Studies of liposome interactions with rat thymocytes
1. The consequences of incubating liposomes with rat thymocytes have been studied using liposomes of dipalmitoylphosphatidylcholine and cholesterol or dipalmitoylphosphatidylcholine only. 2. Dipalmitoylphosphatidylcholine‐cholesterol liposomes do not bind to the cells and can be removed by washing....
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| Veröffentlicht in: | European journal of biochemistry 1980-09, Vol.110 (2), p.579-585 |
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| container_end_page | 585 |
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| container_issue | 2 |
| container_start_page | 579 |
| container_title | European journal of biochemistry |
| container_volume | 110 |
| creator | KRAMERS, Mary T. C. PATRICK, John BOTTOMLEY, Juliana M. QUINN, Peter J. CHAPMAN, Dennis |
| description | 1. The consequences of incubating liposomes with rat thymocytes have been studied using liposomes of dipalmitoylphosphatidylcholine and cholesterol or dipalmitoylphosphatidylcholine only.
2. Dipalmitoylphosphatidylcholine‐cholesterol liposomes do not bind to the cells and can be removed by washing. An increase in cellular cholesterol is observed. However dipalmitoylphosphatidylcholine liposomes bind rapidly to the cells and cannot be removed by repeated washing. Cholesterol is removed from the cells.
3. There are small changes in intracellular cations in the cholesterol‐enriched cells, but no transport studies have been made. Cells depleted of cholesterol lose K+ with little change in intracellular Na+. Na+ influx is increased. The majority of this increase appears to be ouabain‐sensitive, indicating an increase in pump‐mediated Na+ influx, K+ influx is reduced.
4. The significance of these results is discussed. |
| doi_str_mv | 10.1111/j.1432-1033.1980.tb04901.x |
| format | Article |
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2. Dipalmitoylphosphatidylcholine‐cholesterol liposomes do not bind to the cells and can be removed by washing. An increase in cellular cholesterol is observed. However dipalmitoylphosphatidylcholine liposomes bind rapidly to the cells and cannot be removed by repeated washing. Cholesterol is removed from the cells.
3. There are small changes in intracellular cations in the cholesterol‐enriched cells, but no transport studies have been made. Cells depleted of cholesterol lose K+ with little change in intracellular Na+. Na+ influx is increased. The majority of this increase appears to be ouabain‐sensitive, indicating an increase in pump‐mediated Na+ influx, K+ influx is reduced.
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2. Dipalmitoylphosphatidylcholine‐cholesterol liposomes do not bind to the cells and can be removed by washing. An increase in cellular cholesterol is observed. However dipalmitoylphosphatidylcholine liposomes bind rapidly to the cells and cannot be removed by repeated washing. Cholesterol is removed from the cells.
3. There are small changes in intracellular cations in the cholesterol‐enriched cells, but no transport studies have been made. Cells depleted of cholesterol lose K+ with little change in intracellular Na+. Na+ influx is increased. The majority of this increase appears to be ouabain‐sensitive, indicating an increase in pump‐mediated Na+ influx, K+ influx is reduced.
4. The significance of these results is discussed.</description><subject>Animals</subject><subject>Biological Transport, Active - drug effects</subject><subject>Cholesterol - pharmacology</subject><subject>Female</subject><subject>Freeze Fracturing</subject><subject>Kinetics</subject><subject>Lipids - analysis</subject><subject>Liposomes</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Phosphatidylcholines - pharmacology</subject><subject>Potassium - metabolism</subject><subject>Rats</subject><subject>Sodium - metabolism</subject><subject>Thymus Gland - drug effects</subject><subject>Thymus Gland - metabolism</subject><subject>Thymus Gland - ultrastructure</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMtOwzAQRS0EgvL4BFDEgl2CJxM7NRsEpTykSiyAteU4NrhK6hK7avv3pGrVPbOZxb1zRjqEXAPNoJ_baQYF5ilQxAzEkGaxooWgkK0OyGAfHZIBpVCkuWD8hJyGMKWUcsHLY3JcFiigZANSfMRF7UxIvE0aN_fBtyZxs2g6paPzs5AsXfxJOhWT-LNuvV5HE87JkVVNMBe7fUa-nsefo9d08v7yNnqYpBoFitRYoakF5KKqOGMUYWh1TUXO8xyHUHPF0ajKFpoL0FAwbgxDVaDOlVU14Bm52XLnnf9dmBBl64I2TaNmxi-CLBkyyBn2xbttUXc-hM5YOe9cq7q1BCo3yuRUbrzIjRe5USZ3yuSqP77cfVlUran3pztHfX6_zZeuMet_kOXz-PGDlaInXG0JVnmpvjsX5NO47wLlbEhLxD-6uYR4</recordid><startdate>198009</startdate><enddate>198009</enddate><creator>KRAMERS, Mary T. C.</creator><creator>PATRICK, John</creator><creator>BOTTOMLEY, Juliana M.</creator><creator>QUINN, Peter J.</creator><creator>CHAPMAN, Dennis</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198009</creationdate><title>Studies of liposome interactions with rat thymocytes</title><author>KRAMERS, Mary T. C. ; PATRICK, John ; BOTTOMLEY, Juliana M. ; QUINN, Peter J. ; CHAPMAN, Dennis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3939-ef9c0f1369bb6550318fcd092622381d6a63eabf4c691c1456ee53a43c2afad13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Biological Transport, Active - drug effects</topic><topic>Cholesterol - pharmacology</topic><topic>Female</topic><topic>Freeze Fracturing</topic><topic>Kinetics</topic><topic>Lipids - analysis</topic><topic>Liposomes</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Phosphatidylcholines - pharmacology</topic><topic>Potassium - metabolism</topic><topic>Rats</topic><topic>Sodium - metabolism</topic><topic>Thymus Gland - drug effects</topic><topic>Thymus Gland - metabolism</topic><topic>Thymus Gland - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KRAMERS, Mary T. C.</creatorcontrib><creatorcontrib>PATRICK, John</creatorcontrib><creatorcontrib>BOTTOMLEY, Juliana M.</creatorcontrib><creatorcontrib>QUINN, Peter J.</creatorcontrib><creatorcontrib>CHAPMAN, Dennis</creatorcontrib><creatorcontrib>Royal Free Hospital, London (UK). Medical School. Dept. of Biochemistry</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KRAMERS, Mary T. C.</au><au>PATRICK, John</au><au>BOTTOMLEY, Juliana M.</au><au>QUINN, Peter J.</au><au>CHAPMAN, Dennis</au><aucorp>Royal Free Hospital, London (UK). Medical School. Dept. of Biochemistry</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies of liposome interactions with rat thymocytes</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1980-09</date><risdate>1980</risdate><volume>110</volume><issue>2</issue><spage>579</spage><epage>585</epage><pages>579-585</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><abstract>1. The consequences of incubating liposomes with rat thymocytes have been studied using liposomes of dipalmitoylphosphatidylcholine and cholesterol or dipalmitoylphosphatidylcholine only.
2. Dipalmitoylphosphatidylcholine‐cholesterol liposomes do not bind to the cells and can be removed by washing. An increase in cellular cholesterol is observed. However dipalmitoylphosphatidylcholine liposomes bind rapidly to the cells and cannot be removed by repeated washing. Cholesterol is removed from the cells.
3. There are small changes in intracellular cations in the cholesterol‐enriched cells, but no transport studies have been made. Cells depleted of cholesterol lose K+ with little change in intracellular Na+. Na+ influx is increased. The majority of this increase appears to be ouabain‐sensitive, indicating an increase in pump‐mediated Na+ influx, K+ influx is reduced.
4. The significance of these results is discussed.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7439175</pmid><doi>10.1111/j.1432-1033.1980.tb04901.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2956 |
| ispartof | European journal of biochemistry, 1980-09, Vol.110 (2), p.579-585 |
| issn | 0014-2956 1432-1033 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biological Transport, Active - drug effects Cholesterol - pharmacology Female Freeze Fracturing Kinetics Lipids - analysis Liposomes Male Microscopy, Electron Phosphatidylcholines - pharmacology Potassium - metabolism Rats Sodium - metabolism Thymus Gland - drug effects Thymus Gland - metabolism Thymus Gland - ultrastructure |
| title | Studies of liposome interactions with rat thymocytes |
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