Mechanism of microtubule depolymerization. Correlation of rapid induced disassembly experiments with a kinetic model for endwise depolymerization
Microtubule disassembly has been studied using a rapid dilution technique (Karr, T.L., and Purich, D.L. (1979) J. Biol. Chem. 254, 10885-10888). Disassembly curves, generated by computer from the solution of series first order differential equations (see following paper), were fit to experimental da...
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| Veröffentlicht in: | The Journal of biological chemistry 1980-09, Vol.255 (18), p.8560-8566 |
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| container_title | The Journal of biological chemistry |
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| creator | Karr, T L Kristofferson, D Purich, D L |
| description | Microtubule disassembly has been studied using a rapid dilution technique (Karr, T.L., and Purich, D.L. (1979) J. Biol. Chem.
254, 10885-10888). Disassembly curves, generated by computer from the solution of series first order differential equations
(see following paper), were fit to experimental data with excellent agreement when the diluted microtubules contained no microtubule-associated
proteins. The rate constant for dimer release from microtubules was found to be 154 s-1. Assuming a critical tubulin concentration
of 8 to 9 x 10(-6) M, the apparent bimolecular rate constant (2 x 10(7) M-1 s-1) for assembly is near the diffusion limit.
It was also possible to use the rapid dilution technique for quantitatively correlating the disassembly rate to the number
concentration of microtubule ends. These findings suggest that the dynamics of tubulin interactions with microtubules may
be characterized in terms of an endwise depolymerization model. A re-evaluation of cold induced depolymerization kinetics
(see miniprint supplement) is also fully consistent with our analysis of disassembly dynamics. |
| doi_str_mv | 10.1016/S0021-9258(18)43534-X |
| format | Article |
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254, 10885-10888). Disassembly curves, generated by computer from the solution of series first order differential equations
(see following paper), were fit to experimental data with excellent agreement when the diluted microtubules contained no microtubule-associated
proteins. The rate constant for dimer release from microtubules was found to be 154 s-1. Assuming a critical tubulin concentration
of 8 to 9 x 10(-6) M, the apparent bimolecular rate constant (2 x 10(7) M-1 s-1) for assembly is near the diffusion limit.
It was also possible to use the rapid dilution technique for quantitatively correlating the disassembly rate to the number
concentration of microtubule ends. These findings suggest that the dynamics of tubulin interactions with microtubules may
be characterized in terms of an endwise depolymerization model. A re-evaluation of cold induced depolymerization kinetics
(see miniprint supplement) is also fully consistent with our analysis of disassembly dynamics.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)43534-X</identifier><identifier>PMID: 7410377</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Kinetics ; Macromolecular Substances ; Methods ; Microtubules - ultrastructure ; Tubulin - metabolism</subject><ispartof>The Journal of biological chemistry, 1980-09, Vol.255 (18), p.8560-8566</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-581b7dc2b29349baf6c41ddbf0a466ad6757486391d0584645a247595a02703d3</citedby><cites>FETCH-LOGICAL-c431t-581b7dc2b29349baf6c41ddbf0a466ad6757486391d0584645a247595a02703d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7410377$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karr, T L</creatorcontrib><creatorcontrib>Kristofferson, D</creatorcontrib><creatorcontrib>Purich, D L</creatorcontrib><title>Mechanism of microtubule depolymerization. Correlation of rapid induced disassembly experiments with a kinetic model for endwise depolymerization</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Microtubule disassembly has been studied using a rapid dilution technique (Karr, T.L., and Purich, D.L. (1979) J. Biol. Chem.
254, 10885-10888). Disassembly curves, generated by computer from the solution of series first order differential equations
(see following paper), were fit to experimental data with excellent agreement when the diluted microtubules contained no microtubule-associated
proteins. The rate constant for dimer release from microtubules was found to be 154 s-1. Assuming a critical tubulin concentration
of 8 to 9 x 10(-6) M, the apparent bimolecular rate constant (2 x 10(7) M-1 s-1) for assembly is near the diffusion limit.
It was also possible to use the rapid dilution technique for quantitatively correlating the disassembly rate to the number
concentration of microtubule ends. These findings suggest that the dynamics of tubulin interactions with microtubules may
be characterized in terms of an endwise depolymerization model. A re-evaluation of cold induced depolymerization kinetics
(see miniprint supplement) is also fully consistent with our analysis of disassembly dynamics.</description><subject>Kinetics</subject><subject>Macromolecular Substances</subject><subject>Methods</subject><subject>Microtubules - ultrastructure</subject><subject>Tubulin - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplUU1v1DAQtSpQWQo_oZIlJASHFDu2Y-eIVkArFfVAK-3NcuxJY4jjYCdatv-Cf0z2Q70wl9Fo3puneQ-hS0quKKHVpx-ElLSoS6E-UPWRM8F4sTlDK0oUK5igmxdo9Qx5hV7n_JMsxWt6js4lp4RJuUJ_v4PtzOBzwLHFwdsUp7mZe8AOxtjvAiT_ZCYfhyu8jilBfxj24GRG77Af3GzBYeezyRlC0-8w_BkXWoBhynjrpw4b_MsPMHmLQ3TQ4zYmDIPb-vy_zhv0sjV9hrenfoEevn65X18Xt3ffbtafbwvLGZ0KoWgjnS2bsma8bkxbWU6da1pieFUZV0khuapYTR0RildcmJJLUQtDSkmYYxfo_fHumOLvGfKkg88W-t4MEOespSjZwlMLUByBizc5J2j1uDxn0k5TovdR6EMUeu-zpkofotCbhXd5EpibAO6ZdfJ-2b877jv_2G19At34aDsIuhRif0iJirB_4yiT3Q</recordid><startdate>19800925</startdate><enddate>19800925</enddate><creator>Karr, T L</creator><creator>Kristofferson, D</creator><creator>Purich, D L</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800925</creationdate><title>Mechanism of microtubule depolymerization. Correlation of rapid induced disassembly experiments with a kinetic model for endwise depolymerization</title><author>Karr, T L ; Kristofferson, D ; Purich, D L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-581b7dc2b29349baf6c41ddbf0a466ad6757486391d0584645a247595a02703d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Kinetics</topic><topic>Macromolecular Substances</topic><topic>Methods</topic><topic>Microtubules - ultrastructure</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karr, T L</creatorcontrib><creatorcontrib>Kristofferson, D</creatorcontrib><creatorcontrib>Purich, D L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karr, T L</au><au>Kristofferson, D</au><au>Purich, D L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of microtubule depolymerization. Correlation of rapid induced disassembly experiments with a kinetic model for endwise depolymerization</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1980-09-25</date><risdate>1980</risdate><volume>255</volume><issue>18</issue><spage>8560</spage><epage>8566</epage><pages>8560-8566</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Microtubule disassembly has been studied using a rapid dilution technique (Karr, T.L., and Purich, D.L. (1979) J. Biol. Chem.
254, 10885-10888). Disassembly curves, generated by computer from the solution of series first order differential equations
(see following paper), were fit to experimental data with excellent agreement when the diluted microtubules contained no microtubule-associated
proteins. The rate constant for dimer release from microtubules was found to be 154 s-1. Assuming a critical tubulin concentration
of 8 to 9 x 10(-6) M, the apparent bimolecular rate constant (2 x 10(7) M-1 s-1) for assembly is near the diffusion limit.
It was also possible to use the rapid dilution technique for quantitatively correlating the disassembly rate to the number
concentration of microtubule ends. These findings suggest that the dynamics of tubulin interactions with microtubules may
be characterized in terms of an endwise depolymerization model. A re-evaluation of cold induced depolymerization kinetics
(see miniprint supplement) is also fully consistent with our analysis of disassembly dynamics.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>7410377</pmid><doi>10.1016/S0021-9258(18)43534-X</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1980-09, Vol.255 (18), p.8560-8566 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Kinetics Macromolecular Substances Methods Microtubules - ultrastructure Tubulin - metabolism |
| title | Mechanism of microtubule depolymerization. Correlation of rapid induced disassembly experiments with a kinetic model for endwise depolymerization |
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