Influence of homologous blood serum on motility and head-to-head agglutination in nonmotile ejaculated bovine spermatozoa
Twenty-five ejaculates, five from each of five different bulls, were used to determine the influence of heat-treated heifer serum on motility, head-to-head agglutination, and acrosomal integrity in aged nonmotile spermatozoa. Spermatozoa from each ejaculate were diluted immediately after collection...
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| Veröffentlicht in: | Biology of reproduction 1980-09, Vol.23 (2), p.271-275 |
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| creator | Brown, D V Senger, P L |
| description | Twenty-five ejaculates, five from each of five different bulls, were used to determine the
influence of heat-treated heifer serum on motility, head-to-head agglutination, and acrosomal
integrity in aged nonmotile spermatozoa. Spermatozoa from each ejaculate were diluted immediately after collection to 30 x
10 6 spermatozoa/ml in 2.9% sodium citrate and incubated for 8 h at
37°C. As a result of this 8 h incubation, spermatozoal motility was lost and loss of acrosomal
integrity was minimal. Immediately following the 8 h incubation, 20% (v/v) homologous heifer
serum was added to each sample which was again incubated at 37°C for 8 h. Aliquots were evaluated at 1 min, 2, 4, 6, and 8
h for percentage motile spermatozoa (% MOT), percentage intact
acrosomes (PIA), and percentage head-to-head agglutination (HHA). The addition of serum resulted in an immediate (1 min) increase
(P |
| doi_str_mv | 10.1095/biolreprod23.2.271 |
| format | Article |
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influence of heat-treated heifer serum on motility, head-to-head agglutination, and acrosomal
integrity in aged nonmotile spermatozoa. Spermatozoa from each ejaculate were diluted immediately after collection to 30 x
10 6 spermatozoa/ml in 2.9% sodium citrate and incubated for 8 h at
37°C. As a result of this 8 h incubation, spermatozoal motility was lost and loss of acrosomal
integrity was minimal. Immediately following the 8 h incubation, 20% (v/v) homologous heifer
serum was added to each sample which was again incubated at 37°C for 8 h. Aliquots were evaluated at 1 min, 2, 4, 6, and 8
h for percentage motile spermatozoa (% MOT), percentage intact
acrosomes (PIA), and percentage head-to-head agglutination (HHA). The addition of serum resulted in an immediate (1 min) increase
(P<0.01) in progressive motility for all 25 ejaculates. All but
two ejaculates responded to the addition of serum with motility equal to or exceeding the motility
estimate made immediately after collection of the semen. These 23 ejaculates remained motile
throughout the 8 h incubation in serum. No correlation was found between the postcollection
motility estimate in 2.9% sodium citrate and motility immediately (1 min) following addition of
serum. Ejaculates with low postcollection motility responded to the serum treatment as well or
better than ejaculates with high postcollection motility. Significant (P<0.01) HHA occurred in all
ejaculates following the addition of serum. Although % MOT was maximal 1 min following addition of serum, maximum HHA was
not observed until 2 h after serum addition. There was a decline
(P<0.01) in PIA and HHA during the 8 h postserum incubation; however, acrosomal integrity was
higher than HHA at each evaluation time. Once HHA had reached its maximum, an overall positive
correlation (r = 0.73, P<0.01) was found between HHA and PIA for the 2, 4, 6, and 8 h evaluation times.
These data show that ejaculated spermatozoa that have become immotile due to in vitro aging
can regain or exceed their initial motility and undergo HHA following addition of serum.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod23.2.271</identifier><identifier>PMID: 7417671</identifier><language>eng</language><publisher>United States: Society for the Study of Reproduction</publisher><subject>Acrosome ; Animals ; Blood ; Cattle ; Male ; Sperm Agglutination ; Sperm Motility ; Spermatozoa</subject><ispartof>Biology of reproduction, 1980-09, Vol.23 (2), p.271-275</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7417671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, D V</creatorcontrib><creatorcontrib>Senger, P L</creatorcontrib><creatorcontrib>Ecole Nationale Veterinaire de Lyon, 69 - Charbonnieres-les-Bains (France)</creatorcontrib><title>Influence of homologous blood serum on motility and head-to-head agglutination in nonmotile ejaculated bovine spermatozoa</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Twenty-five ejaculates, five from each of five different bulls, were used to determine the
influence of heat-treated heifer serum on motility, head-to-head agglutination, and acrosomal
integrity in aged nonmotile spermatozoa. Spermatozoa from each ejaculate were diluted immediately after collection to 30 x
10 6 spermatozoa/ml in 2.9% sodium citrate and incubated for 8 h at
37°C. As a result of this 8 h incubation, spermatozoal motility was lost and loss of acrosomal
integrity was minimal. Immediately following the 8 h incubation, 20% (v/v) homologous heifer
serum was added to each sample which was again incubated at 37°C for 8 h. Aliquots were evaluated at 1 min, 2, 4, 6, and 8
h for percentage motile spermatozoa (% MOT), percentage intact
acrosomes (PIA), and percentage head-to-head agglutination (HHA). The addition of serum resulted in an immediate (1 min) increase
(P<0.01) in progressive motility for all 25 ejaculates. All but
two ejaculates responded to the addition of serum with motility equal to or exceeding the motility
estimate made immediately after collection of the semen. These 23 ejaculates remained motile
throughout the 8 h incubation in serum. No correlation was found between the postcollection
motility estimate in 2.9% sodium citrate and motility immediately (1 min) following addition of
serum. Ejaculates with low postcollection motility responded to the serum treatment as well or
better than ejaculates with high postcollection motility. Significant (P<0.01) HHA occurred in all
ejaculates following the addition of serum. Although % MOT was maximal 1 min following addition of serum, maximum HHA was
not observed until 2 h after serum addition. There was a decline
(P<0.01) in PIA and HHA during the 8 h postserum incubation; however, acrosomal integrity was
higher than HHA at each evaluation time. Once HHA had reached its maximum, an overall positive
correlation (r = 0.73, P<0.01) was found between HHA and PIA for the 2, 4, 6, and 8 h evaluation times.
These data show that ejaculated spermatozoa that have become immotile due to in vitro aging
can regain or exceed their initial motility and undergo HHA following addition of serum.</description><subject>Acrosome</subject><subject>Animals</subject><subject>Blood</subject><subject>Cattle</subject><subject>Male</subject><subject>Sperm Agglutination</subject><subject>Sperm Motility</subject><subject>Spermatozoa</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNot0EFv1DAQBWCrApVt6R-oBPIFblnscRwnR1QBrVSJA_RsOfE468qxl9hhtfx60u6e3uF9GmkeIbecbTnr5JfepzDjfk4WxBa2oPgF2XAJXaWgad-QDWOsqYRoxDtylfMzY7wWIC7Jpaq5ahTfkONDdGHBOCBNju7SlEIa05JpH1KyNOO8TDRFOqXigy9HaqKlOzS2Kql6SWrGMSzFR1P86nykMcVXjRSfzbAEU9DSPv31EWne4zyZkv4l8568dSZkvDnnNXn6_u333X31-PPHw93Xx8qB7ErVWtu2TkHduQ6GGrBBawxvLQfjXNNyQCVr60TXD3KolXDQ9M4NOPSdbTkT1-Tz6e66058Fc9GTzwOGYCKuj2olQdUS5Ao_nOHST2j1fvaTmY_6vNXafzr1Oz_uDn5GnScTwqqFPhwOIDRoeHUfT86ZpM04-6yffvGuBaa4lFyJ_7bgh3U</recordid><startdate>198009</startdate><enddate>198009</enddate><creator>Brown, D V</creator><creator>Senger, P L</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198009</creationdate><title>Influence of homologous blood serum on motility and head-to-head agglutination in nonmotile ejaculated bovine spermatozoa</title><author>Brown, D V ; Senger, P L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f259t-8dd88f7249f92c42e6edaa18d12aff6812e754df39bc5c473f26bffcecb9d8103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Acrosome</topic><topic>Animals</topic><topic>Blood</topic><topic>Cattle</topic><topic>Male</topic><topic>Sperm Agglutination</topic><topic>Sperm Motility</topic><topic>Spermatozoa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, D V</creatorcontrib><creatorcontrib>Senger, P L</creatorcontrib><creatorcontrib>Ecole Nationale Veterinaire de Lyon, 69 - Charbonnieres-les-Bains (France)</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, D V</au><au>Senger, P L</au><aucorp>Ecole Nationale Veterinaire de Lyon, 69 - Charbonnieres-les-Bains (France)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of homologous blood serum on motility and head-to-head agglutination in nonmotile ejaculated bovine spermatozoa</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1980-09</date><risdate>1980</risdate><volume>23</volume><issue>2</issue><spage>271</spage><epage>275</epage><pages>271-275</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>Twenty-five ejaculates, five from each of five different bulls, were used to determine the
influence of heat-treated heifer serum on motility, head-to-head agglutination, and acrosomal
integrity in aged nonmotile spermatozoa. Spermatozoa from each ejaculate were diluted immediately after collection to 30 x
10 6 spermatozoa/ml in 2.9% sodium citrate and incubated for 8 h at
37°C. As a result of this 8 h incubation, spermatozoal motility was lost and loss of acrosomal
integrity was minimal. Immediately following the 8 h incubation, 20% (v/v) homologous heifer
serum was added to each sample which was again incubated at 37°C for 8 h. Aliquots were evaluated at 1 min, 2, 4, 6, and 8
h for percentage motile spermatozoa (% MOT), percentage intact
acrosomes (PIA), and percentage head-to-head agglutination (HHA). The addition of serum resulted in an immediate (1 min) increase
(P<0.01) in progressive motility for all 25 ejaculates. All but
two ejaculates responded to the addition of serum with motility equal to or exceeding the motility
estimate made immediately after collection of the semen. These 23 ejaculates remained motile
throughout the 8 h incubation in serum. No correlation was found between the postcollection
motility estimate in 2.9% sodium citrate and motility immediately (1 min) following addition of
serum. Ejaculates with low postcollection motility responded to the serum treatment as well or
better than ejaculates with high postcollection motility. Significant (P<0.01) HHA occurred in all
ejaculates following the addition of serum. Although % MOT was maximal 1 min following addition of serum, maximum HHA was
not observed until 2 h after serum addition. There was a decline
(P<0.01) in PIA and HHA during the 8 h postserum incubation; however, acrosomal integrity was
higher than HHA at each evaluation time. Once HHA had reached its maximum, an overall positive
correlation (r = 0.73, P<0.01) was found between HHA and PIA for the 2, 4, 6, and 8 h evaluation times.
These data show that ejaculated spermatozoa that have become immotile due to in vitro aging
can regain or exceed their initial motility and undergo HHA following addition of serum.</abstract><cop>United States</cop><pub>Society for the Study of Reproduction</pub><pmid>7417671</pmid><doi>10.1095/biolreprod23.2.271</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 1980-09, Vol.23 (2), p.271-275 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75274525 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Acrosome Animals Blood Cattle Male Sperm Agglutination Sperm Motility Spermatozoa |
| title | Influence of homologous blood serum on motility and head-to-head agglutination in nonmotile ejaculated bovine spermatozoa |
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