Synthesis of Progesterone and Estradiol by Monkey Luteal Cells in Culture: Effects of Insulin, Thyroxine, Cortisol, and Cholesterol With and Without hCG
The objective of the present work was to define further the hormonal factor or factors which participate in the maintenance of primate luteal cells in culture, including both luteotropic and luteolytic processes. Corpora lutea (CL) were obtained from rhesus monkeys during midluteal phase (17-19 days...
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| Veröffentlicht in: | Biology of reproduction 1980-08, Vol.23 (1), p.21-28 |
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| creator | Gulyas, B J Yuan, L C Hodgen, G D |
| description | The objective of the present work was to define further the hormonal factor or factors which
participate in the maintenance of primate luteal cells in culture, including both luteotropic and
luteolytic processes. Corpora lutea (CL) were obtained from rhesus monkeys during midluteal
phase (17-19 days after onset of menses) of the menstrual cycle and were dissociated with collagenase. In the first experiment
the basic culture medium (F10 + 10% fetal calf serum) was supplemented by the nongonadotropin hormones insulin (I), thyroxin
(T 4 ), and cortisol (C), and/or
cholesterol (Ch), in the presence or absence of human chorinoic gonadotropin (hCG). Concentrations of progesterone (P) and
estradiol (E 2 ) were determined by radioimmunoassay of unextracted
samples. P synthesis by luteal cells in the basic medium progressively declined throughout the
10-day culture period. Although we were unable to stabilize P production under any condition,
with the addition of I-T 4 -C and in the presence of hCG, P synthesis was significantly higher
(P |
| doi_str_mv | 10.1095/biolreprod23.1.21 |
| format | Article |
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participate in the maintenance of primate luteal cells in culture, including both luteotropic and
luteolytic processes. Corpora lutea (CL) were obtained from rhesus monkeys during midluteal
phase (17-19 days after onset of menses) of the menstrual cycle and were dissociated with collagenase. In the first experiment
the basic culture medium (F10 + 10% fetal calf serum) was supplemented by the nongonadotropin hormones insulin (I), thyroxin
(T 4 ), and cortisol (C), and/or
cholesterol (Ch), in the presence or absence of human chorinoic gonadotropin (hCG). Concentrations of progesterone (P) and
estradiol (E 2 ) were determined by radioimmunoassay of unextracted
samples. P synthesis by luteal cells in the basic medium progressively declined throughout the
10-day culture period. Although we were unable to stabilize P production under any condition,
with the addition of I-T 4 -C and in the presence of hCG, P synthesis was significantly higher
(P <0.05) throughout the 10-day incubation period. E 2 production showed a similar declining
pattern, regardless of the composition of the culture medium. Ch alone had no beneficial effects on
maintenance of high levels of P secretion, whereas in the presence of hCG production of P was
enhanced (P<0.01) for the first 2 days of culture. In the second experiment the combination of
any two nongonadotropin hormones with hCG appeared equally beneficial during the first 2 days
of culture, after which P secretion declined rapidly. In an additional experiment, luteal cells were
initially cultured with I-T 4 -C for 2, 4, 6 or 8 days, at which times hCG was added for the remaining
days of culture. When hCG was initially added on Days 2 or 4, P production was significantly
elevated on Days 4 and 6, and on Day 6, respectively, followed by a gradual decline. The results
demonstrate that the combination of nongonadotropin hormones, when in concert with hCG, is
beneficial in maintaining P synthesis at higher levels for longer intervals. Addition of hCG slowed
the declining pattern of P production when introduced on culture Days 2 or 4; nevertheless, the
declining pattern of P output continued. The current state of this culture system does not permit
in vitro imitation of chorionic gonadotropin-induced "rescue" of CL, as it occurs in the fertile
menstrual cycle.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod23.1.21</identifier><identifier>PMID: 6998519</identifier><language>eng</language><publisher>United States: Society for the Study of Reproduction</publisher><subject>Animals ; Cells, Cultured ; Cholesterol - pharmacology ; Chorionic Gonadotropin - pharmacology ; Corpus Luteum - drug effects ; Estradiol - biosynthesis ; Female ; Hydrocortisone - pharmacology ; Insulin - pharmacology ; Macaca mulatta ; Progesterone - biosynthesis ; Thyroxine - pharmacology</subject><ispartof>Biology of reproduction, 1980-08, Vol.23 (1), p.21-28</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c325t-5261464e21746cf6f4e6786ce187c82894a30736140326d86329bd04c910362b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6998519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gulyas, B J</creatorcontrib><creatorcontrib>Yuan, L C</creatorcontrib><creatorcontrib>Hodgen, G D</creatorcontrib><title>Synthesis of Progesterone and Estradiol by Monkey Luteal Cells in Culture: Effects of Insulin, Thyroxine, Cortisol, and Cholesterol With and Without hCG</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The objective of the present work was to define further the hormonal factor or factors which
participate in the maintenance of primate luteal cells in culture, including both luteotropic and
luteolytic processes. Corpora lutea (CL) were obtained from rhesus monkeys during midluteal
phase (17-19 days after onset of menses) of the menstrual cycle and were dissociated with collagenase. In the first experiment
the basic culture medium (F10 + 10% fetal calf serum) was supplemented by the nongonadotropin hormones insulin (I), thyroxin
(T 4 ), and cortisol (C), and/or
cholesterol (Ch), in the presence or absence of human chorinoic gonadotropin (hCG). Concentrations of progesterone (P) and
estradiol (E 2 ) were determined by radioimmunoassay of unextracted
samples. P synthesis by luteal cells in the basic medium progressively declined throughout the
10-day culture period. Although we were unable to stabilize P production under any condition,
with the addition of I-T 4 -C and in the presence of hCG, P synthesis was significantly higher
(P <0.05) throughout the 10-day incubation period. E 2 production showed a similar declining
pattern, regardless of the composition of the culture medium. Ch alone had no beneficial effects on
maintenance of high levels of P secretion, whereas in the presence of hCG production of P was
enhanced (P<0.01) for the first 2 days of culture. In the second experiment the combination of
any two nongonadotropin hormones with hCG appeared equally beneficial during the first 2 days
of culture, after which P secretion declined rapidly. In an additional experiment, luteal cells were
initially cultured with I-T 4 -C for 2, 4, 6 or 8 days, at which times hCG was added for the remaining
days of culture. When hCG was initially added on Days 2 or 4, P production was significantly
elevated on Days 4 and 6, and on Day 6, respectively, followed by a gradual decline. The results
demonstrate that the combination of nongonadotropin hormones, when in concert with hCG, is
beneficial in maintaining P synthesis at higher levels for longer intervals. Addition of hCG slowed
the declining pattern of P production when introduced on culture Days 2 or 4; nevertheless, the
declining pattern of P output continued. The current state of this culture system does not permit
in vitro imitation of chorionic gonadotropin-induced "rescue" of CL, as it occurs in the fertile
menstrual cycle.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cholesterol - pharmacology</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Corpus Luteum - drug effects</subject><subject>Estradiol - biosynthesis</subject><subject>Female</subject><subject>Hydrocortisone - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>Macaca mulatta</subject><subject>Progesterone - biosynthesis</subject><subject>Thyroxine - pharmacology</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkdtO3DAQhi1URLfAA3CB5AvUq83iQ-IkvauiLUVaVCRAXFo5TIhbrw22o23epI9b76GoV2PN_PP94xmELihZUFJm142y2sGrsx3jC7pg9AjNaMbKJGei-IBmhBCRcC74R_TJ-5-E0JQzfoJORFkWGS1n6M_DZMIAXnlse3zv7Av4AM4awLXp8NIHV3fRBTcTvrPmF0x4NQaoNa5Aa4-VwdWow-jgC172PbRhB7o1ftTKzPHjMDn7WxmY48q6oLzV8x25GqzeW2n8rMKwS24fdgx4qG7O0HFfaw_nh3iKnr4tH6vvyerHzW31dZW0nGUhyZigqUiB0TwVbS_6FEReiBZokbcFK8q05iTnUUQ4E10hOCubjqRtSQkXrOGn6POeG7f4NsaJ5Fr5Nv6tNmBHL_PoILKcRyHdC1tnvXfQy1en1rWbJCVyew35_zUklYzGnssDfGzW0L13HNYf61f7-qBeho1yIP261jqqudxsNv8wfwHbr5Wi</recordid><startdate>198008</startdate><enddate>198008</enddate><creator>Gulyas, B J</creator><creator>Yuan, L C</creator><creator>Hodgen, G D</creator><general>Society for the Study of Reproduction</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198008</creationdate><title>Synthesis of Progesterone and Estradiol by Monkey Luteal Cells in Culture: Effects of Insulin, Thyroxine, Cortisol, and Cholesterol With and Without hCG</title><author>Gulyas, B J ; Yuan, L C ; Hodgen, G D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-5261464e21746cf6f4e6786ce187c82894a30736140326d86329bd04c910362b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Cholesterol - pharmacology</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>Corpus Luteum - drug effects</topic><topic>Estradiol - biosynthesis</topic><topic>Female</topic><topic>Hydrocortisone - pharmacology</topic><topic>Insulin - pharmacology</topic><topic>Macaca mulatta</topic><topic>Progesterone - biosynthesis</topic><topic>Thyroxine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gulyas, B J</creatorcontrib><creatorcontrib>Yuan, L C</creatorcontrib><creatorcontrib>Hodgen, G D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gulyas, B J</au><au>Yuan, L C</au><au>Hodgen, G D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of Progesterone and Estradiol by Monkey Luteal Cells in Culture: Effects of Insulin, Thyroxine, Cortisol, and Cholesterol With and Without hCG</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1980-08</date><risdate>1980</risdate><volume>23</volume><issue>1</issue><spage>21</spage><epage>28</epage><pages>21-28</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>The objective of the present work was to define further the hormonal factor or factors which
participate in the maintenance of primate luteal cells in culture, including both luteotropic and
luteolytic processes. Corpora lutea (CL) were obtained from rhesus monkeys during midluteal
phase (17-19 days after onset of menses) of the menstrual cycle and were dissociated with collagenase. In the first experiment
the basic culture medium (F10 + 10% fetal calf serum) was supplemented by the nongonadotropin hormones insulin (I), thyroxin
(T 4 ), and cortisol (C), and/or
cholesterol (Ch), in the presence or absence of human chorinoic gonadotropin (hCG). Concentrations of progesterone (P) and
estradiol (E 2 ) were determined by radioimmunoassay of unextracted
samples. P synthesis by luteal cells in the basic medium progressively declined throughout the
10-day culture period. Although we were unable to stabilize P production under any condition,
with the addition of I-T 4 -C and in the presence of hCG, P synthesis was significantly higher
(P <0.05) throughout the 10-day incubation period. E 2 production showed a similar declining
pattern, regardless of the composition of the culture medium. Ch alone had no beneficial effects on
maintenance of high levels of P secretion, whereas in the presence of hCG production of P was
enhanced (P<0.01) for the first 2 days of culture. In the second experiment the combination of
any two nongonadotropin hormones with hCG appeared equally beneficial during the first 2 days
of culture, after which P secretion declined rapidly. In an additional experiment, luteal cells were
initially cultured with I-T 4 -C for 2, 4, 6 or 8 days, at which times hCG was added for the remaining
days of culture. When hCG was initially added on Days 2 or 4, P production was significantly
elevated on Days 4 and 6, and on Day 6, respectively, followed by a gradual decline. The results
demonstrate that the combination of nongonadotropin hormones, when in concert with hCG, is
beneficial in maintaining P synthesis at higher levels for longer intervals. Addition of hCG slowed
the declining pattern of P production when introduced on culture Days 2 or 4; nevertheless, the
declining pattern of P output continued. The current state of this culture system does not permit
in vitro imitation of chorionic gonadotropin-induced "rescue" of CL, as it occurs in the fertile
menstrual cycle.</abstract><cop>United States</cop><pub>Society for the Study of Reproduction</pub><pmid>6998519</pmid><doi>10.1095/biolreprod23.1.21</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biology of reproduction, 1980-08, Vol.23 (1), p.21-28 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75266573 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Cells, Cultured Cholesterol - pharmacology Chorionic Gonadotropin - pharmacology Corpus Luteum - drug effects Estradiol - biosynthesis Female Hydrocortisone - pharmacology Insulin - pharmacology Macaca mulatta Progesterone - biosynthesis Thyroxine - pharmacology |
| title | Synthesis of Progesterone and Estradiol by Monkey Luteal Cells in Culture: Effects of Insulin, Thyroxine, Cortisol, and Cholesterol With and Without hCG |
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