Promoter for the Establishment of Repressor Synthesis in Bacteriophage λ
Transcription of the λ repressor gene (cI) is positively regulated by the phage-encoded proteins cII and cIII. We have isolated and characterized the 5′-terminal region of this RNA and shown that it originates at a promoter (pE) located between genes cro and cII. The DNA sequence of this promoter sh...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1980-06, Vol.77 (6), p.3191-3195 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Schmeissner, Ursula Court, Donald Shimatake, Hiroyuki Rosenberg, Martin |
| description | Transcription of the λ repressor gene (cI) is positively regulated by the phage-encoded proteins cII and cIII. We have isolated and characterized the 5′-terminal region of this RNA and shown that it originates at a promoter (pE) located between genes cro and cII. The DNA sequence of this promoter shows little homology to other known promoters. Initiation of transcription from pEis abolished by the cis-dominant mutations cY; these mutations alter the ``-10'' and ``-35'' regions of the promoter. We propose that the ``-35'' region is the site of activation of pE, possibly via the direct interaction of protein cII. |
| doi_str_mv | 10.1073/pnas.77.6.3191 |
| format | Article |
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We have isolated and characterized the 5′-terminal region of this RNA and shown that it originates at a promoter (pE) located between genes cro and cII. The DNA sequence of this promoter shows little homology to other known promoters. Initiation of transcription from pEis abolished by the cis-dominant mutations cY; these mutations alter the ``-10'' and ``-35'' regions of the promoter. We propose that the ``-35'' region is the site of activation of pE, possibly via the direct interaction of protein cII.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.77.6.3191</identifier><identifier>PMID: 6447872</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Bacteriophage lambda - genetics ; Bacteriophages ; Base Sequence ; Binding Sites ; Chromosome Mapping ; Cloning, Molecular ; DNA ; Escherichia coli - genetics ; Genes ; Genes, Regulator ; Genetic mutation ; Infections ; Mutation ; Nucleotide sequences ; Nucleotides ; Oligonucleotides ; Operon ; Plasmids ; Promoter regions ; Repressor Proteins - biosynthesis ; Repressor Proteins - genetics ; RNA ; RNA Polymerase I - metabolism ; RNA, Messenger - isolation & purification ; Transcription Factors - genetics ; Transcription, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1980-06, Vol.77 (6), p.3191-3195</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3021-b412e297ae4afcb0eba4c5bd7c012a5c072162fde386bd94e6bc58855efa21fe3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/77/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/8853$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/8853$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,725,778,782,801,883,27911,27912,53778,53780,58004,58237</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6447872$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmeissner, Ursula</creatorcontrib><creatorcontrib>Court, Donald</creatorcontrib><creatorcontrib>Shimatake, Hiroyuki</creatorcontrib><creatorcontrib>Rosenberg, Martin</creatorcontrib><title>Promoter for the Establishment of Repressor Synthesis in Bacteriophage λ</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Transcription of the λ repressor gene (cI) is positively regulated by the phage-encoded proteins cII and cIII. We have isolated and characterized the 5′-terminal region of this RNA and shown that it originates at a promoter (pE) located between genes cro and cII. The DNA sequence of this promoter shows little homology to other known promoters. Initiation of transcription from pEis abolished by the cis-dominant mutations cY; these mutations alter the ``-10'' and ``-35'' regions of the promoter. We propose that the ``-35'' region is the site of activation of pE, possibly via the direct interaction of protein cII.</description><subject>Bacteriophage lambda - genetics</subject><subject>Bacteriophages</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>Escherichia coli - genetics</subject><subject>Genes</subject><subject>Genes, Regulator</subject><subject>Genetic mutation</subject><subject>Infections</subject><subject>Mutation</subject><subject>Nucleotide sequences</subject><subject>Nucleotides</subject><subject>Oligonucleotides</subject><subject>Operon</subject><subject>Plasmids</subject><subject>Promoter regions</subject><subject>Repressor Proteins - biosynthesis</subject><subject>Repressor Proteins - genetics</subject><subject>RNA</subject><subject>RNA Polymerase I - metabolism</subject><subject>RNA, Messenger - isolation & purification</subject><subject>Transcription Factors - genetics</subject><subject>Transcription, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMtKxDAUhoMo43jZCi6Erty1JmnStAsXKt5gQPGyDmnmxKm0TU06os_mO_hMpsw4jOAqi__7zjn5ETogOCFYpCddq3wiRJIlKSnIBhoTXJA4YwXeRGOMqYhzRtk22vH-FWNc8ByP0ChjTOSCjtHtvbON7cFFxrqon0F06XtV1pWfNdD2kTXRA3QOvA_x42cbCF_5qGqjc6WDVtlupl4g-v7aQ1tG1R72l-8uer66fLq4iSd317cXZ5NYp5iSuGSEAi2EAqaMLjGUimleToXGhCqusaAko2YKaZ6V04JBVmqe55yDUZQYSHfR6WJuNy8bmOpwpVO17FzVKPcprark36StZvLFvsuUDZ8P_vHSd_ZtDr6XTeU11LVqwc69FJxyTjgLYLIAtbPeOzCrHQTLoXs5dC-FkJkcug_C0fplK3xZ9trmwftNV74087ru4aNfG_QvGPLDRf7qe-tWQGgpTX8AOiijhA</recordid><startdate>19800601</startdate><enddate>19800601</enddate><creator>Schmeissner, Ursula</creator><creator>Court, Donald</creator><creator>Shimatake, Hiroyuki</creator><creator>Rosenberg, Martin</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19800601</creationdate><title>Promoter for the Establishment of Repressor Synthesis in Bacteriophage λ</title><author>Schmeissner, Ursula ; Court, Donald ; Shimatake, Hiroyuki ; Rosenberg, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3021-b412e297ae4afcb0eba4c5bd7c012a5c072162fde386bd94e6bc58855efa21fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Bacteriophage lambda - genetics</topic><topic>Bacteriophages</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>Escherichia coli - genetics</topic><topic>Genes</topic><topic>Genes, Regulator</topic><topic>Genetic mutation</topic><topic>Infections</topic><topic>Mutation</topic><topic>Nucleotide sequences</topic><topic>Nucleotides</topic><topic>Oligonucleotides</topic><topic>Operon</topic><topic>Plasmids</topic><topic>Promoter regions</topic><topic>Repressor Proteins - biosynthesis</topic><topic>Repressor Proteins - genetics</topic><topic>RNA</topic><topic>RNA Polymerase I - metabolism</topic><topic>RNA, Messenger - isolation & purification</topic><topic>Transcription Factors - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmeissner, Ursula</creatorcontrib><creatorcontrib>Court, Donald</creatorcontrib><creatorcontrib>Shimatake, Hiroyuki</creatorcontrib><creatorcontrib>Rosenberg, Martin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmeissner, Ursula</au><au>Court, Donald</au><au>Shimatake, Hiroyuki</au><au>Rosenberg, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promoter for the Establishment of Repressor Synthesis in Bacteriophage λ</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1980-06-01</date><risdate>1980</risdate><volume>77</volume><issue>6</issue><spage>3191</spage><epage>3195</epage><pages>3191-3195</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Transcription of the λ repressor gene (cI) is positively regulated by the phage-encoded proteins cII and cIII. We have isolated and characterized the 5′-terminal region of this RNA and shown that it originates at a promoter (pE) located between genes cro and cII. The DNA sequence of this promoter shows little homology to other known promoters. Initiation of transcription from pEis abolished by the cis-dominant mutations cY; these mutations alter the ``-10'' and ``-35'' regions of the promoter. We propose that the ``-35'' region is the site of activation of pE, possibly via the direct interaction of protein cII.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6447872</pmid><doi>10.1073/pnas.77.6.3191</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1980-06, Vol.77 (6), p.3191-3195 |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Bacteriophage lambda - genetics Bacteriophages Base Sequence Binding Sites Chromosome Mapping Cloning, Molecular DNA Escherichia coli - genetics Genes Genes, Regulator Genetic mutation Infections Mutation Nucleotide sequences Nucleotides Oligonucleotides Operon Plasmids Promoter regions Repressor Proteins - biosynthesis Repressor Proteins - genetics RNA RNA Polymerase I - metabolism RNA, Messenger - isolation & purification Transcription Factors - genetics Transcription, Genetic |
| title | Promoter for the Establishment of Repressor Synthesis in Bacteriophage λ |
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