The status of glycolaldehyde in the biosynthesis of vitamin B6
Competition experiments, employing 14C-labeled samples of glycerol and glycolaldehyde, indicate that in Escherichia coli B there are two independent pathways leading to pyridoxal. In mutant WG2 (and therefore presumably also in the wild strain) the major pathway utilizes glycerol and related trioses...
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| Veröffentlicht in: | The Journal of biological chemistry 1980-04, Vol.255 (7), p.3042-3048 |
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| container_title | The Journal of biological chemistry |
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| creator | Vella, G J Hill, R E Mootoo, B S Spenser, I D |
| description | Competition experiments, employing 14C-labeled samples of glycerol and glycolaldehyde, indicate that in Escherichia coli B
there are two independent pathways leading to pyridoxal. In mutant WG2 (and therefore presumably also in the wild strain)
the major pathway utilizes glycerol and related trioses as the sole carbon source in the construction of the C8N skeleton
of pyridoxol: C-1, -3 of glycerol yields C-2', -3, -4', -5' and -6, C-2 of glycerol yields C-2, -4, and -5 of the vitamin.
In the minor pathway glycolaldehyde and not glycerol supplies C-5 and C-5' of pyridoxol, while glycerol is the source of the
other 6 carbon atoms. In mutant WG3 the major route is blocked and the "glycolaldehyde pathway" becomes the sole source of
vitamin B6. |
| doi_str_mv | 10.1016/S0021-9258(19)85849-0 |
| format | Article |
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there are two independent pathways leading to pyridoxal. In mutant WG2 (and therefore presumably also in the wild strain)
the major pathway utilizes glycerol and related trioses as the sole carbon source in the construction of the C8N skeleton
of pyridoxol: C-1, -3 of glycerol yields C-2', -3, -4', -5' and -6, C-2 of glycerol yields C-2, -4, and -5 of the vitamin.
In the minor pathway glycolaldehyde and not glycerol supplies C-5 and C-5' of pyridoxol, while glycerol is the source of the
other 6 carbon atoms. In mutant WG3 the major route is blocked and the "glycolaldehyde pathway" becomes the sole source of
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there are two independent pathways leading to pyridoxal. In mutant WG2 (and therefore presumably also in the wild strain)
the major pathway utilizes glycerol and related trioses as the sole carbon source in the construction of the C8N skeleton
of pyridoxol: C-1, -3 of glycerol yields C-2', -3, -4', -5' and -6, C-2 of glycerol yields C-2, -4, and -5 of the vitamin.
In the minor pathway glycolaldehyde and not glycerol supplies C-5 and C-5' of pyridoxol, while glycerol is the source of the
other 6 carbon atoms. In mutant WG3 the major route is blocked and the "glycolaldehyde pathway" becomes the sole source of
vitamin B6.</description><subject>Acetaldehyde - analogs & derivatives</subject><subject>Acetaldehyde - metabolism</subject><subject>Carbon Radioisotopes</subject><subject>Escherichia coli - metabolism</subject><subject>Glycerol - metabolism</subject><subject>Isotope Labeling</subject><subject>Mutation</subject><subject>Pyridoxine - biosynthesis</subject><subject>Species Specificity</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMo67r6ExaKB9FDNWmadnIRdPELFjy4greQppNtpd2uTav035v9YOcyA887M_AQMmX0llGW3H1QGrFQRgKumbwBAbEM6REZMwo85IJ9HZPxIXJKzpz7pr5iyUZklEhII87G5H5RYOA63fUuaGywrAbTVLrKsRhyDMpV0HmelY0bVn5y5Tb1W3a69uwxOScnVlcOL_Z9Qj6fnxaz13D-_vI2e5iHhqfQhUxnVvKcxxowZQatjjlmNgPK0iiXMRqubWw5A8gsBUxyTEAaIYClBsDyCbna3V23zU-PrlN16QxWlV5h0zuVCspBUOmDYhc0beNci1at27LW7aAYVRtvautNbaQoJtXWm6J-b7p_0Gc15oetvSjPL3e8KJfFX9mi8lJMgbWKhFCp4jSO-D_hoHQ5</recordid><startdate>19800410</startdate><enddate>19800410</enddate><creator>Vella, G J</creator><creator>Hill, R E</creator><creator>Mootoo, B S</creator><creator>Spenser, I D</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800410</creationdate><title>The status of glycolaldehyde in the biosynthesis of vitamin B6</title><author>Vella, G J ; Hill, R E ; Mootoo, B S ; Spenser, I D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-1abf93d34a8e71cefa43ebfb80172d94ec3af4f3188bf08e6de689c55817c88f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Acetaldehyde - analogs & derivatives</topic><topic>Acetaldehyde - metabolism</topic><topic>Carbon Radioisotopes</topic><topic>Escherichia coli - metabolism</topic><topic>Glycerol - metabolism</topic><topic>Isotope Labeling</topic><topic>Mutation</topic><topic>Pyridoxine - biosynthesis</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vella, G J</creatorcontrib><creatorcontrib>Hill, R E</creatorcontrib><creatorcontrib>Mootoo, B S</creatorcontrib><creatorcontrib>Spenser, I D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vella, G J</au><au>Hill, R E</au><au>Mootoo, B S</au><au>Spenser, I D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The status of glycolaldehyde in the biosynthesis of vitamin B6</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1980-04-10</date><risdate>1980</risdate><volume>255</volume><issue>7</issue><spage>3042</spage><epage>3048</epage><pages>3042-3048</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Competition experiments, employing 14C-labeled samples of glycerol and glycolaldehyde, indicate that in Escherichia coli B
there are two independent pathways leading to pyridoxal. In mutant WG2 (and therefore presumably also in the wild strain)
the major pathway utilizes glycerol and related trioses as the sole carbon source in the construction of the C8N skeleton
of pyridoxol: C-1, -3 of glycerol yields C-2', -3, -4', -5' and -6, C-2 of glycerol yields C-2, -4, and -5 of the vitamin.
In the minor pathway glycolaldehyde and not glycerol supplies C-5 and C-5' of pyridoxol, while glycerol is the source of the
other 6 carbon atoms. In mutant WG3 the major route is blocked and the "glycolaldehyde pathway" becomes the sole source of
vitamin B6.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>6987231</pmid><doi>10.1016/S0021-9258(19)85849-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1980-04, Vol.255 (7), p.3042-3048 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75038509 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acetaldehyde - analogs & derivatives Acetaldehyde - metabolism Carbon Radioisotopes Escherichia coli - metabolism Glycerol - metabolism Isotope Labeling Mutation Pyridoxine - biosynthesis Species Specificity |
| title | The status of glycolaldehyde in the biosynthesis of vitamin B6 |
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