Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA
An enzymatic method is described for the analysis of the content of 5-methylcytosine in the DNA of eukaryotic cells. The conventional acid hydrolysis procedure was found to result in variable deamination of 5-methylcytosine into thymine, making the exact quantitation difficult. We applied the enzyma...
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| Veröffentlicht in: | The Journal of biological chemistry 1980-08, Vol.255 (16), p.7544-7547 |
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| container_title | The Journal of biological chemistry |
| container_volume | 255 |
| creator | d, J P Coca-Prados, M Hsu, M T |
| description | An enzymatic method is described for the analysis of the content of 5-methylcytosine in the DNA of eukaryotic cells. The conventional
acid hydrolysis procedure was found to result in variable deamination of 5-methylcytosine into thymine, making the exact quantitation
difficult. We applied the enzymatic method for the analysis of a simple eukaryotic viral genome. Viral and cell DNA in Simian
Virus 40-infected monkey kidney cells was labeled with [methyl-3H]methionine in vivo and their 5-methylcytosine content was
analyzed. The content of 5-methylcytosine per thymine in the total intracellular viral DNA was found to be less than 10(-4)
of the host DNA. The implication of this result with regard to eukaryotic gene expression is discussed. |
| doi_str_mv | 10.1016/S0021-9258(19)43861-1 |
| format | Article |
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acid hydrolysis procedure was found to result in variable deamination of 5-methylcytosine into thymine, making the exact quantitation
difficult. We applied the enzymatic method for the analysis of a simple eukaryotic viral genome. Viral and cell DNA in Simian
Virus 40-infected monkey kidney cells was labeled with [methyl-3H]methionine in vivo and their 5-methylcytosine content was
analyzed. The content of 5-methylcytosine per thymine in the total intracellular viral DNA was found to be less than 10(-4)
of the host DNA. The implication of this result with regard to eukaryotic gene expression is discussed.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)43861-1</identifier><identifier>PMID: 6249806</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>5-Methylcytosine ; Animals ; Cytosine - analogs & derivatives ; Cytosine - analysis ; Deoxyribonuclease I ; Deoxyribonucleases ; DNA, Viral - analysis ; Electrophoresis, Paper ; Endonucleases ; Haplorhini ; Methods ; Simian virus 40 - analysis</subject><ispartof>The Journal of biological chemistry, 1980-08, Vol.255 (16), p.7544-7547</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-2f3d7012ff09db922e3a57b94080e23deeb9183f46bbbf224002e6a39afecf5e3</citedby><cites>FETCH-LOGICAL-c379t-2f3d7012ff09db922e3a57b94080e23deeb9183f46bbbf224002e6a39afecf5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6249806$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>d, J P</creatorcontrib><creatorcontrib>Coca-Prados, M</creatorcontrib><creatorcontrib>Hsu, M T</creatorcontrib><title>Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>An enzymatic method is described for the analysis of the content of 5-methylcytosine in the DNA of eukaryotic cells. The conventional
acid hydrolysis procedure was found to result in variable deamination of 5-methylcytosine into thymine, making the exact quantitation
difficult. We applied the enzymatic method for the analysis of a simple eukaryotic viral genome. Viral and cell DNA in Simian
Virus 40-infected monkey kidney cells was labeled with [methyl-3H]methionine in vivo and their 5-methylcytosine content was
analyzed. The content of 5-methylcytosine per thymine in the total intracellular viral DNA was found to be less than 10(-4)
of the host DNA. The implication of this result with regard to eukaryotic gene expression is discussed.</description><subject>5-Methylcytosine</subject><subject>Animals</subject><subject>Cytosine - analogs & derivatives</subject><subject>Cytosine - analysis</subject><subject>Deoxyribonuclease I</subject><subject>Deoxyribonucleases</subject><subject>DNA, Viral - analysis</subject><subject>Electrophoresis, Paper</subject><subject>Endonucleases</subject><subject>Haplorhini</subject><subject>Methods</subject><subject>Simian virus 40 - analysis</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9P3DAQxa2qCLaUj7CSpUoVPQT8N4mPaLstSAgOSytulpOMu6aJQ21HKHz6Jrurncsc5r2ZeT-ElpRcUULz6w0hjGaKyfKSqm-ClznN6Ae0oKTkGZf0-SNaHCVn6FOML2QqoegpOs2ZUCXJF6hd-_exM8nV2HjTjtFF3Fsssw7SdmzrMfXRecB17xP4hJ3HMPw1Yexny_eHmyu8SUMzzibnUzA1tO3QmoA3rnPG498uDBELMms_oxNr2ggXh36Ofv1YP61us_vHn3erm_us5oVKGbO8KQhl1hLVVIox4EYWlRKkJMB4A1ApWnIr8qqqLGNiigm54cpYqK0Efo6-7ve-hv7fADHpzsX5MeOhH6IuJCO8oGoSyr2wDn2MAax-Da6b0mlK9ExZ7yjrGaGmSu8oazr5locDQ9VBc3QdsE7zL_v51v3ZvrkAunJ9vYVOMyn1tLaQQvD_kSWEYQ</recordid><startdate>19800825</startdate><enddate>19800825</enddate><creator>d, J P</creator><creator>Coca-Prados, M</creator><creator>Hsu, M T</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800825</creationdate><title>Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA</title><author>d, J P ; Coca-Prados, M ; Hsu, M T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-2f3d7012ff09db922e3a57b94080e23deeb9183f46bbbf224002e6a39afecf5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>5-Methylcytosine</topic><topic>Animals</topic><topic>Cytosine - analogs & derivatives</topic><topic>Cytosine - analysis</topic><topic>Deoxyribonuclease I</topic><topic>Deoxyribonucleases</topic><topic>DNA, Viral - analysis</topic><topic>Electrophoresis, Paper</topic><topic>Endonucleases</topic><topic>Haplorhini</topic><topic>Methods</topic><topic>Simian virus 40 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>d, J P</creatorcontrib><creatorcontrib>Coca-Prados, M</creatorcontrib><creatorcontrib>Hsu, M T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>d, J P</au><au>Coca-Prados, M</au><au>Hsu, M T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1980-08-25</date><risdate>1980</risdate><volume>255</volume><issue>16</issue><spage>7544</spage><epage>7547</epage><pages>7544-7547</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>An enzymatic method is described for the analysis of the content of 5-methylcytosine in the DNA of eukaryotic cells. The conventional
acid hydrolysis procedure was found to result in variable deamination of 5-methylcytosine into thymine, making the exact quantitation
difficult. We applied the enzymatic method for the analysis of a simple eukaryotic viral genome. Viral and cell DNA in Simian
Virus 40-infected monkey kidney cells was labeled with [methyl-3H]methionine in vivo and their 5-methylcytosine content was
analyzed. The content of 5-methylcytosine per thymine in the total intracellular viral DNA was found to be less than 10(-4)
of the host DNA. The implication of this result with regard to eukaryotic gene expression is discussed.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>6249806</pmid><doi>10.1016/S0021-9258(19)43861-1</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1980-08, Vol.255 (16), p.7544-7547 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | 5-Methylcytosine Animals Cytosine - analogs & derivatives Cytosine - analysis Deoxyribonuclease I Deoxyribonucleases DNA, Viral - analysis Electrophoresis, Paper Endonucleases Haplorhini Methods Simian virus 40 - analysis |
| title | Enzymatic analysis of 5-methylcytosine content in eukaryotic DNA. Study of intracellular Simian Virus 40 DNA |
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