Initiation factor eIF-2 from wheat germ. Purification functional comparison to eIF-2 from rabbit reticulocytes and phosphorylation of its subunits

Protein synthesis initiation factor eIF‐2 was isolated and purified 4300‐fold from wheat germ 23000 × g supernatant. The procedure described resulted in a 70% pure factor and involves three chromatographic steps only: (a) on Sepharose‐heparin, (b) on Sepharose–concanavalin‐A and (c) on phosphocellul...

Ausführliche Beschreibung