Specific control of messenger translation in Drosophila oocytes and embryos
The distribution of messenger RNA between polysomes and mRNP in oocytes and embryos of Drosophila melanogaster has been studied by in vitro translational analysis. Poly(A) + RNA was purified from polysomes or mRNA from mature oocytes and young embryos. The messenger populations were translated in vi...
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| Veröffentlicht in: | Developmental biology 1980-03, Vol.75 (1), p.177-186 |
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| creator | Mermod, J.J. Schatz, G. Crippa, M. |
| description | The distribution of messenger RNA between polysomes and mRNP in oocytes and embryos of
Drosophila melanogaster has been studied by
in vitro translational analysis. Poly(A)
+ RNA was purified from polysomes or mRNA from mature oocytes and young embryos. The messenger populations were translated
in vitro and the peptides synthesized were separated by two-dimensional electrophoresis. Analysis of the 2D gel patterns enabled the detection of three peptides coded by messengers present predominantly in the mRNA pools of mature oocytes. When DNA-binding peptides were selected from the
in vitro translation products, they showed, after separation by two-dimensional electrophoresis, less than 100 spots. The analysis of the 2D gels indicated that three DNA-binding peptides are coded by messengers present only in the mRNP of the oocytes. These messengers are later found in the polysomal fraction of embryos. |
| doi_str_mv | 10.1016/0012-1606(80)90153-0 |
| format | Article |
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Drosophila melanogaster has been studied by
in vitro translational analysis. Poly(A)
+ RNA was purified from polysomes or mRNA from mature oocytes and young embryos. The messenger populations were translated
in vitro and the peptides synthesized were separated by two-dimensional electrophoresis. Analysis of the 2D gel patterns enabled the detection of three peptides coded by messengers present predominantly in the mRNA pools of mature oocytes. When DNA-binding peptides were selected from the
in vitro translation products, they showed, after separation by two-dimensional electrophoresis, less than 100 spots. The analysis of the 2D gels indicated that three DNA-binding peptides are coded by messengers present only in the mRNP of the oocytes. These messengers are later found in the polysomal fraction of embryos.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/0012-1606(80)90153-0</identifier><identifier>PMID: 6768627</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chromatography, Agarose ; Drosophila melanogaster - genetics ; Electrophoresis, Polyacrylamide Gel ; Embryo, Nonmammalian - metabolism ; Female ; Isoelectric Focusing ; Oocytes - metabolism ; Poly A - metabolism ; Protein Biosynthesis ; Ribosomes - metabolism ; RNA, Messenger - genetics</subject><ispartof>Developmental biology, 1980-03, Vol.75 (1), p.177-186</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-bdc133a915b07c7675db6e36a30fe4a0fef3a501a538e6f3bac75c079942ec703</citedby><cites>FETCH-LOGICAL-c380t-bdc133a915b07c7675db6e36a30fe4a0fef3a501a538e6f3bac75c079942ec703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0012160680901530$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6768627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mermod, J.J.</creatorcontrib><creatorcontrib>Schatz, G.</creatorcontrib><creatorcontrib>Crippa, M.</creatorcontrib><creatorcontrib>North Texas State University (USA)</creatorcontrib><title>Specific control of messenger translation in Drosophila oocytes and embryos</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>The distribution of messenger RNA between polysomes and mRNP in oocytes and embryos of
Drosophila melanogaster has been studied by
in vitro translational analysis. Poly(A)
+ RNA was purified from polysomes or mRNA from mature oocytes and young embryos. The messenger populations were translated
in vitro and the peptides synthesized were separated by two-dimensional electrophoresis. Analysis of the 2D gel patterns enabled the detection of three peptides coded by messengers present predominantly in the mRNA pools of mature oocytes. When DNA-binding peptides were selected from the
in vitro translation products, they showed, after separation by two-dimensional electrophoresis, less than 100 spots. The analysis of the 2D gels indicated that three DNA-binding peptides are coded by messengers present only in the mRNP of the oocytes. These messengers are later found in the polysomal fraction of embryos.</description><subject>Animals</subject><subject>Chromatography, Agarose</subject><subject>Drosophila melanogaster - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Embryo, Nonmammalian - metabolism</subject><subject>Female</subject><subject>Isoelectric Focusing</subject><subject>Oocytes - metabolism</subject><subject>Poly A - metabolism</subject><subject>Protein Biosynthesis</subject><subject>Ribosomes - metabolism</subject><subject>RNA, Messenger - genetics</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKBDEQRYMoOj7-QCUr0UVrZTJ5bQTxjYILFdyFdLpaI92dMekR5u_tcQaXbqoW99SlOITsMzhlwOQZABsXTII81nBigAlewBoZMTCiEHLytk5Gf8gW2c75EwC41nyTbEoltRyrEXl4nqIPdfDUx65PsaGxpi3mjN07Jton1-XG9SF2NHT0KsUcpx-hcTRGP-8xU9dVFNsyzWPeJRu1azLurfYOeb25frm8Kx6fbu8vLx4LzzX0RVl5xrkzTJSgvJJKVKVELh2HGiduGDV3ApgTXKOseem8Eh6UMZMxegV8hxwte6cpfs0w97YN2WPTuA7jLFslwGgDfAAnS9APf-eEtZ2m0Lo0twzswqFdCLILQVaD_XVoF_0Hq_5Z2WL1d7SSNuSHy7x20br3FLJ9fWZGM5BcGSkH4HwJ4GDhO2Cy2QfsPFYhoe9tFcP_H_wAPJqJ-w</recordid><startdate>19800301</startdate><enddate>19800301</enddate><creator>Mermod, J.J.</creator><creator>Schatz, G.</creator><creator>Crippa, M.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800301</creationdate><title>Specific control of messenger translation in Drosophila oocytes and embryos</title><author>Mermod, J.J. ; Schatz, G. ; Crippa, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-bdc133a915b07c7675db6e36a30fe4a0fef3a501a538e6f3bac75c079942ec703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Chromatography, Agarose</topic><topic>Drosophila melanogaster - genetics</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Embryo, Nonmammalian - metabolism</topic><topic>Female</topic><topic>Isoelectric Focusing</topic><topic>Oocytes - metabolism</topic><topic>Poly A - metabolism</topic><topic>Protein Biosynthesis</topic><topic>Ribosomes - metabolism</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mermod, J.J.</creatorcontrib><creatorcontrib>Schatz, G.</creatorcontrib><creatorcontrib>Crippa, M.</creatorcontrib><creatorcontrib>North Texas State University (USA)</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mermod, J.J.</au><au>Schatz, G.</au><au>Crippa, M.</au><aucorp>North Texas State University (USA)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific control of messenger translation in Drosophila oocytes and embryos</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1980-03-01</date><risdate>1980</risdate><volume>75</volume><issue>1</issue><spage>177</spage><epage>186</epage><pages>177-186</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>The distribution of messenger RNA between polysomes and mRNP in oocytes and embryos of
Drosophila melanogaster has been studied by
in vitro translational analysis. Poly(A)
+ RNA was purified from polysomes or mRNA from mature oocytes and young embryos. The messenger populations were translated
in vitro and the peptides synthesized were separated by two-dimensional electrophoresis. Analysis of the 2D gel patterns enabled the detection of three peptides coded by messengers present predominantly in the mRNA pools of mature oocytes. When DNA-binding peptides were selected from the
in vitro translation products, they showed, after separation by two-dimensional electrophoresis, less than 100 spots. The analysis of the 2D gels indicated that three DNA-binding peptides are coded by messengers present only in the mRNP of the oocytes. These messengers are later found in the polysomal fraction of embryos.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6768627</pmid><doi>10.1016/0012-1606(80)90153-0</doi><tpages>10</tpages></addata></record> |
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| ispartof | Developmental biology, 1980-03, Vol.75 (1), p.177-186 |
| issn | 0012-1606 1095-564X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Chromatography, Agarose Drosophila melanogaster - genetics Electrophoresis, Polyacrylamide Gel Embryo, Nonmammalian - metabolism Female Isoelectric Focusing Oocytes - metabolism Poly A - metabolism Protein Biosynthesis Ribosomes - metabolism RNA, Messenger - genetics |
| title | Specific control of messenger translation in Drosophila oocytes and embryos |
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