full units of red cells frozen with glycerol under clinical conditions suggest that the cells are found in a concentrated glycerol phase and so are physically separated from the extracellular ice which formed during freezing. The cells possess a relatively smoo...

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Veröffentlicht in:	Cryobiology 1980-10, Vol.17 (5), p.448-457
Hauptverfasser:	Allen, E.D., Weatherbee, L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Blood Preservation Erythrocytes - ultrastructure Freeze Fracturing Freezing Glycerol Humans
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description	Freeze-fracture replicas prepared from full units of red cells frozen with glycerol under clinical conditions suggest that the cells are found in a concentrated glycerol phase and so are physically separated from the extracellular ice which formed during freezing. The cells possess a relatively smooth cytoplasmic surface with no evidence of intracellular ice. Half-membrane views of PF faces indicate that the intramembrane particles are aggregated.
doi_str_mv	10.1016/0011-2240(80)90054-1
format	Article
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source	MEDLINE; Elsevier ScienceDirect Journals
subjects	Blood Preservation Erythrocytes - ultrastructure Freeze Fracturing Freezing Glycerol Humans
title	Ultrastructural appearance of red cells frozen with glycerol for clinical use
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