Control of glycoprotein synthesis. The purification by preparative high voltage paper electrophoresis in borate of glycopeptides containing high mannose and complex oligosaccharide chains linked to asparagine

The heterogeneity of glycopeptides prepared from pronase digests of various glycoproteins has previously been demonstrated primarily by lengthy paper chromatography of oligosaccharides released from the glycopeptides by enzymatic or chemical means. We describe the use of high voltage paper electroph...

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Veröffentlicht in:	The Journal of biological chemistry 1980-05, Vol.255 (10), p.4876-4884
Hauptverfasser:	Narasimhan, S, Harpaz, N, Longmore, G, Carver, J P, Grey, A A, Schachter, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Asparagine - analysis Carbohydrate Conformation Carbohydrate Sequence Glycopeptides - isolation & purification Glycoproteins - biosynthesis Humans Immunoglobulin G Mannose - analysis Oligosaccharides - isolation & purification Ovalbumin Protein Binding
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