A Technique for the Flow Cytometric Analysis of Lymphocytes Bearing Histamine Receptors
Histamine receptors have been demonstrated on lymphocyte membranes by a variety of techniques. We now report a method that allows for the flow cytometric analysis of histamine receptors on human peripheral T cells. Histamine is conjugated to fluoresceinated human albumin by the coupling agent ECDI....
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| Veröffentlicht in: | Blood 1980-11, Vol.56 (5), p.923-925 |
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| creator | Osband, Michael E. Cohen, Ellen B. McCaffrey, Ronald P. Shapiro, Howard M. |
| description | Histamine receptors have been demonstrated on lymphocyte membranes by a variety of techniques. We now report a method that allows for the flow cytometric analysis of histamine receptors on human peripheral T cells. Histamine is conjugated to fluoresceinated human albumin by the coupling agent ECDI. This conjugated histamine compound (FHA-his) binds to approximately 45% of T cells. Fluoresceinated human albumin alone (FHA), not conjugated to histamine, does not bind to T cells. In addition, unconjugated histamine can inhibit completely the binding seen with FHA-his. We conclude that this technique demonstrates specific FHA-his binding to histamine receptors on T cells and can be used to determine the number of cells bearing such receptors. In addition, the reagent could be used with a cell sorter to isolate distinct histamine-receptor-bearing (HR+) cells for further immunologic study. |
| doi_str_mv | 10.1182/blood.V56.5.923.923 |
| format | Article |
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We now report a method that allows for the flow cytometric analysis of histamine receptors on human peripheral T cells. Histamine is conjugated to fluoresceinated human albumin by the coupling agent ECDI. This conjugated histamine compound (FHA-his) binds to approximately 45% of T cells. Fluoresceinated human albumin alone (FHA), not conjugated to histamine, does not bind to T cells. In addition, unconjugated histamine can inhibit completely the binding seen with FHA-his. We conclude that this technique demonstrates specific FHA-his binding to histamine receptors on T cells and can be used to determine the number of cells bearing such receptors. 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In addition, the reagent could be used with a cell sorter to isolate distinct histamine-receptor-bearing (HR+) cells for further immunologic study.</description><subject>Blood Cell Count</subject><subject>Fluoresceins - metabolism</subject><subject>Histamine - metabolism</subject><subject>Humans</subject><subject>Receptors, Histamine</subject><subject>Serum Albumin - metabolism</subject><subject>T-Lymphocytes</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9P4zAUxC0EgvLnEyAkn7ilOE7s2Ic9lGoLSJWQUIGj5dgvW6-SuGu7rPLtSWnFkcPoHd7MSPND6Don0zwX9K5uvbfTN8anbCppsdMRmuSMiowQSo7RhBDCs1JW-Rk6j_EvIXlZUHaKTrnkghM6Qe8zvAKz7t2_LeDGB5zWgBet_4_nQ_IdpOAMnvW6HaKL2Dd4OXSbtTdDgojvQQfX_8GPLibduR7wCxjYJB_iJTppdBvh6nAv0Ovi92r-mC2fH57ms2VmCilTBoSLwjLgTSUbDqSmRWnKEhpSiqpkllSgizKvLbWGVY1gFLSQouaVFlZWtLhAt_veTfDjhJhU56KBttU9-G1UFaOSCc5GY7E3muBjDNCoTXCdDoPKidrhVF841YhTMTWi3GlM3Rzqt3UH9jtz4Df-f-3_MG78cBBUNA56A9YFMElZ737s_wRqYYeH</recordid><startdate>198011</startdate><enddate>198011</enddate><creator>Osband, Michael E.</creator><creator>Cohen, Ellen B.</creator><creator>McCaffrey, Ronald P.</creator><creator>Shapiro, Howard M.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198011</creationdate><title>A Technique for the Flow Cytometric Analysis of Lymphocytes Bearing Histamine Receptors</title><author>Osband, Michael E. ; Cohen, Ellen B. ; McCaffrey, Ronald P. ; Shapiro, Howard M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-e0683d5e6f79f6e0b234c44ef048745d07ea341bd2dc57f852ea898b67a8d9723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Blood Cell Count</topic><topic>Fluoresceins - metabolism</topic><topic>Histamine - metabolism</topic><topic>Humans</topic><topic>Receptors, Histamine</topic><topic>Serum Albumin - metabolism</topic><topic>T-Lymphocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osband, Michael E.</creatorcontrib><creatorcontrib>Cohen, Ellen B.</creatorcontrib><creatorcontrib>McCaffrey, Ronald P.</creatorcontrib><creatorcontrib>Shapiro, Howard M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osband, Michael E.</au><au>Cohen, Ellen B.</au><au>McCaffrey, Ronald P.</au><au>Shapiro, Howard M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Technique for the Flow Cytometric Analysis of Lymphocytes Bearing Histamine Receptors</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1980-11</date><risdate>1980</risdate><volume>56</volume><issue>5</issue><spage>923</spage><epage>925</epage><pages>923-925</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>Histamine receptors have been demonstrated on lymphocyte membranes by a variety of techniques. 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| fulltext | fulltext |
| identifier | ISSN: 0006-4971 |
| ispartof | Blood, 1980-11, Vol.56 (5), p.923-925 |
| issn | 0006-4971 1528-0020 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Blood Cell Count Fluoresceins - metabolism Histamine - metabolism Humans Receptors, Histamine Serum Albumin - metabolism T-Lymphocytes |
| title | A Technique for the Flow Cytometric Analysis of Lymphocytes Bearing Histamine Receptors |
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