Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic cell line, HL-60
The human promyelocytic leukemia cell line HL-60 can be grown in serum-free synthetic medium supplemented with insulin and transferrin alone. Growth of HL-60 in this defined medium is at a rate approx. 80% of that in medium containing serum. Moreover, the distinct morphological and histochemical mye...
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| Veröffentlicht in: | Experimental cell research 1980-04, Vol.126 (2), p.494-498 |
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| container_title | Experimental cell research |
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| creator | Breitman, T.R. Collins, S.J. Keene, B.R. |
| description | The human promyelocytic leukemia cell line HL-60 can be grown in serum-free synthetic medium supplemented with insulin and transferrin alone. Growth of HL-60 in this defined medium is at a rate approx. 80% of that in medium containing serum. Moreover, the distinct morphological and histochemical myeloid characteristics of HL-60 are maintained in such serum-free medium. The HL-60 promyelocytes are induced by DMSO to differentiate to mature granulocytes equally well in both serum-supplemented and serum-free medium. However, this defined medium does not support colony growth of HL-60 in semi-solid medium such as methylcellulose. |
| doi_str_mv | 10.1016/0014-4827(80)90296-7 |
| format | Article |
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| ispartof | Experimental cell research, 1980-04, Vol.126 (2), p.494-498 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Cell Differentiation - drug effects Cell Division - drug effects Cell Line Clone Cells Culture Media Humans Insulin - pharmacology Leukemia, Myeloid Selenium - pharmacology Transferrin - pharmacology |
| title | Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic cell line, HL-60 |
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