Expression of a Bacterial Gene in Mammalian Cells
Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme....
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1980-09, Vol.209 (4463), p.1422-1427 |
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| container_issue | 4463 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Mulligan, R. C. Berg, P. |
| description | Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells. |
| doi_str_mv | 10.1126/science.6251549 |
| format | Article |
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Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.6251549</identifier><identifier>PMID: 6251549</identifier><language>eng</language><publisher>United States: The American Association for the Advancement of Science</publisher><subject>Animal cells ; Animals ; Bacterial genetics ; Cells ; Cells, Cultured ; Cloning, Molecular - methods ; DNA ; DNA, Bacterial - genetics ; DNA, Recombinant ; Escherichia coli ; Expression of Cloned Genes in New Environment ; Gene expression ; Genes ; Genetic vectors ; Genomes ; Haplorhini ; Humans ; Hypoxanthine Phosphoribosyltransferase - genetics ; Kidney cells ; Lesch-Nyhan Syndrome - genetics ; Messenger RNA ; Pentosyltransferases - genetics ; Simian virus 40 - genetics ; Transduction, Genetic ; Transfection ; Transformation, Genetic ; Viruses</subject><ispartof>Science (American Association for the Advancement of Science), 1980-09, Vol.209 (4463), p.1422-1427</ispartof><rights>Copyright 1980 The American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-c54786c1e136973e0e6a023c7f209ae725de88a232556b31dfd428092197cdeb3</citedby><cites>FETCH-LOGICAL-c446t-c54786c1e136973e0e6a023c7f209ae725de88a232556b31dfd428092197cdeb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1685206$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1685206$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2875,2876,27915,27916,58008,58241</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6251549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mulligan, R. C.</creatorcontrib><creatorcontrib>Berg, P.</creatorcontrib><title>Expression of a Bacterial Gene in Mammalian Cells</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Bacterial genetics</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular - methods</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Recombinant</subject><subject>Escherichia coli</subject><subject>Expression of Cloned Genes in New Environment</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic vectors</subject><subject>Genomes</subject><subject>Haplorhini</subject><subject>Humans</subject><subject>Hypoxanthine Phosphoribosyltransferase - genetics</subject><subject>Kidney cells</subject><subject>Lesch-Nyhan Syndrome - genetics</subject><subject>Messenger RNA</subject><subject>Pentosyltransferases - genetics</subject><subject>Simian virus 40 - genetics</subject><subject>Transduction, Genetic</subject><subject>Transfection</subject><subject>Transformation, Genetic</subject><subject>Viruses</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkL1PwzAUxC0EKqUws4CUiQWl9UfsxGOJSkEq6gKz5TovlavELnEqlf8eo0Z0esP93unuELoneEoIFbNgLDgDU0E54Zm8QGOCJU8lxewSjTFmIi1wzq_RTQg7jKMm2QiNBnyMyOK47yAE613i60QnL9r00FndJEtwkFiXfOi21Y3VLimhacItuqp1E-BuuBP09br4LN_S1Xr5Xs5Xqcky0aeGZ3khDAHChMwZYBAaU2bymmKpIae8gqLQlFHOxYaRqq4yWmBJicxNBRs2QU8n333nvw8QetXaYGIC7cAfgsp57EBoEcHnE7jVDSjrjHc9HHvjmwa2oGKocq3mRMhYnkZ6dqJN50PooFb7zra6-1EEq79J1TCpGjaKH49DkMOmheqfP-sPJ30Xet-d7UTBKRbsFzzDeZQ</recordid><startdate>19800919</startdate><enddate>19800919</enddate><creator>Mulligan, R. C.</creator><creator>Berg, P.</creator><general>The American Association for the Advancement of Science</general><general>American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800919</creationdate><title>Expression of a Bacterial Gene in Mammalian Cells</title><author>Mulligan, R. C. ; Berg, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-c54786c1e136973e0e6a023c7f209ae725de88a232556b31dfd428092197cdeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Bacterial genetics</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular - methods</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Recombinant</topic><topic>Escherichia coli</topic><topic>Expression of Cloned Genes in New Environment</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic vectors</topic><topic>Genomes</topic><topic>Haplorhini</topic><topic>Humans</topic><topic>Hypoxanthine Phosphoribosyltransferase - genetics</topic><topic>Kidney cells</topic><topic>Lesch-Nyhan Syndrome - genetics</topic><topic>Messenger RNA</topic><topic>Pentosyltransferases - genetics</topic><topic>Simian virus 40 - genetics</topic><topic>Transduction, Genetic</topic><topic>Transfection</topic><topic>Transformation, Genetic</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mulligan, R. 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C.</au><au>Berg, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a Bacterial Gene in Mammalian Cells</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1980-09-19</date><risdate>1980</risdate><volume>209</volume><issue>4463</issue><spage>1422</spage><epage>1427</epage><pages>1422-1427</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.</abstract><cop>United States</cop><pub>The American Association for the Advancement of Science</pub><pmid>6251549</pmid><doi>10.1126/science.6251549</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1980-09, Vol.209 (4463), p.1422-1427 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75251128 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Animal cells Animals Bacterial genetics Cells Cells, Cultured Cloning, Molecular - methods DNA DNA, Bacterial - genetics DNA, Recombinant Escherichia coli Expression of Cloned Genes in New Environment Gene expression Genes Genetic vectors Genomes Haplorhini Humans Hypoxanthine Phosphoribosyltransferase - genetics Kidney cells Lesch-Nyhan Syndrome - genetics Messenger RNA Pentosyltransferases - genetics Simian virus 40 - genetics Transduction, Genetic Transfection Transformation, Genetic Viruses |
| title | Expression of a Bacterial Gene in Mammalian Cells |
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