UTILIZATION OF CIBACRON BLUE 3G-A SEPHAROSE 6B IN THE ISOLATION AND ENRICHMENT OF PYRUVATE, PHOSPHATE DIKINASE, ALANINE DEHYDROGENASE, AND PHOSPHOENOLPYRUVATE CARBOXYLASE FROM MITOMYCIN-PRODUCING STREPTOMYCES VERTICILLATUS

Pyruvate, phosphate dikinase (E.C. 2.7.9.1) has been demonstrated in mitomycin-producing Streptomyces verticillatus. Low levels of activity are detectable in noninduced cultures; however, in alanine or pyruvate containing media the enzyme activity is induced 20 to 40 fold. Assays for the dikinase ar...

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Veröffentlicht in:	Journal of antibiotics 1980, Vol.33(8), pp.863-877
Hauptverfasser:	REDMAN, KENT L., HORNEMANN, ULFERT
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine Dehydrogenase Amino Acid Oxidoreductases - isolation & purification Anthracenes Carboxy-Lyases - isolation & purification Chromatography, Affinity Mitomycins - metabolism Phosphoenolpyruvate Carboxylase - isolation & purification Phosphotransferases - isolation & purification Pyruvate, Orthophosphate Dikinase - isolation & purification Streptomyces - enzymology Triazines
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