Chicken reticulocyte nuclear antigen: its identification and relation to transcriptive activity in erythropoietic cells
Antibodies to chicken reticulocyte dehistonized chromatin were produced. These antibodies can distinguish between chromatin from reticulocytes and erythrocytes and have no activity with chicken liver chromatin. The antibodies will also differentiate between reticulocytes and erythrocytes by the the...
Gespeichert in:
| Veröffentlicht in: | Biochemistry (Easton) 1980-05, Vol.19 (11), p.2362-2367 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2367 |
|---|---|
| container_issue | 11 |
| container_start_page | 2362 |
| container_title | Biochemistry (Easton) |
| container_volume | 19 |
| creator | Pumo, Dorothy E Wierzbicki, Ryszard Chiu, Jen-Fu |
| description | Antibodies to chicken reticulocyte dehistonized chromatin were produced. These antibodies can distinguish between chromatin from reticulocytes and erythrocytes and have no activity with chicken liver chromatin. The antibodies will also differentiate between reticulocytes and erythrocytes by the the horseradish peroxidase bridge localization technique. The nonhistone protein components of the antigenic complex are very tightly bound to the DNA, removable by extraction with sodium dodecyl sulfate but not 2.5 M NaCl-5 M urea. Our data indicate that the antigen complexes could actually be present in both reticulocytes and erythrocytes but that chromatin condensation causes a structural masking of the complexes which coincides with the known decline in transcriptive activity of the erythrocyte. |
| doi_str_mv | 10.1021/bi00552a013 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75158985</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>75158985</sourcerecordid><originalsourceid>FETCH-LOGICAL-a354t-5372c3e1168516a1a7d0ef90f3fa4eff2db694bb1c316b51acb5c483b9f362da3</originalsourceid><addsrcrecordid>eNptkM1rFDEYxkNR2m3tybOQkx7KaDKZZCbe2vWjQkHFFUovIZN506adTbZJRt3_3iyzFA-eHh6e3_vBg9BLSt5SUtN3vSOE81oTyg7QgvKaVI2U_BlaEEJEVUtBjtBxSvfFNqRtDtGhkLLuuFyg38s7Zx7A4wjZmWkMZpsB-8mMoCPWPrtb8O-xywm7AYq1zujsgi_ZUIbG2eSAc9Q-meg22f0CrE0Rl7fYeQxxm-9i2AS3u4ENjGN6gZ5bPSY43esJ-vnp42p5WV19_fxleX5VacabXHHW1oYBpaLjVGiq24GAlcQyqxuwth56IZu-p4ZR0XOqTc9N07FeWibqQbMT9Hreu4nhcYKU1dql3QfaQ5iSajnlnex4Ac9m0MSQUgSrNtGtddwqStSuZvVPzYV-tV879WsYnth9ryWv5tylDH-eYh0flGhZy9Xq2w91wS5XN9fL7-pD4d_MvDZJ3Ycp-lLKfy__BYymlwA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75158985</pqid></control><display><type>article</type><title>Chicken reticulocyte nuclear antigen: its identification and relation to transcriptive activity in erythropoietic cells</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Pumo, Dorothy E ; Wierzbicki, Ryszard ; Chiu, Jen-Fu</creator><creatorcontrib>Pumo, Dorothy E ; Wierzbicki, Ryszard ; Chiu, Jen-Fu</creatorcontrib><description>Antibodies to chicken reticulocyte dehistonized chromatin were produced. These antibodies can distinguish between chromatin from reticulocytes and erythrocytes and have no activity with chicken liver chromatin. The antibodies will also differentiate between reticulocytes and erythrocytes by the the horseradish peroxidase bridge localization technique. The nonhistone protein components of the antigenic complex are very tightly bound to the DNA, removable by extraction with sodium dodecyl sulfate but not 2.5 M NaCl-5 M urea. Our data indicate that the antigen complexes could actually be present in both reticulocytes and erythrocytes but that chromatin condensation causes a structural masking of the complexes which coincides with the known decline in transcriptive activity of the erythrocyte.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00552a013</identifier><identifier>PMID: 6992859</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Antigens - analysis ; Cell Nucleus - immunology ; Cell Nucleus - metabolism ; Chickens ; Chromatin - immunology ; Chromatin - metabolism ; Chromosomal Proteins, Non-Histone - blood ; Chromosomal Proteins, Non-Histone - immunology ; Complement Fixation Tests ; Erythrocytes - immunology ; Erythrocytes - metabolism ; Horseradish Peroxidase ; Immunoenzyme Techniques ; Male ; Reticulocytes - metabolism ; Transcription, Genetic</subject><ispartof>Biochemistry (Easton), 1980-05, Vol.19 (11), p.2362-2367</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a354t-5372c3e1168516a1a7d0ef90f3fa4eff2db694bb1c316b51acb5c483b9f362da3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00552a013$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00552a013$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6992859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pumo, Dorothy E</creatorcontrib><creatorcontrib>Wierzbicki, Ryszard</creatorcontrib><creatorcontrib>Chiu, Jen-Fu</creatorcontrib><title>Chicken reticulocyte nuclear antigen: its identification and relation to transcriptive activity in erythropoietic cells</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>Antibodies to chicken reticulocyte dehistonized chromatin were produced. These antibodies can distinguish between chromatin from reticulocytes and erythrocytes and have no activity with chicken liver chromatin. The antibodies will also differentiate between reticulocytes and erythrocytes by the the horseradish peroxidase bridge localization technique. The nonhistone protein components of the antigenic complex are very tightly bound to the DNA, removable by extraction with sodium dodecyl sulfate but not 2.5 M NaCl-5 M urea. Our data indicate that the antigen complexes could actually be present in both reticulocytes and erythrocytes but that chromatin condensation causes a structural masking of the complexes which coincides with the known decline in transcriptive activity of the erythrocyte.</description><subject>Animals</subject><subject>Antigens - analysis</subject><subject>Cell Nucleus - immunology</subject><subject>Cell Nucleus - metabolism</subject><subject>Chickens</subject><subject>Chromatin - immunology</subject><subject>Chromatin - metabolism</subject><subject>Chromosomal Proteins, Non-Histone - blood</subject><subject>Chromosomal Proteins, Non-Histone - immunology</subject><subject>Complement Fixation Tests</subject><subject>Erythrocytes - immunology</subject><subject>Erythrocytes - metabolism</subject><subject>Horseradish Peroxidase</subject><subject>Immunoenzyme Techniques</subject><subject>Male</subject><subject>Reticulocytes - metabolism</subject><subject>Transcription, Genetic</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM1rFDEYxkNR2m3tybOQkx7KaDKZZCbe2vWjQkHFFUovIZN506adTbZJRt3_3iyzFA-eHh6e3_vBg9BLSt5SUtN3vSOE81oTyg7QgvKaVI2U_BlaEEJEVUtBjtBxSvfFNqRtDtGhkLLuuFyg38s7Zx7A4wjZmWkMZpsB-8mMoCPWPrtb8O-xywm7AYq1zujsgi_ZUIbG2eSAc9Q-meg22f0CrE0Rl7fYeQxxm-9i2AS3u4ENjGN6gZ5bPSY43esJ-vnp42p5WV19_fxleX5VacabXHHW1oYBpaLjVGiq24GAlcQyqxuwth56IZu-p4ZR0XOqTc9N07FeWibqQbMT9Hreu4nhcYKU1dql3QfaQ5iSajnlnex4Ac9m0MSQUgSrNtGtddwqStSuZvVPzYV-tV879WsYnth9ryWv5tylDH-eYh0flGhZy9Xq2w91wS5XN9fL7-pD4d_MvDZJ3Ycp-lLKfy__BYymlwA</recordid><startdate>19800527</startdate><enddate>19800527</enddate><creator>Pumo, Dorothy E</creator><creator>Wierzbicki, Ryszard</creator><creator>Chiu, Jen-Fu</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800527</creationdate><title>Chicken reticulocyte nuclear antigen: its identification and relation to transcriptive activity in erythropoietic cells</title><author>Pumo, Dorothy E ; Wierzbicki, Ryszard ; Chiu, Jen-Fu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a354t-5372c3e1168516a1a7d0ef90f3fa4eff2db694bb1c316b51acb5c483b9f362da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Antigens - analysis</topic><topic>Cell Nucleus - immunology</topic><topic>Cell Nucleus - metabolism</topic><topic>Chickens</topic><topic>Chromatin - immunology</topic><topic>Chromatin - metabolism</topic><topic>Chromosomal Proteins, Non-Histone - blood</topic><topic>Chromosomal Proteins, Non-Histone - immunology</topic><topic>Complement Fixation Tests</topic><topic>Erythrocytes - immunology</topic><topic>Erythrocytes - metabolism</topic><topic>Horseradish Peroxidase</topic><topic>Immunoenzyme Techniques</topic><topic>Male</topic><topic>Reticulocytes - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pumo, Dorothy E</creatorcontrib><creatorcontrib>Wierzbicki, Ryszard</creatorcontrib><creatorcontrib>Chiu, Jen-Fu</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pumo, Dorothy E</au><au>Wierzbicki, Ryszard</au><au>Chiu, Jen-Fu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chicken reticulocyte nuclear antigen: its identification and relation to transcriptive activity in erythropoietic cells</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1980-05-27</date><risdate>1980</risdate><volume>19</volume><issue>11</issue><spage>2362</spage><epage>2367</epage><pages>2362-2367</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Antibodies to chicken reticulocyte dehistonized chromatin were produced. These antibodies can distinguish between chromatin from reticulocytes and erythrocytes and have no activity with chicken liver chromatin. The antibodies will also differentiate between reticulocytes and erythrocytes by the the horseradish peroxidase bridge localization technique. The nonhistone protein components of the antigenic complex are very tightly bound to the DNA, removable by extraction with sodium dodecyl sulfate but not 2.5 M NaCl-5 M urea. Our data indicate that the antigen complexes could actually be present in both reticulocytes and erythrocytes but that chromatin condensation causes a structural masking of the complexes which coincides with the known decline in transcriptive activity of the erythrocyte.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>6992859</pmid><doi>10.1021/bi00552a013</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2960 |
| ispartof | Biochemistry (Easton), 1980-05, Vol.19 (11), p.2362-2367 |
| issn | 0006-2960 1520-4995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75158985 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Antigens - analysis Cell Nucleus - immunology Cell Nucleus - metabolism Chickens Chromatin - immunology Chromatin - metabolism Chromosomal Proteins, Non-Histone - blood Chromosomal Proteins, Non-Histone - immunology Complement Fixation Tests Erythrocytes - immunology Erythrocytes - metabolism Horseradish Peroxidase Immunoenzyme Techniques Male Reticulocytes - metabolism Transcription, Genetic |
| title | Chicken reticulocyte nuclear antigen: its identification and relation to transcriptive activity in erythropoietic cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A10%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chicken%20reticulocyte%20nuclear%20antigen:%20its%20identification%20and%20relation%20to%20transcriptive%20activity%20in%20erythropoietic%20cells&rft.jtitle=Biochemistry%20(Easton)&rft.au=Pumo,%20Dorothy%20E&rft.date=1980-05-27&rft.volume=19&rft.issue=11&rft.spage=2362&rft.epage=2367&rft.pages=2362-2367&rft.issn=0006-2960&rft.eissn=1520-4995&rft_id=info:doi/10.1021/bi00552a013&rft_dat=%3Cproquest_cross%3E75158985%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75158985&rft_id=info:pmid/6992859&rfr_iscdi=true |