EFFECT OF SODIUM CHLORIDE ON GENTAMICIN ACCUMULATION BY ESCHERICHIA COLI: CORRELATION WITH BACTERIAL GROWTH AND VIABILITY

EFFECT OF SODIUM CHLORIDE ON GENTAMICIN ACCUMULATION BY ESCHERICHIA COLI: CORRELATION WITH BACTERIAL GROWTH AND VIABILITY

The kinetics of gentamicin accumulation by a sensitive strain of Escherichia coli were investigated at gentamicin concentrations from 0.02 to 200μg/ml. Accumulation with time shows two energy-dependent phases and is saturable. Sodium chloride delays the onset of the second more rapid energy-dependen...

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Veröffentlicht in:Journal of antibiotics 1980, Vol.33(6), pp.604-613
Hauptverfasser: HELLER, ALLEN H., SPECTOR, REYNOLD, AALYSON, MOLLY
Format: Artikel
Sprache:eng
Schlagworte:
Escherichia coli - drug effects
Escherichia coli - growth & development
Escherichia coli - metabolism
Gentamicins - metabolism
Hydrogen-Ion Concentration
Kinetics
Microbial Sensitivity Tests
Sodium Chloride - pharmacology
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container_end_page 613
container_issue 6
container_start_page 604
container_title Journal of antibiotics
container_volume 33
creator HELLER, ALLEN H.
SPECTOR, REYNOLD
AALYSON, MOLLY
description The kinetics of gentamicin accumulation by a sensitive strain of Escherichia coli were investigated at gentamicin concentrations from 0.02 to 200μg/ml. Accumulation with time shows two energy-dependent phases and is saturable. Sodium chloride delays the onset of the second more rapid energy-dependent phase and decreases the magnitude of gentamicin accumulation for incubations up to 60 minutes at all gentamicin concentrations tested. Simultaneous determinations of accumulation, cell viability, and growth inhibition indicate that antimicrobial activity is correlated with the magnitude of gentamicin accumulation. These observations suggest that altered bacterial accumulation of gentamicin explains the effect of sodium chloride on the antimicrobial activity of gentamicin.
doi_str_mv 10.7164/antibiotics.33.604
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subjects Escherichia coli - drug effects
Escherichia coli - growth & development
Escherichia coli - metabolism
Gentamicins - metabolism
Hydrogen-Ion Concentration
Kinetics
Microbial Sensitivity Tests
Sodium Chloride - pharmacology
title EFFECT OF SODIUM CHLORIDE ON GENTAMICIN ACCUMULATION BY ESCHERICHIA COLI: CORRELATION WITH BACTERIAL GROWTH AND VIABILITY
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