Human Rotavirus Type 2: Cultivation in vitro
A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus pre...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1980-01, Vol.207 (4427), p.189-191 |
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| container_end_page | 191 |
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| container_issue | 4427 |
| container_start_page | 189 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 207 |
| creator | Wyatt, Richard G. James, Walter D. Bohl, Edward H. Theil, Kenneth W. Saif, Linda J. Kalica, Anthony R. Greenberg, Harry B. Kapikian, Albert Z. Chanock, Robert M. |
| description | A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus present in the stool of patient Wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in African green monkey kidney cells. Prior to each passage in cell culture, the virus was treated with trypsin and the inoculated cultures were centrifuged at low speed. Cultivation of a type 2 human rotavirus should aid attempts to characterize this virus and to develop a means of immunoprophylaxis for a serious diarrheal disease of human infants. |
| doi_str_mv | 10.1126/science.6243190 |
| format | Article |
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This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus present in the stool of patient Wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in African green monkey kidney cells. Prior to each passage in cell culture, the virus was treated with trypsin and the inoculated cultures were centrifuged at low speed. Cultivation of a type 2 human rotavirus should aid attempts to characterize this virus and to develop a means of immunoprophylaxis for a serious diarrheal disease of human infants.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.6243190</identifier><identifier>PMID: 6243190</identifier><language>eng</language><publisher>United States: The American Association for the Advancement of Science</publisher><subject>Animals ; Antigens, Viral - analysis ; Cells, Cultured ; Cultured cells ; Diarrhea, Infantile - microbiology ; Gastrointestinal secretions ; Germ-Free Life ; Gnotobiotics ; Haplorhini ; Humans ; Infant ; Infections ; Kidney cells ; RNA ; RNA Viruses - growth & development ; Rotavirus ; Rotavirus - growth & development ; Rotavirus - immunology ; Swine ; Ungulates ; Viruses</subject><ispartof>Science (American Association for the Advancement of Science), 1980-01, Vol.207 (4427), p.189-191</ispartof><rights>Copyright 1980 The American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c324t-d02b0d0cd8ccad95a20987d85fcd5ad7bfbfe97ef2b6b88916826525f101bfc83</citedby><cites>FETCH-LOGICAL-c324t-d02b0d0cd8ccad95a20987d85fcd5ad7bfbfe97ef2b6b88916826525f101bfc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1683908$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1683908$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,2871,2872,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6243190$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wyatt, Richard G.</creatorcontrib><creatorcontrib>James, Walter D.</creatorcontrib><creatorcontrib>Bohl, Edward H.</creatorcontrib><creatorcontrib>Theil, Kenneth W.</creatorcontrib><creatorcontrib>Saif, Linda J.</creatorcontrib><creatorcontrib>Kalica, Anthony R.</creatorcontrib><creatorcontrib>Greenberg, Harry B.</creatorcontrib><creatorcontrib>Kapikian, Albert Z.</creatorcontrib><creatorcontrib>Chanock, Robert M.</creatorcontrib><title>Human Rotavirus Type 2: Cultivation in vitro</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus present in the stool of patient Wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in African green monkey kidney cells. Prior to each passage in cell culture, the virus was treated with trypsin and the inoculated cultures were centrifuged at low speed. Cultivation of a type 2 human rotavirus should aid attempts to characterize this virus and to develop a means of immunoprophylaxis for a serious diarrheal disease of human infants.</description><subject>Animals</subject><subject>Antigens, Viral - analysis</subject><subject>Cells, Cultured</subject><subject>Cultured cells</subject><subject>Diarrhea, Infantile - microbiology</subject><subject>Gastrointestinal secretions</subject><subject>Germ-Free Life</subject><subject>Gnotobiotics</subject><subject>Haplorhini</subject><subject>Humans</subject><subject>Infant</subject><subject>Infections</subject><subject>Kidney cells</subject><subject>RNA</subject><subject>RNA Viruses - growth & development</subject><subject>Rotavirus</subject><subject>Rotavirus - growth & development</subject><subject>Rotavirus - immunology</subject><subject>Swine</subject><subject>Ungulates</subject><subject>Viruses</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEUhYMotVbXbhRm5cppb5JmJnFXilqhIEhdhzwhZR41mSn031vpYFd38X3nwD0I3WOYYkyKWTLBNcZNCzKnWMAFGmMQLBcE6CUaA9Ai51Cya3ST0hbgyAQdodGgj9Hzqq9Vk321ndqH2Kdsc9i5jLxky77qwl51oW2y0GT70MX2Fl15VSV3N9wJ-n573SxX-frz_WO5WOeGknmXWyAaLBjLjVFWMEVA8NJy5o1lypbaa-9E6TzRheZc4IKTghHmMWDtDacT9HTq3cX2p3epk3VIxlWValzbJ1kywJRBeRRnJ9HENqXovNzFUKt4kBjk3z5y2EcODx8Tj0N1r2tn__0zfzjxberaeK4rOBXA6S-EaWsH</recordid><startdate>19800111</startdate><enddate>19800111</enddate><creator>Wyatt, Richard G.</creator><creator>James, Walter D.</creator><creator>Bohl, Edward H.</creator><creator>Theil, Kenneth W.</creator><creator>Saif, Linda J.</creator><creator>Kalica, Anthony R.</creator><creator>Greenberg, Harry B.</creator><creator>Kapikian, Albert Z.</creator><creator>Chanock, Robert M.</creator><general>The American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800111</creationdate><title>Human Rotavirus Type 2: Cultivation in vitro</title><author>Wyatt, Richard G. ; James, Walter D. ; Bohl, Edward H. ; Theil, Kenneth W. ; Saif, Linda J. ; Kalica, Anthony R. ; Greenberg, Harry B. ; Kapikian, Albert Z. ; Chanock, Robert M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c324t-d02b0d0cd8ccad95a20987d85fcd5ad7bfbfe97ef2b6b88916826525f101bfc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Antigens, Viral - analysis</topic><topic>Cells, Cultured</topic><topic>Cultured cells</topic><topic>Diarrhea, Infantile - microbiology</topic><topic>Gastrointestinal secretions</topic><topic>Germ-Free Life</topic><topic>Gnotobiotics</topic><topic>Haplorhini</topic><topic>Humans</topic><topic>Infant</topic><topic>Infections</topic><topic>Kidney cells</topic><topic>RNA</topic><topic>RNA Viruses - growth & development</topic><topic>Rotavirus</topic><topic>Rotavirus - growth & development</topic><topic>Rotavirus - immunology</topic><topic>Swine</topic><topic>Ungulates</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wyatt, Richard G.</creatorcontrib><creatorcontrib>James, Walter D.</creatorcontrib><creatorcontrib>Bohl, Edward H.</creatorcontrib><creatorcontrib>Theil, Kenneth W.</creatorcontrib><creatorcontrib>Saif, Linda J.</creatorcontrib><creatorcontrib>Kalica, Anthony R.</creatorcontrib><creatorcontrib>Greenberg, Harry B.</creatorcontrib><creatorcontrib>Kapikian, Albert Z.</creatorcontrib><creatorcontrib>Chanock, Robert M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wyatt, Richard G.</au><au>James, Walter D.</au><au>Bohl, Edward H.</au><au>Theil, Kenneth W.</au><au>Saif, Linda J.</au><au>Kalica, Anthony R.</au><au>Greenberg, Harry B.</au><au>Kapikian, Albert Z.</au><au>Chanock, Robert M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Rotavirus Type 2: Cultivation in vitro</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1980-01-11</date><risdate>1980</risdate><volume>207</volume><issue>4427</issue><spage>189</spage><epage>191</epage><pages>189-191</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. In contrast, virus present in the stool of patient Wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in African green monkey kidney cells. Prior to each passage in cell culture, the virus was treated with trypsin and the inoculated cultures were centrifuged at low speed. Cultivation of a type 2 human rotavirus should aid attempts to characterize this virus and to develop a means of immunoprophylaxis for a serious diarrheal disease of human infants.</abstract><cop>United States</cop><pub>The American Association for the Advancement of Science</pub><pmid>6243190</pmid><doi>10.1126/science.6243190</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1980-01, Vol.207 (4427), p.189-191 |
| issn | 0036-8075 1095-9203 |
| language | eng |
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| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Animals Antigens, Viral - analysis Cells, Cultured Cultured cells Diarrhea, Infantile - microbiology Gastrointestinal secretions Germ-Free Life Gnotobiotics Haplorhini Humans Infant Infections Kidney cells RNA RNA Viruses - growth & development Rotavirus Rotavirus - growth & development Rotavirus - immunology Swine Ungulates Viruses |
| title | Human Rotavirus Type 2: Cultivation in vitro |
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