A radioimmunoassay for human metallothionein
Endogenous metallothionein (Mt) in human body fluids has never been reported due to the lack of a sensitive technique. Described will be a sensitive and accurate radioimmunoassay for human Mt utilizing an antibody developed to purified rat cadmium-binding protein. A high titer antiserum having compl...
Gespeichert in:
| Veröffentlicht in: | Toxicology and applied pharmacology 1980-08, Vol.55 (1), p.94-102 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 102 |
|---|---|
| container_issue | 1 |
| container_start_page | 94 |
| container_title | Toxicology and applied pharmacology |
| container_volume | 55 |
| creator | Chang, Chin C. Vander Mallie, Ronald J. Garvey, Justine S. |
| description | Endogenous metallothionein (Mt) in human body fluids has never been reported due to the lack of a sensitive technique. Described will be a sensitive and accurate radioimmunoassay for human Mt utilizing an antibody developed to purified rat cadmium-binding protein. A high titer antiserum having complete cross-reactivity with human Mt was used at a final dilution of 1:20,000 to detect a routine lower sensitivity of 100–200 pg Mt. The same antiserum was subsequently used to measure Mt in plasma and urine of cadmium-exposed workers. Urinary Mt levels were found to have a close correlation with urinary Cd content,
r = 0.89. |
| doi_str_mv | 10.1016/0041-008X(80)90224-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75290707</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0041008X80902240</els_id><sourcerecordid>75290707</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-4237831565b62b92fd0946192a89afa224d23f9e1f9af8babafbd268ff5cd5333</originalsourceid><addsrcrecordid>eNp9kFtLAzEQhYMotVb_gcI-iYKrk2RveRFK8QYFXxR8C9lcaGSz0WRX6L83taWPPg3DnDlz5kPoHMMtBlzdARQ4B2g-rhq4ZkBIkcMBmmJgVQ6U0kM03UuO0UmMnwDAigJP0KQuCC0xnaKbeRaEst46N_ZexCjWmfEhW41O9JnTg-g6P6ys77XtT9GREV3UZ7s6Q--PD2-L53z5-vSymC9zSct6yJN33VBcVmVbkZYRo9LZCjMiGiaMSEEVoYZpbFLbtKIVplWkaowppSpT8hm63Pp-Bf896jhwZ6PUXSd67cfI65IwqKFOwmIrlMHHGLThX8E6EdYcA99A4hsCfEOAN8D_IHFIaxc7_7F1Wu2XdlTS_H471-nJH6sDj9LqXmplg5YDV97-f-AXJdl1jg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75290707</pqid></control><display><type>article</type><title>A radioimmunoassay for human metallothionein</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Chang, Chin C. ; Vander Mallie, Ronald J. ; Garvey, Justine S.</creator><creatorcontrib>Chang, Chin C. ; Vander Mallie, Ronald J. ; Garvey, Justine S.</creatorcontrib><description>Endogenous metallothionein (Mt) in human body fluids has never been reported due to the lack of a sensitive technique. Described will be a sensitive and accurate radioimmunoassay for human Mt utilizing an antibody developed to purified rat cadmium-binding protein. A high titer antiserum having complete cross-reactivity with human Mt was used at a final dilution of 1:20,000 to detect a routine lower sensitivity of 100–200 pg Mt. The same antiserum was subsequently used to measure Mt in plasma and urine of cadmium-exposed workers. Urinary Mt levels were found to have a close correlation with urinary Cd content,
r = 0.89.</description><identifier>ISSN: 0041-008X</identifier><identifier>EISSN: 1096-0333</identifier><identifier>DOI: 10.1016/0041-008X(80)90224-0</identifier><identifier>PMID: 7423513</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cadmium - blood ; Cadmium - urine ; Humans ; Immune Sera ; Metalloproteins - analysis ; Metallothionein - analysis ; Metallothionein - urine ; Radioimmunoassay</subject><ispartof>Toxicology and applied pharmacology, 1980-08, Vol.55 (1), p.94-102</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-4237831565b62b92fd0946192a89afa224d23f9e1f9af8babafbd268ff5cd5333</citedby><cites>FETCH-LOGICAL-c357t-4237831565b62b92fd0946192a89afa224d23f9e1f9af8babafbd268ff5cd5333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0041-008X(80)90224-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7423513$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Chin C.</creatorcontrib><creatorcontrib>Vander Mallie, Ronald J.</creatorcontrib><creatorcontrib>Garvey, Justine S.</creatorcontrib><title>A radioimmunoassay for human metallothionein</title><title>Toxicology and applied pharmacology</title><addtitle>Toxicol Appl Pharmacol</addtitle><description>Endogenous metallothionein (Mt) in human body fluids has never been reported due to the lack of a sensitive technique. Described will be a sensitive and accurate radioimmunoassay for human Mt utilizing an antibody developed to purified rat cadmium-binding protein. A high titer antiserum having complete cross-reactivity with human Mt was used at a final dilution of 1:20,000 to detect a routine lower sensitivity of 100–200 pg Mt. The same antiserum was subsequently used to measure Mt in plasma and urine of cadmium-exposed workers. Urinary Mt levels were found to have a close correlation with urinary Cd content,
r = 0.89.</description><subject>Cadmium - blood</subject><subject>Cadmium - urine</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Metalloproteins - analysis</subject><subject>Metallothionein - analysis</subject><subject>Metallothionein - urine</subject><subject>Radioimmunoassay</subject><issn>0041-008X</issn><issn>1096-0333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFtLAzEQhYMotVb_gcI-iYKrk2RveRFK8QYFXxR8C9lcaGSz0WRX6L83taWPPg3DnDlz5kPoHMMtBlzdARQ4B2g-rhq4ZkBIkcMBmmJgVQ6U0kM03UuO0UmMnwDAigJP0KQuCC0xnaKbeRaEst46N_ZexCjWmfEhW41O9JnTg-g6P6ys77XtT9GREV3UZ7s6Q--PD2-L53z5-vSymC9zSct6yJN33VBcVmVbkZYRo9LZCjMiGiaMSEEVoYZpbFLbtKIVplWkaowppSpT8hm63Pp-Bf896jhwZ6PUXSd67cfI65IwqKFOwmIrlMHHGLThX8E6EdYcA99A4hsCfEOAN8D_IHFIaxc7_7F1Wu2XdlTS_H471-nJH6sDj9LqXmplg5YDV97-f-AXJdl1jg</recordid><startdate>198008</startdate><enddate>198008</enddate><creator>Chang, Chin C.</creator><creator>Vander Mallie, Ronald J.</creator><creator>Garvey, Justine S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198008</creationdate><title>A radioimmunoassay for human metallothionein</title><author>Chang, Chin C. ; Vander Mallie, Ronald J. ; Garvey, Justine S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-4237831565b62b92fd0946192a89afa224d23f9e1f9af8babafbd268ff5cd5333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Cadmium - blood</topic><topic>Cadmium - urine</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Metalloproteins - analysis</topic><topic>Metallothionein - analysis</topic><topic>Metallothionein - urine</topic><topic>Radioimmunoassay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Chin C.</creatorcontrib><creatorcontrib>Vander Mallie, Ronald J.</creatorcontrib><creatorcontrib>Garvey, Justine S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicology and applied pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Chin C.</au><au>Vander Mallie, Ronald J.</au><au>Garvey, Justine S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A radioimmunoassay for human metallothionein</atitle><jtitle>Toxicology and applied pharmacology</jtitle><addtitle>Toxicol Appl Pharmacol</addtitle><date>1980-08</date><risdate>1980</risdate><volume>55</volume><issue>1</issue><spage>94</spage><epage>102</epage><pages>94-102</pages><issn>0041-008X</issn><eissn>1096-0333</eissn><abstract>Endogenous metallothionein (Mt) in human body fluids has never been reported due to the lack of a sensitive technique. Described will be a sensitive and accurate radioimmunoassay for human Mt utilizing an antibody developed to purified rat cadmium-binding protein. A high titer antiserum having complete cross-reactivity with human Mt was used at a final dilution of 1:20,000 to detect a routine lower sensitivity of 100–200 pg Mt. The same antiserum was subsequently used to measure Mt in plasma and urine of cadmium-exposed workers. Urinary Mt levels were found to have a close correlation with urinary Cd content,
r = 0.89.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7423513</pmid><doi>10.1016/0041-008X(80)90224-0</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-008X |
| ispartof | Toxicology and applied pharmacology, 1980-08, Vol.55 (1), p.94-102 |
| issn | 0041-008X 1096-0333 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75290707 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Cadmium - blood Cadmium - urine Humans Immune Sera Metalloproteins - analysis Metallothionein - analysis Metallothionein - urine Radioimmunoassay |
| title | A radioimmunoassay for human metallothionein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T00%3A46%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20radioimmunoassay%20for%20human%20metallothionein&rft.jtitle=Toxicology%20and%20applied%20pharmacology&rft.au=Chang,%20Chin%20C.&rft.date=1980-08&rft.volume=55&rft.issue=1&rft.spage=94&rft.epage=102&rft.pages=94-102&rft.issn=0041-008X&rft.eissn=1096-0333&rft_id=info:doi/10.1016/0041-008X(80)90224-0&rft_dat=%3Cproquest_cross%3E75290707%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75290707&rft_id=info:pmid/7423513&rft_els_id=0041008X80902240&rfr_iscdi=true |