Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product
The avian sarcoma virus-transforming gene product (pp60 src) appears potentially able to mediate cell transformation via phosphorylation since it is tightly associated with a protein kinase activity. We have searched for and have been able to identify a normal cellular protein that appears to be a s...
Gespeichert in:
| Veröffentlicht in: | Cell 1980-01, Vol.21 (3), p.829-836 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 836 |
|---|---|
| container_issue | 3 |
| container_start_page | 829 |
| container_title | Cell |
| container_volume | 21 |
| creator | Erikson, Eleanor Erikson, R.L. |
| description | The avian sarcoma virus-transforming gene product (pp60
src) appears potentially able to mediate cell transformation via phosphorylation since it is tightly associated with a protein kinase activity. We have searched for and have been able to identify a normal cellular protein that appears to be a substrate of pp60
src. The phosphorylation of this protein (34K) is transformation-specific in ASV-transformed cells of both avian and mammalian origin. Moreover, the 34K polypeptide serves as a substrate for the pp60
src phosphotransferase activity in vitro and is phosphorylated at a site identical to the major site of phosphorylation in vivo. These data suggest that upon transformation the 34,000-dalton protein is phosphorylated directly as a result of pp60
src activity. |
| doi_str_mv | 10.1016/0092-8674(80)90446-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75350380</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0092867480904468</els_id><sourcerecordid>75350380</sourcerecordid><originalsourceid>FETCH-LOGICAL-c380t-b9c3f722a8269db93c8e7ae9a96ad35bf11667d8f37133aff03382d176667f143</originalsourceid><addsrcrecordid>eNp9kEtv3CAURlHVKJ08_kFbsarShVswNo9NpCrqI1KkLNqsEYbLhMo2U8Ajzb8v7oyy7AIhuOd-Fw5Cbyn5RAnlnwlRbSO56G4k-ahI1_FGvkIbSpRoOira12jzgrxBFzn_JoTIvu_P0Tlv-45ztUHp3sFcgg_WlBBnHD022MI4LqNJeJdigTDjvAy5JFMA755jrisdxnpyeDjg8gzY7IOplEk2TgbvQ1pyU_k5-5imMG_xFmZY09xiyxU682bMcH3aL9HTt6-_7n40D4_f7---PDSWSVKaQVnmRdsa2XLlBsWsBGFAGcWNY_3gKeVcOOmZoIwZ7wljsnVU8Hrtaccu0Ydjbp37Z4Fc9BTy-jUzQ1yyFj3rSR1Vwe4I2hRzTuD1LoXJpIOmRK-q9epRrx61JPqfai1r27tT_jJM4F6aTm5r_f2x7k3UZptC1k8_qZItEa2Q_Tr39ghAtbAPkHS2AWYLLiSwRbsY_v-Cvz2hmOE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75350380</pqid></control><display><type>article</type><title>Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Erikson, Eleanor ; Erikson, R.L.</creator><creatorcontrib>Erikson, Eleanor ; Erikson, R.L.</creatorcontrib><description>The avian sarcoma virus-transforming gene product (pp60
src) appears potentially able to mediate cell transformation via phosphorylation since it is tightly associated with a protein kinase activity. We have searched for and have been able to identify a normal cellular protein that appears to be a substrate of pp60
src. The phosphorylation of this protein (34K) is transformation-specific in ASV-transformed cells of both avian and mammalian origin. Moreover, the 34K polypeptide serves as a substrate for the pp60
src phosphotransferase activity in vitro and is phosphorylated at a site identical to the major site of phosphorylation in vivo. These data suggest that upon transformation the 34,000-dalton protein is phosphorylated directly as a result of pp60
src activity.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(80)90446-8</identifier><identifier>PMID: 6254669</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Avian Sarcoma Viruses - metabolism ; Cell Line ; Cell Transformation, Neoplastic ; Cell Transformation, Viral ; Chick Embryo ; Phosphoproteins - analysis ; Phosphoproteins - metabolism ; Phosphorylation ; Tyrosine - metabolism ; Viral Proteins - analysis ; Viral Proteins - metabolism</subject><ispartof>Cell, 1980-01, Vol.21 (3), p.829-836</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-b9c3f722a8269db93c8e7ae9a96ad35bf11667d8f37133aff03382d176667f143</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0092867480904468$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6254669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erikson, Eleanor</creatorcontrib><creatorcontrib>Erikson, R.L.</creatorcontrib><title>Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product</title><title>Cell</title><addtitle>Cell</addtitle><description>The avian sarcoma virus-transforming gene product (pp60
src) appears potentially able to mediate cell transformation via phosphorylation since it is tightly associated with a protein kinase activity. We have searched for and have been able to identify a normal cellular protein that appears to be a substrate of pp60
src. The phosphorylation of this protein (34K) is transformation-specific in ASV-transformed cells of both avian and mammalian origin. Moreover, the 34K polypeptide serves as a substrate for the pp60
src phosphotransferase activity in vitro and is phosphorylated at a site identical to the major site of phosphorylation in vivo. These data suggest that upon transformation the 34,000-dalton protein is phosphorylated directly as a result of pp60
src activity.</description><subject>Animals</subject><subject>Avian Sarcoma Viruses - metabolism</subject><subject>Cell Line</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cell Transformation, Viral</subject><subject>Chick Embryo</subject><subject>Phosphoproteins - analysis</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphorylation</subject><subject>Tyrosine - metabolism</subject><subject>Viral Proteins - analysis</subject><subject>Viral Proteins - metabolism</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv3CAURlHVKJ08_kFbsarShVswNo9NpCrqI1KkLNqsEYbLhMo2U8Ajzb8v7oyy7AIhuOd-Fw5Cbyn5RAnlnwlRbSO56G4k-ahI1_FGvkIbSpRoOira12jzgrxBFzn_JoTIvu_P0Tlv-45ztUHp3sFcgg_WlBBnHD022MI4LqNJeJdigTDjvAy5JFMA755jrisdxnpyeDjg8gzY7IOplEk2TgbvQ1pyU_k5-5imMG_xFmZY09xiyxU682bMcH3aL9HTt6-_7n40D4_f7---PDSWSVKaQVnmRdsa2XLlBsWsBGFAGcWNY_3gKeVcOOmZoIwZ7wljsnVU8Hrtaccu0Ydjbp37Z4Fc9BTy-jUzQ1yyFj3rSR1Vwe4I2hRzTuD1LoXJpIOmRK-q9epRrx61JPqfai1r27tT_jJM4F6aTm5r_f2x7k3UZptC1k8_qZItEa2Q_Tr39ghAtbAPkHS2AWYLLiSwRbsY_v-Cvz2hmOE</recordid><startdate>19800101</startdate><enddate>19800101</enddate><creator>Erikson, Eleanor</creator><creator>Erikson, R.L.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800101</creationdate><title>Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product</title><author>Erikson, Eleanor ; Erikson, R.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-b9c3f722a8269db93c8e7ae9a96ad35bf11667d8f37133aff03382d176667f143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Avian Sarcoma Viruses - metabolism</topic><topic>Cell Line</topic><topic>Cell Transformation, Neoplastic</topic><topic>Cell Transformation, Viral</topic><topic>Chick Embryo</topic><topic>Phosphoproteins - analysis</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphorylation</topic><topic>Tyrosine - metabolism</topic><topic>Viral Proteins - analysis</topic><topic>Viral Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erikson, Eleanor</creatorcontrib><creatorcontrib>Erikson, R.L.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erikson, Eleanor</au><au>Erikson, R.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1980-01-01</date><risdate>1980</risdate><volume>21</volume><issue>3</issue><spage>829</spage><epage>836</epage><pages>829-836</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>The avian sarcoma virus-transforming gene product (pp60
src) appears potentially able to mediate cell transformation via phosphorylation since it is tightly associated with a protein kinase activity. We have searched for and have been able to identify a normal cellular protein that appears to be a substrate of pp60
src. The phosphorylation of this protein (34K) is transformation-specific in ASV-transformed cells of both avian and mammalian origin. Moreover, the 34K polypeptide serves as a substrate for the pp60
src phosphotransferase activity in vitro and is phosphorylated at a site identical to the major site of phosphorylation in vivo. These data suggest that upon transformation the 34,000-dalton protein is phosphorylated directly as a result of pp60
src activity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6254669</pmid><doi>10.1016/0092-8674(80)90446-8</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 1980-01, Vol.21 (3), p.829-836 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75350380 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Avian Sarcoma Viruses - metabolism Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Chick Embryo Phosphoproteins - analysis Phosphoproteins - metabolism Phosphorylation Tyrosine - metabolism Viral Proteins - analysis Viral Proteins - metabolism |
| title | Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T12%3A42%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20a%20cellular%20protein%20substrate%20phosphorylated%20by%20the%20avian%20sarcoma%20virus-transforming%20gene%20product&rft.jtitle=Cell&rft.au=Erikson,%20Eleanor&rft.date=1980-01-01&rft.volume=21&rft.issue=3&rft.spage=829&rft.epage=836&rft.pages=829-836&rft.issn=0092-8674&rft.eissn=1097-4172&rft_id=info:doi/10.1016/0092-8674(80)90446-8&rft_dat=%3Cproquest_cross%3E75350380%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75350380&rft_id=info:pmid/6254669&rft_els_id=0092867480904468&rfr_iscdi=true |