Construction of a SalI/ PstI restriction map of spinach chloroplast dna using low-gelling-temperature-agarose electrophoresis

The restriction endonucleases SalI and PstI cleave circular chloroplast DNA of spinach ( Spinacia oleracea) into 12 and 10 fragments, respectively. The sum of the fragment sizes in each of the series is equivalent to the contour length of the molecule (about 95 Md). A physical map was constructed by...

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Veröffentlicht in:	Gene 1980, Vol.8 (2), p.179-191
Hauptverfasser:	Herrmann, R.G., Whitfeld, P.R., Bottomley, W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Chloroplasts - analysis Chromosome Mapping DNA Restriction Enzymes - metabolism DNA, Circular - analysis Electrophoresis, Agar Gel inverted repeat, DNA digestion in melted agarose SalI, PstI and KpnI endonucleases Spinacia oleracea Vegetables
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