Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria
Based on the effects of ionomycin upon mitochondrial respiration, ionomycin was shown to be an effective ionophore for Ca2+ in rat liver mitochondria. The ionomycin-induced efflux of Ca2+ across the inner membrane was more sensitive to loading the mitochondria with Ca2+ than was efflux catalyzed by...
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| Veröffentlicht in: | The Journal of biological chemistry 1980-04, Vol.255 (7), p.2735-2739 |
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| container_title | The Journal of biological chemistry |
| container_volume | 255 |
| creator | Kauffman, R F Taylor, R W Pfeiffer, D R |
| description | Based on the effects of ionomycin upon mitochondrial respiration, ionomycin was shown to be an effective ionophore for Ca2+
in rat liver mitochondria. The ionomycin-induced efflux of Ca2+ across the inner membrane was more sensitive to loading the
mitochondria with Ca2+ than was efflux catalyzed by A23187. At saturating concentrations of Ca2+, the turnover number for
ionomycin was 3- to 5-fold greater than that of A23187. Ionomycin catalyzed the efflux of mitochondrial Mg2+ at rates comparable
to those observed with A23187. Ionomycin also mediated an efflux of K+ provided that the mitochondria were depleted of their
endogenous divalent metal ions. The apparent turnover numbers for K+ efflux suggest that ionomycin is more specific for divalent
metal ions than A23187. |
| doi_str_mv | 10.1016/S0021-9258(19)85799-X |
| format | Article |
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in rat liver mitochondria. The ionomycin-induced efflux of Ca2+ across the inner membrane was more sensitive to loading the
mitochondria with Ca2+ than was efflux catalyzed by A23187. At saturating concentrations of Ca2+, the turnover number for
ionomycin was 3- to 5-fold greater than that of A23187. Ionomycin catalyzed the efflux of mitochondrial Mg2+ at rates comparable
to those observed with A23187. Ionomycin also mediated an efflux of K+ provided that the mitochondria were depleted of their
endogenous divalent metal ions. The apparent turnover numbers for K+ efflux suggest that ionomycin is more specific for divalent
metal ions than A23187.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)85799-X</identifier><identifier>PMID: 6766939</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Biological Transport, Active - drug effects ; Calcimycin - pharmacology ; Calcium - metabolism ; Ethers - pharmacology ; Hydrogen-Ion Concentration ; Ionomycin ; Kinetics ; Magnesium - metabolism ; Mitochondria, Liver - drug effects ; Mitochondria, Liver - metabolism ; Oxygen Consumption - drug effects ; Potassium - metabolism ; Rats ; Spectrophotometry, Ultraviolet ; Succinates - metabolism</subject><ispartof>The Journal of biological chemistry, 1980-04, Vol.255 (7), p.2735-2739</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-289776788c80982996179561bbf3db9abc81725495b8bee3337aa188d1789da33</citedby><cites>FETCH-LOGICAL-c378t-289776788c80982996179561bbf3db9abc81725495b8bee3337aa188d1789da33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6766939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kauffman, R F</creatorcontrib><creatorcontrib>Taylor, R W</creatorcontrib><creatorcontrib>Pfeiffer, D R</creatorcontrib><title>Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Based on the effects of ionomycin upon mitochondrial respiration, ionomycin was shown to be an effective ionophore for Ca2+
in rat liver mitochondria. The ionomycin-induced efflux of Ca2+ across the inner membrane was more sensitive to loading the
mitochondria with Ca2+ than was efflux catalyzed by A23187. At saturating concentrations of Ca2+, the turnover number for
ionomycin was 3- to 5-fold greater than that of A23187. Ionomycin catalyzed the efflux of mitochondrial Mg2+ at rates comparable
to those observed with A23187. Ionomycin also mediated an efflux of K+ provided that the mitochondria were depleted of their
endogenous divalent metal ions. The apparent turnover numbers for K+ efflux suggest that ionomycin is more specific for divalent
metal ions than A23187.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biological Transport, Active - drug effects</subject><subject>Calcimycin - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Ethers - pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Ionomycin</subject><subject>Kinetics</subject><subject>Magnesium - metabolism</subject><subject>Mitochondria, Liver - drug effects</subject><subject>Mitochondria, Liver - metabolism</subject><subject>Oxygen Consumption - drug effects</subject><subject>Potassium - metabolism</subject><subject>Rats</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Succinates - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtKxDAUhoMoOo4-ghBciC6quZgmWcrgDQQXKswupGlqj0ybmmSUeXs7Fzybs_i__xz4EDqj5JoSWt68EcJooZlQl1RfKSG1LuZ7aEKJ4gUXdL6PJv_IETpO6YuMc6vpITosZVlqricIZjZD6HGOtk9DiBnbvsZp8A4acJBXODR4BEK3ctBf41noBhshjZVfyO0mGtoQPb5jnCqJocfRZryAHx9xBzm4NvR1BHuCDhq7SP50t6fo4-H-ffZUvLw-Ps_uXgrHpcoFU1rKUirlFNGKaV1SqUVJq6rhdaVt5RSVTNxqUanKe865tJYqVVOpdG05n6KL7d0hhu-lT9l0kJxfLGzvwzIZKUjJhGQjKLagiyGl6BszROhsXBlKzFqx2Sg2a3-GarNRbOZj72z3YFl1vv5v7ZyO-fk2b-Gz_YXoTQWjBd8ZJoSRhkku-B9B74LN</recordid><startdate>19800410</startdate><enddate>19800410</enddate><creator>Kauffman, R F</creator><creator>Taylor, R W</creator><creator>Pfeiffer, D R</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800410</creationdate><title>Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria</title><author>Kauffman, R F ; Taylor, R W ; Pfeiffer, D R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-289776788c80982996179561bbf3db9abc81725495b8bee3337aa188d1789da33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biological Transport, Active - drug effects</topic><topic>Calcimycin - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Ethers - pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Ionomycin</topic><topic>Kinetics</topic><topic>Magnesium - metabolism</topic><topic>Mitochondria, Liver - drug effects</topic><topic>Mitochondria, Liver - metabolism</topic><topic>Oxygen Consumption - drug effects</topic><topic>Potassium - metabolism</topic><topic>Rats</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Succinates - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kauffman, R F</creatorcontrib><creatorcontrib>Taylor, R W</creatorcontrib><creatorcontrib>Pfeiffer, D R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kauffman, R F</au><au>Taylor, R W</au><au>Pfeiffer, D R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1980-04-10</date><risdate>1980</risdate><volume>255</volume><issue>7</issue><spage>2735</spage><epage>2739</epage><pages>2735-2739</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Based on the effects of ionomycin upon mitochondrial respiration, ionomycin was shown to be an effective ionophore for Ca2+
in rat liver mitochondria. The ionomycin-induced efflux of Ca2+ across the inner membrane was more sensitive to loading the
mitochondria with Ca2+ than was efflux catalyzed by A23187. At saturating concentrations of Ca2+, the turnover number for
ionomycin was 3- to 5-fold greater than that of A23187. Ionomycin catalyzed the efflux of mitochondrial Mg2+ at rates comparable
to those observed with A23187. Ionomycin also mediated an efflux of K+ provided that the mitochondria were depleted of their
endogenous divalent metal ions. The apparent turnover numbers for K+ efflux suggest that ionomycin is more specific for divalent
metal ions than A23187.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>6766939</pmid><doi>10.1016/S0021-9258(19)85799-X</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1980-04, Vol.255 (7), p.2735-2739 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75062572 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Anti-Bacterial Agents - pharmacology Biological Transport, Active - drug effects Calcimycin - pharmacology Calcium - metabolism Ethers - pharmacology Hydrogen-Ion Concentration Ionomycin Kinetics Magnesium - metabolism Mitochondria, Liver - drug effects Mitochondria, Liver - metabolism Oxygen Consumption - drug effects Potassium - metabolism Rats Spectrophotometry, Ultraviolet Succinates - metabolism |
| title | Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria |
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