[10] Subsite mapping of enzymes: Application to polysaccharide depolymerases

This chapter discusses subsite mapping, the term applied to the experimental determination of the number of subsites, comprising the binding region, the energetics of interaction of each subsite with a monomer residue, and the hydrolytic rate coefficients. The value of the generated subsite map lies...

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Veröffentlicht in:	Methods in Enzymology 1980, Vol.64, p.248-277
1. Verfasser:	Allen, Jimmy D.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - metabolism Bacillus - enzymology Binding Sites Glycoside Hydrolases - metabolism Kinetics Mathematics Methods Oligosaccharides Polysaccharides Protein Binding Substrate Specificity Thermodynamics
Online-Zugang:	Volltext
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