Stimulating Effect of Nucleoprotein Fraction of Chick Embryo Extract on Homologous Heart Fibroblasts.
Summary 1. A quantity isolation method is described for a chick embryo nucleoprotein fraction based on von Euler and Heller's(10) procedure for liver nucleoproteins. 2. When this fraction was tested in an assay medium of un-dialyzd chicken plasma and dilute un-dialyzed horse serum large outgrow...
Gespeichert in:
| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1953-06, Vol.83 (2), p.390-395 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 395 |
|---|---|
| container_issue | 2 |
| container_start_page | 390 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 83 |
| creator | Kutsky, R. J. |
| description | Summary
1. A quantity isolation method is described for a chick embryo nucleoprotein fraction based on von Euler and Heller's(10) procedure for liver nucleoproteins. 2. When this fraction was tested in an assay medium of un-dialyzd chicken plasma and dilute un-dialyzed horse serum large outgrowths of chick heart fibroblasts were obtained with well-defined alterations in cell morphology and mode of outgrowth. 3. The nucleoprotein fraction described contains protein, 10.3% PNA, 0.3% DNA and has a characteristic nucleoprotein absorption spectrum. 4. High polymer pentose nucleic acid isolated according to Nishioka and Ibuka(9) produced no changes in size or appearance of assay cultures. |
| doi_str_mv | 10.3181/00379727-83-20368 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75280398</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.3181_00379727-83-20368</sage_id><sourcerecordid>75280398</sourcerecordid><originalsourceid>FETCH-LOGICAL-c338t-855a86fbdd35fabde5062f46c59f1d5927ca64e09cf36a0039788ff2e090832f3</originalsourceid><addsrcrecordid>eNp9kM1OwzAQhC0EoqXwAFyQT9xS_FM7zhFVLUWq4ACcLcexS0oSF9uR6Nvj0CJunFba-Wa0OwBcYzSlWOA7hGhe5CTPBM0IolycgDFmlGWUF8UpGA96NgAjcBHCFiHMcsLPwQhTxGckL8bAvMS67RsV624DF9YaHaGz8KnXjXE776KpO7j0SsfadYMyf6_1B1y0pd87uPiKgwSTtHKta9zG9QGujPIRLuvSu7JRIYbpJTizqgnm6jgn4G25eJ2vsvXzw-P8fp1pSkXMBGNKcFtWFWVWlZVhiBM745oVFlesILlWfGZQoS3lKn1X5EJYS9IGCUosnYDbQ266_LM3Icq2Dto0jepMukzmjIjkEgnEB1B7F4I3Vu583Sq_lxjJoVv5260UVP50mzw3x_C-bE315ziWmYDpAQhqY-TW9b5Lz_6T-A3OqYKR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75280398</pqid></control><display><type>article</type><title>Stimulating Effect of Nucleoprotein Fraction of Chick Embryo Extract on Homologous Heart Fibroblasts.</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Kutsky, R. J.</creator><creatorcontrib>Kutsky, R. J.</creatorcontrib><description>Summary
1. A quantity isolation method is described for a chick embryo nucleoprotein fraction based on von Euler and Heller's(10) procedure for liver nucleoproteins. 2. When this fraction was tested in an assay medium of un-dialyzd chicken plasma and dilute un-dialyzed horse serum large outgrowths of chick heart fibroblasts were obtained with well-defined alterations in cell morphology and mode of outgrowth. 3. The nucleoprotein fraction described contains protein, 10.3% PNA, 0.3% DNA and has a characteristic nucleoprotein absorption spectrum. 4. High polymer pentose nucleic acid isolated according to Nishioka and Ibuka(9) produced no changes in size or appearance of assay cultures.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-83-20368</identifier><identifier>PMID: 13064279</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Embryo, Mammalian ; Embryo, Nonmammalian ; Fibroblasts ; Heart ; Nucleoproteins ; Old Medline ; Tissue Culture Techniques</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1953-06, Vol.83 (2), p.390-395</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-855a86fbdd35fabde5062f46c59f1d5927ca64e09cf36a0039788ff2e090832f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13064279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kutsky, R. J.</creatorcontrib><title>Stimulating Effect of Nucleoprotein Fraction of Chick Embryo Extract on Homologous Heart Fibroblasts.</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
1. A quantity isolation method is described for a chick embryo nucleoprotein fraction based on von Euler and Heller's(10) procedure for liver nucleoproteins. 2. When this fraction was tested in an assay medium of un-dialyzd chicken plasma and dilute un-dialyzed horse serum large outgrowths of chick heart fibroblasts were obtained with well-defined alterations in cell morphology and mode of outgrowth. 3. The nucleoprotein fraction described contains protein, 10.3% PNA, 0.3% DNA and has a characteristic nucleoprotein absorption spectrum. 4. High polymer pentose nucleic acid isolated according to Nishioka and Ibuka(9) produced no changes in size or appearance of assay cultures.</description><subject>Embryo, Mammalian</subject><subject>Embryo, Nonmammalian</subject><subject>Fibroblasts</subject><subject>Heart</subject><subject>Nucleoproteins</subject><subject>Old Medline</subject><subject>Tissue Culture Techniques</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1953</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1OwzAQhC0EoqXwAFyQT9xS_FM7zhFVLUWq4ACcLcexS0oSF9uR6Nvj0CJunFba-Wa0OwBcYzSlWOA7hGhe5CTPBM0IolycgDFmlGWUF8UpGA96NgAjcBHCFiHMcsLPwQhTxGckL8bAvMS67RsV624DF9YaHaGz8KnXjXE776KpO7j0SsfadYMyf6_1B1y0pd87uPiKgwSTtHKta9zG9QGujPIRLuvSu7JRIYbpJTizqgnm6jgn4G25eJ2vsvXzw-P8fp1pSkXMBGNKcFtWFWVWlZVhiBM745oVFlesILlWfGZQoS3lKn1X5EJYS9IGCUosnYDbQ266_LM3Icq2Dto0jepMukzmjIjkEgnEB1B7F4I3Vu583Sq_lxjJoVv5260UVP50mzw3x_C-bE315ziWmYDpAQhqY-TW9b5Lz_6T-A3OqYKR</recordid><startdate>195306</startdate><enddate>195306</enddate><creator>Kutsky, R. J.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>195306</creationdate><title>Stimulating Effect of Nucleoprotein Fraction of Chick Embryo Extract on Homologous Heart Fibroblasts.</title><author>Kutsky, R. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-855a86fbdd35fabde5062f46c59f1d5927ca64e09cf36a0039788ff2e090832f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1953</creationdate><topic>Embryo, Mammalian</topic><topic>Embryo, Nonmammalian</topic><topic>Fibroblasts</topic><topic>Heart</topic><topic>Nucleoproteins</topic><topic>Old Medline</topic><topic>Tissue Culture Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kutsky, R. J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kutsky, R. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulating Effect of Nucleoprotein Fraction of Chick Embryo Extract on Homologous Heart Fibroblasts.</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1953-06</date><risdate>1953</risdate><volume>83</volume><issue>2</issue><spage>390</spage><epage>395</epage><pages>390-395</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
1. A quantity isolation method is described for a chick embryo nucleoprotein fraction based on von Euler and Heller's(10) procedure for liver nucleoproteins. 2. When this fraction was tested in an assay medium of un-dialyzd chicken plasma and dilute un-dialyzed horse serum large outgrowths of chick heart fibroblasts were obtained with well-defined alterations in cell morphology and mode of outgrowth. 3. The nucleoprotein fraction described contains protein, 10.3% PNA, 0.3% DNA and has a characteristic nucleoprotein absorption spectrum. 4. High polymer pentose nucleic acid isolated according to Nishioka and Ibuka(9) produced no changes in size or appearance of assay cultures.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>13064279</pmid><doi>10.3181/00379727-83-20368</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1953-06, Vol.83 (2), p.390-395 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75280398 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Embryo, Mammalian Embryo, Nonmammalian Fibroblasts Heart Nucleoproteins Old Medline Tissue Culture Techniques |
| title | Stimulating Effect of Nucleoprotein Fraction of Chick Embryo Extract on Homologous Heart Fibroblasts. |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T09%3A02%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stimulating%20Effect%20of%20Nucleoprotein%20Fraction%20of%20Chick%20Embryo%20Extract%20on%20Homologous%20Heart%20Fibroblasts.&rft.jtitle=Experimental%20biology%20and%20medicine%20(Maywood,%20N.J.)&rft.au=Kutsky,%20R.%20J.&rft.date=1953-06&rft.volume=83&rft.issue=2&rft.spage=390&rft.epage=395&rft.pages=390-395&rft.issn=0037-9727&rft.eissn=1535-3699&rft_id=info:doi/10.3181/00379727-83-20368&rft_dat=%3Cproquest_cross%3E75280398%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75280398&rft_id=info:pmid/13064279&rft_sage_id=10.3181_00379727-83-20368&rfr_iscdi=true |