Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes
Human B lymphocytes are immortalized by Epstein–Barr virus (EBV, ref. 1). The virus can be used to establish lymphoblastoid cell lines that produce and actively secrete specific antibodies 2 . The original method, which we have used for various antigens 2–4 is based on selection of the specific surf...
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| Veröffentlicht in: | Nature (London) 1980-10, Vol.287 (5781), p.443-445 |
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| creator | Steinitz, Michael Izak, Gabriel Cohen, Sara Ehrenfeld, Michael Flechner, Ianco |
| description | Human B lymphocytes are immortalized by Epstein–Barr virus (EBV, ref. 1). The virus can be used to establish lymphoblastoid cell lines that produce and actively secrete specific antibodies
2
. The original method, which we have used for various antigens
2–4
is based on selection of the specific surface antigen receptor-positive lymphocytes from the peripheral blood lymphocytes of a donor who was previously sensitized to the corresponding antigen. Furthermore, by cloning the polyclonal anti-NNP cell Une we have produced human monoclonal antibodies for the first time
in vitro
5
. About 5–20 µg ml
−1
stably produced
5
specific antibody is obtained in the supernatant of the cell lines. This approach can be used for the
in vitro
production of monoclonal human autoimmune antibodies by EBV-immortalized lymphocytes from patients with autoimmune diseases. We demonstrate the continuous production
in vitro
of a monoclonal IgM anti-IgG antibody (rheumatoid factor, r.f.) by a lymphoblastoid cell line established from a patient with rheumatoid arthritis. |
| doi_str_mv | 10.1038/287443a0 |
| format | Article |
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2
. The original method, which we have used for various antigens
2–4
is based on selection of the specific surface antigen receptor-positive lymphocytes from the peripheral blood lymphocytes of a donor who was previously sensitized to the corresponding antigen. Furthermore, by cloning the polyclonal anti-NNP cell Une we have produced human monoclonal antibodies for the first time
in vitro
5
. About 5–20 µg ml
−1
stably produced
5
specific antibody is obtained in the supernatant of the cell lines. This approach can be used for the
in vitro
production of monoclonal human autoimmune antibodies by EBV-immortalized lymphocytes from patients with autoimmune diseases. We demonstrate the continuous production
in vitro
of a monoclonal IgM anti-IgG antibody (rheumatoid factor, r.f.) by a lymphoblastoid cell line established from a patient with rheumatoid arthritis.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/287443a0</identifier><identifier>PMID: 6253801</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Arthritis, Rheumatoid - immunology ; B-Lymphocytes - immunology ; Cell Transformation, Viral ; Clone Cells - immunology ; Herpesvirus 4, Human ; Humanities and Social Sciences ; Humans ; Immunoglobulin M - biosynthesis ; Isoelectric Point ; letter ; multidisciplinary ; Rheumatoid Factor - biosynthesis ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1980-10, Vol.287 (5781), p.443-445</ispartof><rights>Springer Nature Limited 1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-cb93067178616c6a9e6891131dae727723a15229219b8e581e453bea92fa67aa3</citedby><cites>FETCH-LOGICAL-c378t-cb93067178616c6a9e6891131dae727723a15229219b8e581e453bea92fa67aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/287443a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/287443a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6253801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Steinitz, Michael</creatorcontrib><creatorcontrib>Izak, Gabriel</creatorcontrib><creatorcontrib>Cohen, Sara</creatorcontrib><creatorcontrib>Ehrenfeld, Michael</creatorcontrib><creatorcontrib>Flechner, Ianco</creatorcontrib><title>Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Human B lymphocytes are immortalized by Epstein–Barr virus (EBV, ref. 1). The virus can be used to establish lymphoblastoid cell lines that produce and actively secrete specific antibodies
2
. The original method, which we have used for various antigens
2–4
is based on selection of the specific surface antigen receptor-positive lymphocytes from the peripheral blood lymphocytes of a donor who was previously sensitized to the corresponding antigen. Furthermore, by cloning the polyclonal anti-NNP cell Une we have produced human monoclonal antibodies for the first time
in vitro
5
. About 5–20 µg ml
−1
stably produced
5
specific antibody is obtained in the supernatant of the cell lines. This approach can be used for the
in vitro
production of monoclonal human autoimmune antibodies by EBV-immortalized lymphocytes from patients with autoimmune diseases. We demonstrate the continuous production
in vitro
of a monoclonal IgM anti-IgG antibody (rheumatoid factor, r.f.) by a lymphoblastoid cell line established from a patient with rheumatoid arthritis.</description><subject>Arthritis, Rheumatoid - immunology</subject><subject>B-Lymphocytes - immunology</subject><subject>Cell Transformation, Viral</subject><subject>Clone Cells - immunology</subject><subject>Herpesvirus 4, Human</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Immunoglobulin M - biosynthesis</subject><subject>Isoelectric Point</subject><subject>letter</subject><subject>multidisciplinary</subject><subject>Rheumatoid Factor - biosynthesis</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkDtPwzAUhS0EKqUg8QeQPCEYAn4ktjNCVR5SJZbCGt24Dk2V2MV2hvx7jFpYmO5wPh3d8yF0SckdJVzdMyXznAM5QlOaS5HlQsljNCWEqYwoLk7RWQhbQkhBZT5BE8EKrgidotXc2djawQ0B77xbDzq2zmLX4N5ZpztnocN-Y4YeomvXuAEdncf1iBePH1n0YEPjfG_WuBv73cbpMZpwjk4a6IK5ONwZen9arOYv2fLt-XX-sMw0lypmui45EZJKJajQAkojVEkpp2swkknJONCCsZLRslamUNTkBa8NlKwBIQH4DF3ve9PnX4MJserboE3XgTVpUCULzoVgJIE3e1B7F4I3TbXzbQ9-rCipfgRWvwITenXoHOo06w88GEv57T4PKbGfxldbN_hkKfzv-gZE6Hga</recordid><startdate>19801002</startdate><enddate>19801002</enddate><creator>Steinitz, Michael</creator><creator>Izak, Gabriel</creator><creator>Cohen, Sara</creator><creator>Ehrenfeld, Michael</creator><creator>Flechner, Ianco</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19801002</creationdate><title>Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes</title><author>Steinitz, Michael ; Izak, Gabriel ; Cohen, Sara ; Ehrenfeld, Michael ; Flechner, Ianco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-cb93067178616c6a9e6891131dae727723a15229219b8e581e453bea92fa67aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Arthritis, Rheumatoid - immunology</topic><topic>B-Lymphocytes - immunology</topic><topic>Cell Transformation, Viral</topic><topic>Clone Cells - immunology</topic><topic>Herpesvirus 4, Human</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Immunoglobulin M - biosynthesis</topic><topic>Isoelectric Point</topic><topic>letter</topic><topic>multidisciplinary</topic><topic>Rheumatoid Factor - biosynthesis</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Steinitz, Michael</creatorcontrib><creatorcontrib>Izak, Gabriel</creatorcontrib><creatorcontrib>Cohen, Sara</creatorcontrib><creatorcontrib>Ehrenfeld, Michael</creatorcontrib><creatorcontrib>Flechner, Ianco</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Steinitz, Michael</au><au>Izak, Gabriel</au><au>Cohen, Sara</au><au>Ehrenfeld, Michael</au><au>Flechner, Ianco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1980-10-02</date><risdate>1980</risdate><volume>287</volume><issue>5781</issue><spage>443</spage><epage>445</epage><pages>443-445</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>Human B lymphocytes are immortalized by Epstein–Barr virus (EBV, ref. 1). The virus can be used to establish lymphoblastoid cell lines that produce and actively secrete specific antibodies
2
. The original method, which we have used for various antigens
2–4
is based on selection of the specific surface antigen receptor-positive lymphocytes from the peripheral blood lymphocytes of a donor who was previously sensitized to the corresponding antigen. Furthermore, by cloning the polyclonal anti-NNP cell Une we have produced human monoclonal antibodies for the first time
in vitro
5
. About 5–20 µg ml
−1
stably produced
5
specific antibody is obtained in the supernatant of the cell lines. This approach can be used for the
in vitro
production of monoclonal human autoimmune antibodies by EBV-immortalized lymphocytes from patients with autoimmune diseases. We demonstrate the continuous production
in vitro
of a monoclonal IgM anti-IgG antibody (rheumatoid factor, r.f.) by a lymphoblastoid cell line established from a patient with rheumatoid arthritis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>6253801</pmid><doi>10.1038/287443a0</doi><tpages>3</tpages></addata></record> |
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| ispartof | Nature (London), 1980-10, Vol.287 (5781), p.443-445 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature |
| subjects | Arthritis, Rheumatoid - immunology B-Lymphocytes - immunology Cell Transformation, Viral Clone Cells - immunology Herpesvirus 4, Human Humanities and Social Sciences Humans Immunoglobulin M - biosynthesis Isoelectric Point letter multidisciplinary Rheumatoid Factor - biosynthesis Science Science (multidisciplinary) |
| title | Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes |
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