[6] Affinity chromatography of NAD on immobilized dehydrogenase columns

This chapter discusses the results of affinity chromatography of nicotinamide adenine dinucleotide (NAD) on immobilized dehydrogenase columns. For immobilization of alcohol dehydrogenase to glutaraldehydetreated AE-cellulose, 150 ml of 0.5 M NaOH are added to 10 g (dry weight) of support material. A...

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Veröffentlicht in:	Methods in Enzymology 1980, Vol.66, p.39-50
1. Verfasser:	Das, Kumudeswar
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - isolation & purification Adenosine Monophosphate - isolation & purification Adenosine Triphosphate - isolation & purification Alcohol Oxidoreductases - metabolism Chromatography, Affinity - methods Enzymes, Immobilized - metabolism L-Lactate Dehydrogenase - metabolism NAD - isolation & purification Saccharomyces cerevisiae - analysis
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