Progesterone-receptor complex in the nuclear fraction of the bovine endometrium
A nuclear exchange assay was utilized to characterize and quantitate the progesterone-receptor complex (PR) of the bovine endometrium. Fifteen Bos taurus cows were observed twice daily for estrus and were assigned to experimental groups at the time of estrus. Endometrium was removed on the day of es...
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| Veröffentlicht in: | Biology of reproduction 1980-09, Vol.23 (2), p.317-323 |
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| container_title | Biology of reproduction |
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| creator | Atkins, D T Kraemer, D C Harms, P G Fleeger, J L |
| description | A nuclear exchange assay was utilized to characterize and quantitate the progesterone-receptor
complex (PR) of the bovine endometrium. Fifteen Bos taurus cows were observed twice daily for
estrus and were assigned to experimental groups at the time of estrus. Endometrium was removed
on the day of estrus, Day 4, Day 10 or 11, and Day 18 of the estrous cycle. Scatchard analysis of
the nuclear exchange assay resulted in a nonlinear fit which could be linearized by the inclusion of
100-fold excess of cortisol. The number of binding sites per endometrial cell from the uterine horn
ipsilateral to the corpus luteum was 34,900 ± 2900 and from the contralateral horn it was 17,000 ±
4400 (P |
| doi_str_mv | 10.1095/biolreprod23.2.317 |
| format | Article |
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complex (PR) of the bovine endometrium. Fifteen Bos taurus cows were observed twice daily for
estrus and were assigned to experimental groups at the time of estrus. Endometrium was removed
on the day of estrus, Day 4, Day 10 or 11, and Day 18 of the estrous cycle. Scatchard analysis of
the nuclear exchange assay resulted in a nonlinear fit which could be linearized by the inclusion of
100-fold excess of cortisol. The number of binding sites per endometrial cell from the uterine horn
ipsilateral to the corpus luteum was 34,900 ± 2900 and from the contralateral horn it was 17,000 ±
4400 (P<0.02). Data on the nuclear PR varied between individuals at estrus, Day 4 and Day 18 of
the cycle.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod23.2.317</identifier><identifier>PMID: 7191340</identifier><language>eng</language><publisher>United States: Society for the Study of Reproduction</publisher><subject>20-alpha-Dihydroprogesterone - pharmacology ; Animals ; Binding Sites ; Cattle ; Cell Nucleus - metabolism ; Corticosterone - pharmacology ; Desoxycorticosterone - pharmacology ; Endometrium - metabolism ; Estrus ; Female ; Pregnancy ; Progesterone - pharmacology ; Protein Binding - drug effects ; Receptors, Progesterone - analysis</subject><ispartof>Biology of reproduction, 1980-09, Vol.23 (2), p.317-323</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7191340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Atkins, D T</creatorcontrib><creatorcontrib>Kraemer, D C</creatorcontrib><creatorcontrib>Harms, P G</creatorcontrib><creatorcontrib>Fleeger, J L</creatorcontrib><creatorcontrib>Ecole Nationale Veterinaire de Lyon, 69 - Charbonnieres-les-Bains (France)</creatorcontrib><title>Progesterone-receptor complex in the nuclear fraction of the bovine endometrium</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>A nuclear exchange assay was utilized to characterize and quantitate the progesterone-receptor
complex (PR) of the bovine endometrium. Fifteen Bos taurus cows were observed twice daily for
estrus and were assigned to experimental groups at the time of estrus. Endometrium was removed
on the day of estrus, Day 4, Day 10 or 11, and Day 18 of the estrous cycle. Scatchard analysis of
the nuclear exchange assay resulted in a nonlinear fit which could be linearized by the inclusion of
100-fold excess of cortisol. The number of binding sites per endometrial cell from the uterine horn
ipsilateral to the corpus luteum was 34,900 ± 2900 and from the contralateral horn it was 17,000 ±
4400 (P<0.02). Data on the nuclear PR varied between individuals at estrus, Day 4 and Day 18 of
the cycle.</description><subject>20-alpha-Dihydroprogesterone - pharmacology</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>Cattle</subject><subject>Cell Nucleus - metabolism</subject><subject>Corticosterone - pharmacology</subject><subject>Desoxycorticosterone - pharmacology</subject><subject>Endometrium - metabolism</subject><subject>Estrus</subject><subject>Female</subject><subject>Pregnancy</subject><subject>Progesterone - pharmacology</subject><subject>Protein Binding - drug effects</subject><subject>Receptors, Progesterone - analysis</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkE1LxDAQhoMo67r6BwSlF711TTJN0xxF_IKFFVzPIW2mu5G2qWlr9d9b3D0NzPvwMvMQcsnoklEl7nLnq4Bt8JbDki-BySMyZ4KrWPI0OyZzSmkaA6RwSs667pNSlgCHGZlJphgkdE7Wb8Fvsesx-AbjgAW2vQ9R4eu2wp_INVG_w6gZigpNiMpgit75JvLl_z73367BCBvra-yDG-pzclKaqsOLw1yQzdPj5uElXq2fXx_uV3HJhepjI3Oe2ARtwXPDmIU0BSmNRJUKCqUCmyUFghFgVKbyXNJUJRTB2qzMMoQFud3XTs9_DdP9unZdgVVlGvRDp6XgUqRcTODVARzyGq1ug6tN-NUHAVN-s893brsbXUDd1aaqJhr0OI4cNNeT14m73nOl8dpsg-v0xztTGaeSCTFp_QNHHnZD</recordid><startdate>198009</startdate><enddate>198009</enddate><creator>Atkins, D T</creator><creator>Kraemer, D C</creator><creator>Harms, P G</creator><creator>Fleeger, J L</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198009</creationdate><title>Progesterone-receptor complex in the nuclear fraction of the bovine endometrium</title><author>Atkins, D T ; Kraemer, D C ; Harms, P G ; Fleeger, J L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f259t-a7b24d4edc2ba11d366377a7e96503f93d84ce3a53a989bb706940e3dd8f88e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>20-alpha-Dihydroprogesterone - pharmacology</topic><topic>Animals</topic><topic>Binding Sites</topic><topic>Cattle</topic><topic>Cell Nucleus - metabolism</topic><topic>Corticosterone - pharmacology</topic><topic>Desoxycorticosterone - pharmacology</topic><topic>Endometrium - metabolism</topic><topic>Estrus</topic><topic>Female</topic><topic>Pregnancy</topic><topic>Progesterone - pharmacology</topic><topic>Protein Binding - drug effects</topic><topic>Receptors, Progesterone - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atkins, D T</creatorcontrib><creatorcontrib>Kraemer, D C</creatorcontrib><creatorcontrib>Harms, P G</creatorcontrib><creatorcontrib>Fleeger, J L</creatorcontrib><creatorcontrib>Ecole Nationale Veterinaire de Lyon, 69 - Charbonnieres-les-Bains (France)</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atkins, D T</au><au>Kraemer, D C</au><au>Harms, P G</au><au>Fleeger, J L</au><aucorp>Ecole Nationale Veterinaire de Lyon, 69 - Charbonnieres-les-Bains (France)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Progesterone-receptor complex in the nuclear fraction of the bovine endometrium</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1980-09</date><risdate>1980</risdate><volume>23</volume><issue>2</issue><spage>317</spage><epage>323</epage><pages>317-323</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>A nuclear exchange assay was utilized to characterize and quantitate the progesterone-receptor
complex (PR) of the bovine endometrium. Fifteen Bos taurus cows were observed twice daily for
estrus and were assigned to experimental groups at the time of estrus. Endometrium was removed
on the day of estrus, Day 4, Day 10 or 11, and Day 18 of the estrous cycle. Scatchard analysis of
the nuclear exchange assay resulted in a nonlinear fit which could be linearized by the inclusion of
100-fold excess of cortisol. The number of binding sites per endometrial cell from the uterine horn
ipsilateral to the corpus luteum was 34,900 ± 2900 and from the contralateral horn it was 17,000 ±
4400 (P<0.02). Data on the nuclear PR varied between individuals at estrus, Day 4 and Day 18 of
the cycle.</abstract><cop>United States</cop><pub>Society for the Study of Reproduction</pub><pmid>7191340</pmid><doi>10.1095/biolreprod23.2.317</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biology of reproduction, 1980-09, Vol.23 (2), p.317-323 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75275625 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 20-alpha-Dihydroprogesterone - pharmacology Animals Binding Sites Cattle Cell Nucleus - metabolism Corticosterone - pharmacology Desoxycorticosterone - pharmacology Endometrium - metabolism Estrus Female Pregnancy Progesterone - pharmacology Protein Binding - drug effects Receptors, Progesterone - analysis |
| title | Progesterone-receptor complex in the nuclear fraction of the bovine endometrium |
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