pMB9 plasmids bearing the Salmonella typhimurium his operon and gnd gene
A plasmid containing the entire Salmonella typhimurium his operon was constructed from plasmid pMB9 and an EcoRI fragment of φ80 his immλ DNA. The recombinant pST41 also includes the gluconate 6-phosphate dehydrogenase ( gnd) gene and has one EcoRI endonuclease cleavage site in the integrated fragme...
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| Veröffentlicht in: | Gene 1980-02, Vol.8 (3), p.239-253 |
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| creator | Bhaduri, S. Asai, T.K Schlessinger, D. Raskas, H.J. |
| description | A plasmid containing the entire
Salmonella typhimurium his operon was constructed from plasmid pMB9 and an
EcoRI fragment of φ80
his
immλ DNA. The recombinant pST41 also includes the gluconate 6-phosphate dehydrogenase (
gnd) gene and has one
EcoRI endonuclease cleavage site in the integrated fragment. This plasmid served as a source for the construction of two additional plasmids, one carrying the OGD
▪-region of the
his operon and the other a
▪
BHAFIE segment of the
his gene along with the
gnd gene. The presence of the
his operon in the constructed plasmids was confirmed by hybridization to
S. typhimurium his RNA. The location of the
gnd gene in the
▪
BHAFIE fragment of the
his gene was confirmed genetically: after transfection with the plasmid bearing the
gnd gene, a
gnd recipient gained the capacity to utilize gluconate as a sole carbon source. The DNAs of the three hybrid plasmids were analyzed by gel electrophoresis. By comparing the
EcoRI endonuclease cleavage pattern of these three hybrid plasmids with the DNA cleavage pattern of φ80
his
immλ, φ80
immλ and λ phages, the
EcoRI cleavage map of φ80
his
immλ was obtained. |
| doi_str_mv | 10.1016/0378-1119(80)90002-5 |
| format | Article |
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Salmonella typhimurium his operon was constructed from plasmid pMB9 and an
EcoRI fragment of φ80
his
immλ DNA. The recombinant pST41 also includes the gluconate 6-phosphate dehydrogenase (
gnd) gene and has one
EcoRI endonuclease cleavage site in the integrated fragment. This plasmid served as a source for the construction of two additional plasmids, one carrying the OGD
▪-region of the
his operon and the other a
▪
BHAFIE segment of the
his gene along with the
gnd gene. The presence of the
his operon in the constructed plasmids was confirmed by hybridization to
S. typhimurium his RNA. The location of the
gnd gene in the
▪
BHAFIE fragment of the
his gene was confirmed genetically: after transfection with the plasmid bearing the
gnd gene, a
gnd recipient gained the capacity to utilize gluconate as a sole carbon source. The DNAs of the three hybrid plasmids were analyzed by gel electrophoresis. By comparing the
EcoRI endonuclease cleavage pattern of these three hybrid plasmids with the DNA cleavage pattern of φ80
his
immλ, φ80
immλ and λ phages, the
EcoRI cleavage map of φ80
his
immλ was obtained.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(80)90002-5</identifier><identifier>PMID: 6244214</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bacteriophage lambda - genetics ; Chromosome Mapping ; Chromosomes, Bacterial ; DNA Restriction Enzymes - metabolism ; DNA, Recombinant - analysis ; DNA, Viral - genetics ; Electrophoresis, Agar Gel ; Histidine - genetics ; Operon ; phage φ80 hisimmλ ; Plasmids ; pMB9 plasmid ; Recombinant DNA ; restriction endonuclease Eco RI ; Salmonella typhimurium - genetics</subject><ispartof>Gene, 1980-02, Vol.8 (3), p.239-253</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-a822cfe297034726700ab0a95065efba602f3eb1eaa3b4ff9e0a80b31f697123</citedby><cites>FETCH-LOGICAL-c357t-a822cfe297034726700ab0a95065efba602f3eb1eaa3b4ff9e0a80b31f697123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(80)90002-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6244214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhaduri, S.</creatorcontrib><creatorcontrib>Asai, T.K</creatorcontrib><creatorcontrib>Schlessinger, D.</creatorcontrib><creatorcontrib>Raskas, H.J.</creatorcontrib><title>pMB9 plasmids bearing the Salmonella typhimurium his operon and gnd gene</title><title>Gene</title><addtitle>Gene</addtitle><description>A plasmid containing the entire
Salmonella typhimurium his operon was constructed from plasmid pMB9 and an
EcoRI fragment of φ80
his
immλ DNA. The recombinant pST41 also includes the gluconate 6-phosphate dehydrogenase (
gnd) gene and has one
EcoRI endonuclease cleavage site in the integrated fragment. This plasmid served as a source for the construction of two additional plasmids, one carrying the OGD
▪-region of the
his operon and the other a
▪
BHAFIE segment of the
his gene along with the
gnd gene. The presence of the
his operon in the constructed plasmids was confirmed by hybridization to
S. typhimurium his RNA. The location of the
gnd gene in the
▪
BHAFIE fragment of the
his gene was confirmed genetically: after transfection with the plasmid bearing the
gnd gene, a
gnd recipient gained the capacity to utilize gluconate as a sole carbon source. The DNAs of the three hybrid plasmids were analyzed by gel electrophoresis. By comparing the
EcoRI endonuclease cleavage pattern of these three hybrid plasmids with the DNA cleavage pattern of φ80
his
immλ, φ80
immλ and λ phages, the
EcoRI cleavage map of φ80
his
immλ was obtained.</description><subject>Bacteriophage lambda - genetics</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Bacterial</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>DNA, Recombinant - analysis</subject><subject>DNA, Viral - genetics</subject><subject>Electrophoresis, Agar Gel</subject><subject>Histidine - genetics</subject><subject>Operon</subject><subject>phage φ80 hisimmλ</subject><subject>Plasmids</subject><subject>pMB9 plasmid</subject><subject>Recombinant DNA</subject><subject>restriction endonuclease Eco RI</subject><subject>Salmonella typhimurium - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EKqXwByB5hWARGNt5eYMEFVCkIhZ0bznJuDXKCztB6t-T0KpLFqNZzL13Zg4hlwzuGLD4HkSSBowxeZPCrQQAHkRHZMrSRAYAIj0m04PklJx5_zVoIIr4hExiHoachVOyaN-fJG1L7StbeJqhdrZe026D9FOXVVNjWWrabduNrXpn-4purKdNi66pqa4Luh4LazwnJ0aXHi_2fUZWL8-r-SJYfry-zR-XQS6ipAt0ynlukMsERJjwOAHQGWgZQRyhyXQM3AjMGGotstAYiaBTyAQzsUwYFzNyvYttXfPdo-9UZX0-Hllj03uVRENuyNggDHfC3DXeOzSqdbbSbqsYqJGfGuGoEY5KQf3xU9Fgu9rn91mFxcG0BzbMH3ZzHH78seiUzy3WORbWYd6porH_L_gFJCZ-KQ</recordid><startdate>198002</startdate><enddate>198002</enddate><creator>Bhaduri, S.</creator><creator>Asai, T.K</creator><creator>Schlessinger, D.</creator><creator>Raskas, H.J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198002</creationdate><title>pMB9 plasmids bearing the Salmonella typhimurium his operon and gnd gene</title><author>Bhaduri, S. ; Asai, T.K ; Schlessinger, D. ; Raskas, H.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-a822cfe297034726700ab0a95065efba602f3eb1eaa3b4ff9e0a80b31f697123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Bacteriophage lambda - genetics</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Bacterial</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>DNA, Recombinant - analysis</topic><topic>DNA, Viral - genetics</topic><topic>Electrophoresis, Agar Gel</topic><topic>Histidine - genetics</topic><topic>Operon</topic><topic>phage φ80 hisimmλ</topic><topic>Plasmids</topic><topic>pMB9 plasmid</topic><topic>Recombinant DNA</topic><topic>restriction endonuclease Eco RI</topic><topic>Salmonella typhimurium - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhaduri, S.</creatorcontrib><creatorcontrib>Asai, T.K</creatorcontrib><creatorcontrib>Schlessinger, D.</creatorcontrib><creatorcontrib>Raskas, H.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhaduri, S.</au><au>Asai, T.K</au><au>Schlessinger, D.</au><au>Raskas, H.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>pMB9 plasmids bearing the Salmonella typhimurium his operon and gnd gene</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1980-02</date><risdate>1980</risdate><volume>8</volume><issue>3</issue><spage>239</spage><epage>253</epage><pages>239-253</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A plasmid containing the entire
Salmonella typhimurium his operon was constructed from plasmid pMB9 and an
EcoRI fragment of φ80
his
immλ DNA. The recombinant pST41 also includes the gluconate 6-phosphate dehydrogenase (
gnd) gene and has one
EcoRI endonuclease cleavage site in the integrated fragment. This plasmid served as a source for the construction of two additional plasmids, one carrying the OGD
▪-region of the
his operon and the other a
▪
BHAFIE segment of the
his gene along with the
gnd gene. The presence of the
his operon in the constructed plasmids was confirmed by hybridization to
S. typhimurium his RNA. The location of the
gnd gene in the
▪
BHAFIE fragment of the
his gene was confirmed genetically: after transfection with the plasmid bearing the
gnd gene, a
gnd recipient gained the capacity to utilize gluconate as a sole carbon source. The DNAs of the three hybrid plasmids were analyzed by gel electrophoresis. By comparing the
EcoRI endonuclease cleavage pattern of these three hybrid plasmids with the DNA cleavage pattern of φ80
his
immλ, φ80
immλ and λ phages, the
EcoRI cleavage map of φ80
his
immλ was obtained.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6244214</pmid><doi>10.1016/0378-1119(80)90002-5</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1980-02, Vol.8 (3), p.239-253 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75034411 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacteriophage lambda - genetics Chromosome Mapping Chromosomes, Bacterial DNA Restriction Enzymes - metabolism DNA, Recombinant - analysis DNA, Viral - genetics Electrophoresis, Agar Gel Histidine - genetics Operon phage φ80 hisimmλ Plasmids pMB9 plasmid Recombinant DNA restriction endonuclease Eco RI Salmonella typhimurium - genetics |
| title | pMB9 plasmids bearing the Salmonella typhimurium his operon and gnd gene |
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