Immunomicrospheres: Reagents for Cell Labeling and Separation
Immunomicrospheres are specially designed microscopic particles that have antibodies or similar molecules chemically bound to their surfaces. The antibody-coated microspheres react in a highly specific way with target cells, viruses, or other antigenic agents. Immunomicrospheres may be synthesized s...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1980-04, Vol.208 (4442), p.364-368 |
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| creator | Rembaum, A. Dreyer, W. J. |
| description | Immunomicrospheres are specially designed microscopic particles that have antibodies or similar molecules chemically bound to their surfaces. The antibody-coated microspheres react in a highly specific way with target cells, viruses, or other antigenic agents. Immunomicrospheres may be synthesized so that they incorporate compounds that are highly radioactive, intensely fluorescent, magnetic, electron opaque, highly colored, or pharmacologically active. These various types of microspheres may be coated with pure, highly specific monoclonal antibodies obtained by the new hybridoma cell cloning techniques or with conventional antibody preparations. Some of the many present and potential applications for these new reagents are (i) new types of radioimmune or immunofluorescent assays, (ii) improved fluorescence microscopy, (iii) separation of cells on the basis of the fluorescent, electrophoretic, or magnetic properties of bound immunomicrospheres, (iv) markers for use in several types of electron or standard light microscopy, and (v) delivery of lethal compounds to specific undesirable living cells. The combination of the various new types of synthetic microspheres and the newly available homogeneous antibodies offers new opportunities in research, diagnosis, and therapy. |
| doi_str_mv | 10.1126/science.6768131 |
| format | Article |
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J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunomicrospheres: Reagents for Cell Labeling and Separation</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1980-04-25</date><risdate>1980</risdate><volume>208</volume><issue>4442</issue><spage>364</spage><epage>368</epage><pages>364-368</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Immunomicrospheres are specially designed microscopic particles that have antibodies or similar molecules chemically bound to their surfaces. The antibody-coated microspheres react in a highly specific way with target cells, viruses, or other antigenic agents. Immunomicrospheres may be synthesized so that they incorporate compounds that are highly radioactive, intensely fluorescent, magnetic, electron opaque, highly colored, or pharmacologically active. 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The combination of the various new types of synthetic microspheres and the newly available homogeneous antibodies offers new opportunities in research, diagnosis, and therapy.</abstract><cop>United States</cop><pub>The American Association for the Advancement of Science</pub><pmid>6768131</pmid><doi>10.1126/science.6768131</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1980-04, Vol.208 (4442), p.364-368 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75075795 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Active sites (Biochemistry) Analysis Animals Antibodies Antigens Antigens, Neoplasm - analysis B lymphocytes Binding sites (Biochemistry) Cell Separation - methods Electrophoresis Erythrocytes Fluorescent Antibody Technique Glutaral - analogs & derivatives Humans Immunologic research Immunological research Immunosorbent Techniques Immunospecificity Lymphocytes Magnetics Membrane Proteins - analysis Microscopy, Electron - methods Microscopy, Electron, Scanning Microspheres Molecules Physiological aspects Polymerization Polymethacrylic Acids Reagents T lymphocytes Tumors |
| title | Immunomicrospheres: Reagents for Cell Labeling and Separation |
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