Quantitative assay of phagocytosis by retinal pigment epithelium: an organ culture model
The purpose of the study was to develop a model for quantitative analysis of phagocytosis by retinal pigment epithelium (RPE). The posterior segment of the bovine eye, devoid of the neural retina and vitreous humour, formed a hollowed cup. RPE lining the interior of the cup (RPE cup) was incubated a...
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| Veröffentlicht in: | Experimental eye research 1980-06, Vol.30 (6), p.719-729 |
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| creator | Rosenstock, T. Basu, Ranjana Basu, P.K. Ranadive, N.S. |
| description | The purpose of the study was to develop a model for quantitative analysis of phagocytosis by retinal pigment epithelium (RPE). The posterior segment of the bovine eye, devoid of the neural retina and vitreous humour, formed a hollowed cup. RPE lining the interior of the cup (RPE cup) was incubated as an organ culture at 37°C for 24 hr, with latex particles (1·09 μm) previously coated with
125I-labelled γ-globulin and then thoroughly washed. The amount of radioactivity per RPE cup was related to the phagocytic activity of the RPE cells. Electron microscopy verified that the particles were indeed internalized by RPE. Phagocytosis was significantly inhibited (
P < 0·005) by iodoacetate (10
−3
m), colchicine (10
−4
m) and cytochalasin B (10 μg/ml). Dibutyryl c′AMP (10
−3
m) and bromo-c′GMP (10
−3
m) did not have any significant effect on phagocytosis. |
| doi_str_mv | 10.1016/0014-4835(80)90070-6 |
| format | Article |
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125I-labelled γ-globulin and then thoroughly washed. The amount of radioactivity per RPE cup was related to the phagocytic activity of the RPE cells. Electron microscopy verified that the particles were indeed internalized by RPE. Phagocytosis was significantly inhibited (
P < 0·005) by iodoacetate (10
−3
m), colchicine (10
−4
m) and cytochalasin B (10 μg/ml). Dibutyryl c′AMP (10
−3
m) and bromo-c′GMP (10
−3
m) did not have any significant effect on phagocytosis.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/0014-4835(80)90070-6</identifier><identifier>PMID: 7418749</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Cattle ; latex organ culture ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Models, Biological ; Organ Culture Techniques ; Phagocytosis ; Pigment Epithelium of Eye - physiology ; Pigment Epithelium of Eye - ultrastructure ; retinal pigment epithelium ; Time Factors</subject><ispartof>Experimental eye research, 1980-06, Vol.30 (6), p.719-729</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-da75b395f5ecb257d90c86b25d18e5f1dafc1e8a2db547654a1a1dd2fd0ec6e53</citedby><cites>FETCH-LOGICAL-c357t-da75b395f5ecb257d90c86b25d18e5f1dafc1e8a2db547654a1a1dd2fd0ec6e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4835(80)90070-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7418749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosenstock, T.</creatorcontrib><creatorcontrib>Basu, Ranjana</creatorcontrib><creatorcontrib>Basu, P.K.</creatorcontrib><creatorcontrib>Ranadive, N.S.</creatorcontrib><title>Quantitative assay of phagocytosis by retinal pigment epithelium: an organ culture model</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>The purpose of the study was to develop a model for quantitative analysis of phagocytosis by retinal pigment epithelium (RPE). The posterior segment of the bovine eye, devoid of the neural retina and vitreous humour, formed a hollowed cup. RPE lining the interior of the cup (RPE cup) was incubated as an organ culture at 37°C for 24 hr, with latex particles (1·09 μm) previously coated with
125I-labelled γ-globulin and then thoroughly washed. The amount of radioactivity per RPE cup was related to the phagocytic activity of the RPE cells. Electron microscopy verified that the particles were indeed internalized by RPE. Phagocytosis was significantly inhibited (
P < 0·005) by iodoacetate (10
−3
m), colchicine (10
−4
m) and cytochalasin B (10 μg/ml). Dibutyryl c′AMP (10
−3
m) and bromo-c′GMP (10
−3
m) did not have any significant effect on phagocytosis.</description><subject>Animals</subject><subject>Cattle</subject><subject>latex organ culture</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Models, Biological</subject><subject>Organ Culture Techniques</subject><subject>Phagocytosis</subject><subject>Pigment Epithelium of Eye - physiology</subject><subject>Pigment Epithelium of Eye - ultrastructure</subject><subject>retinal pigment epithelium</subject><subject>Time Factors</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQx4Mo6_r4Bgo5iR6qSZs0rQdBxBcIIih4C2ky3Y20TU1Sod_errvs0cs8_zPD_BA6oeSSEppfEUJZwoqMnxfkoiREkCTfQXNKyjwhU7qL5lvJPjoI4WuqZkywGZoJRgvByjn6fBtUF21U0f4AViGoEbsa90u1cHqMLtiAqxF7iLZTDe7tooUuYuhtXEJjh_Yaqw47v5isHpo4eMCtM9Acob1aNQGON_4QfTzcv989JS-vj893ty-JzriIiVGCV1nJaw66SrkwJdFFPkWGFsBralStKRQqNRVnIudMUUWNSWtDQOfAs0N0tt7be_c9QIiytUFD06gO3BCk4KmgRUomIVsLtXcheKhl722r_CgpkSugckVLrmjJgsg_oDKfxk43-4eqBbMd2hCc-jfrPkxP_ljwMmgLnQZjPegojbP_H_gF1T2Gug</recordid><startdate>198006</startdate><enddate>198006</enddate><creator>Rosenstock, T.</creator><creator>Basu, Ranjana</creator><creator>Basu, P.K.</creator><creator>Ranadive, N.S.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198006</creationdate><title>Quantitative assay of phagocytosis by retinal pigment epithelium: an organ culture model</title><author>Rosenstock, T. ; Basu, Ranjana ; Basu, P.K. ; Ranadive, N.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-da75b395f5ecb257d90c86b25d18e5f1dafc1e8a2db547654a1a1dd2fd0ec6e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>latex organ culture</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Electron, Scanning</topic><topic>Models, Biological</topic><topic>Organ Culture Techniques</topic><topic>Phagocytosis</topic><topic>Pigment Epithelium of Eye - physiology</topic><topic>Pigment Epithelium of Eye - ultrastructure</topic><topic>retinal pigment epithelium</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosenstock, T.</creatorcontrib><creatorcontrib>Basu, Ranjana</creatorcontrib><creatorcontrib>Basu, P.K.</creatorcontrib><creatorcontrib>Ranadive, N.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosenstock, T.</au><au>Basu, Ranjana</au><au>Basu, P.K.</au><au>Ranadive, N.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative assay of phagocytosis by retinal pigment epithelium: an organ culture model</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>1980-06</date><risdate>1980</risdate><volume>30</volume><issue>6</issue><spage>719</spage><epage>729</epage><pages>719-729</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><abstract>The purpose of the study was to develop a model for quantitative analysis of phagocytosis by retinal pigment epithelium (RPE). The posterior segment of the bovine eye, devoid of the neural retina and vitreous humour, formed a hollowed cup. RPE lining the interior of the cup (RPE cup) was incubated as an organ culture at 37°C for 24 hr, with latex particles (1·09 μm) previously coated with
125I-labelled γ-globulin and then thoroughly washed. The amount of radioactivity per RPE cup was related to the phagocytic activity of the RPE cells. Electron microscopy verified that the particles were indeed internalized by RPE. Phagocytosis was significantly inhibited (
P < 0·005) by iodoacetate (10
−3
m), colchicine (10
−4
m) and cytochalasin B (10 μg/ml). Dibutyryl c′AMP (10
−3
m) and bromo-c′GMP (10
−3
m) did not have any significant effect on phagocytosis.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>7418749</pmid><doi>10.1016/0014-4835(80)90070-6</doi><tpages>11</tpages></addata></record> |
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| ispartof | Experimental eye research, 1980-06, Vol.30 (6), p.719-729 |
| issn | 0014-4835 1096-0007 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75271820 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cattle latex organ culture Microscopy, Electron Microscopy, Electron, Scanning Models, Biological Organ Culture Techniques Phagocytosis Pigment Epithelium of Eye - physiology Pigment Epithelium of Eye - ultrastructure retinal pigment epithelium Time Factors |
| title | Quantitative assay of phagocytosis by retinal pigment epithelium: an organ culture model |
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