Comparison of potential nuclear precursors for prolactin and growth hormone messenger RNA
Recombinant DNA plasmids containing the coding sequence for rat prolactin or rat growth hormone have been used to investigate the presence of possible precursors for prolactin and growth hormone mRNA. Cytoplasmic and nuclear RNA was prepared from either rat pituitaries or fromthe GC pituitary cell l...
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| Veröffentlicht in: | The Journal of biological chemistry 1980-03, Vol.255 (6), p.2243-2246 |
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| container_title | The Journal of biological chemistry |
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| creator | Maurer, R A Gubbins, E J Erwin, C R Donelson, J E |
| description | Recombinant DNA plasmids containing the coding sequence for rat prolactin or rat growth hormone have been used to investigate
the presence of possible precursors for prolactin and growth hormone mRNA. Cytoplasmic and nuclear RNA was prepared from either
rat pituitaries or fromthe GC pituitary cell line. RNA was electrophoresed on agarose gels containing methylmercury hydroxide
and then transferred to diazobenzyloxymethyl paper. The paper was then hybridized to prolactin or growth hormone recombinant
DNA probes labeled in vitro with 32P. The prolactin probe hybridized to RNA species of 7.0, 6.4, 3.8, 1.7, and 1.0 kilobases
in nuclear RNA and only to a 1.0-kilobase species in cytoplasmic RNA. Hybridization with a growth hormone probe demonstrated
nuclear RNA species of 6.7, 5.6, 2.3, and 1.0 kilobases. These findings demonstrate the presence of multiple species of prolactin
and growth hormone RNA which are larger larger than the mature cytoplasmic mRNAs. The large nuclear RNAs are likely precursors
for prolactin and growth hormone mRNA. |
| doi_str_mv | 10.1016/S0021-9258(19)85878-7 |
| format | Article |
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the presence of possible precursors for prolactin and growth hormone mRNA. Cytoplasmic and nuclear RNA was prepared from either
rat pituitaries or fromthe GC pituitary cell line. RNA was electrophoresed on agarose gels containing methylmercury hydroxide
and then transferred to diazobenzyloxymethyl paper. The paper was then hybridized to prolactin or growth hormone recombinant
DNA probes labeled in vitro with 32P. The prolactin probe hybridized to RNA species of 7.0, 6.4, 3.8, 1.7, and 1.0 kilobases
in nuclear RNA and only to a 1.0-kilobase species in cytoplasmic RNA. Hybridization with a growth hormone probe demonstrated
nuclear RNA species of 6.7, 5.6, 2.3, and 1.0 kilobases. These findings demonstrate the presence of multiple species of prolactin
and growth hormone RNA which are larger larger than the mature cytoplasmic mRNAs. The large nuclear RNAs are likely precursors
for prolactin and growth hormone mRNA.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)85878-7</identifier><identifier>PMID: 6244273</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; Base Sequence ; Cell Nucleus - metabolism ; DNA Restriction Enzymes ; DNA, Recombinant - metabolism ; Female ; Growth Hormone - biosynthesis ; Molecular Weight ; Nucleic Acid Hybridization ; Pituitary Gland, Anterior - metabolism ; Plasmids ; Prolactin - biosynthesis ; Protein Biosynthesis ; Rats ; RNA, Messenger - metabolism</subject><ispartof>The Journal of biological chemistry, 1980-03, Vol.255 (6), p.2243-2246</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-26a863f7e59dfab6850815df372a5fc633d64a640fc7ef054d95cfe47eb760323</citedby><cites>FETCH-LOGICAL-c378t-26a863f7e59dfab6850815df372a5fc633d64a640fc7ef054d95cfe47eb760323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6244273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maurer, R A</creatorcontrib><creatorcontrib>Gubbins, E J</creatorcontrib><creatorcontrib>Erwin, C R</creatorcontrib><creatorcontrib>Donelson, J E</creatorcontrib><title>Comparison of potential nuclear precursors for prolactin and growth hormone messenger RNA</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Recombinant DNA plasmids containing the coding sequence for rat prolactin or rat growth hormone have been used to investigate
the presence of possible precursors for prolactin and growth hormone mRNA. Cytoplasmic and nuclear RNA was prepared from either
rat pituitaries or fromthe GC pituitary cell line. RNA was electrophoresed on agarose gels containing methylmercury hydroxide
and then transferred to diazobenzyloxymethyl paper. The paper was then hybridized to prolactin or growth hormone recombinant
DNA probes labeled in vitro with 32P. The prolactin probe hybridized to RNA species of 7.0, 6.4, 3.8, 1.7, and 1.0 kilobases
in nuclear RNA and only to a 1.0-kilobase species in cytoplasmic RNA. Hybridization with a growth hormone probe demonstrated
nuclear RNA species of 6.7, 5.6, 2.3, and 1.0 kilobases. These findings demonstrate the presence of multiple species of prolactin
and growth hormone RNA which are larger larger than the mature cytoplasmic mRNAs. The large nuclear RNAs are likely precursors
for prolactin and growth hormone mRNA.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Nucleus - metabolism</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Recombinant - metabolism</subject><subject>Female</subject><subject>Growth Hormone - biosynthesis</subject><subject>Molecular Weight</subject><subject>Nucleic Acid Hybridization</subject><subject>Pituitary Gland, Anterior - metabolism</subject><subject>Plasmids</subject><subject>Prolactin - biosynthesis</subject><subject>Protein Biosynthesis</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kElLxEAQhRtRxnH5CULjQfQQ7SW95CiDG4iCC-ip6elUTyJJeuxOEP-9mQXrUhTvvariQ-iEkktKqLx6JYTRrGBCn9PiQgutdKZ20JQSzTMu6Mcumv5b9tFBSl9krLygEzSRLM-Z4lP0OQvt0sY6hQ4Hj5ehh66vbYO7wTVgI15GcENMISbsw2oMjXV93WHblXgRw09f4SrENnSAW0gJugVE_PJ0fYT2vG0SHG_7IXq_vXmb3WePz3cPs-vHzHGl-4xJqyX3CkRRejuXWhBNRem5YlZ4JzkvZW5lTrxT4InIy0I4D7mCuZKEM36IzjZ7x9e-B0i9aevkoGlsB2FIRgnCBSmK0Sg2RhdDShG8Wca6tfHXUGJWSM0aqVnxMrQwa6RGjbmT7YFh3kL5n9oyHPXTjV7Vi-qnjmDmdXAVtIYJYaRhLOf8D4d_fnI</recordid><startdate>19800325</startdate><enddate>19800325</enddate><creator>Maurer, R A</creator><creator>Gubbins, E J</creator><creator>Erwin, C R</creator><creator>Donelson, J E</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800325</creationdate><title>Comparison of potential nuclear precursors for prolactin and growth hormone messenger RNA</title><author>Maurer, R A ; Gubbins, E J ; Erwin, C R ; Donelson, J E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-26a863f7e59dfab6850815df372a5fc633d64a640fc7ef054d95cfe47eb760323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cell Nucleus - metabolism</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Recombinant - metabolism</topic><topic>Female</topic><topic>Growth Hormone - biosynthesis</topic><topic>Molecular Weight</topic><topic>Nucleic Acid Hybridization</topic><topic>Pituitary Gland, Anterior - metabolism</topic><topic>Plasmids</topic><topic>Prolactin - biosynthesis</topic><topic>Protein Biosynthesis</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maurer, R A</creatorcontrib><creatorcontrib>Gubbins, E J</creatorcontrib><creatorcontrib>Erwin, C R</creatorcontrib><creatorcontrib>Donelson, J E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maurer, R A</au><au>Gubbins, E J</au><au>Erwin, C R</au><au>Donelson, J E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of potential nuclear precursors for prolactin and growth hormone messenger RNA</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1980-03-25</date><risdate>1980</risdate><volume>255</volume><issue>6</issue><spage>2243</spage><epage>2246</epage><pages>2243-2246</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Recombinant DNA plasmids containing the coding sequence for rat prolactin or rat growth hormone have been used to investigate
the presence of possible precursors for prolactin and growth hormone mRNA. Cytoplasmic and nuclear RNA was prepared from either
rat pituitaries or fromthe GC pituitary cell line. RNA was electrophoresed on agarose gels containing methylmercury hydroxide
and then transferred to diazobenzyloxymethyl paper. The paper was then hybridized to prolactin or growth hormone recombinant
DNA probes labeled in vitro with 32P. The prolactin probe hybridized to RNA species of 7.0, 6.4, 3.8, 1.7, and 1.0 kilobases
in nuclear RNA and only to a 1.0-kilobase species in cytoplasmic RNA. Hybridization with a growth hormone probe demonstrated
nuclear RNA species of 6.7, 5.6, 2.3, and 1.0 kilobases. These findings demonstrate the presence of multiple species of prolactin
and growth hormone RNA which are larger larger than the mature cytoplasmic mRNAs. The large nuclear RNAs are likely precursors
for prolactin and growth hormone mRNA.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>6244273</pmid><doi>10.1016/S0021-9258(19)85878-7</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1980-03, Vol.255 (6), p.2243-2246 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75035099 |
| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Base Sequence Cell Nucleus - metabolism DNA Restriction Enzymes DNA, Recombinant - metabolism Female Growth Hormone - biosynthesis Molecular Weight Nucleic Acid Hybridization Pituitary Gland, Anterior - metabolism Plasmids Prolactin - biosynthesis Protein Biosynthesis Rats RNA, Messenger - metabolism |
| title | Comparison of potential nuclear precursors for prolactin and growth hormone messenger RNA |
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