Exposure of Hidden Antigenic Determinants in Human IgG by Digestion with Spleen Proteases

Fragments of human IgG produced by digestion with spleen proteases were tested for capacity to react with “agglutionators” present in human sera. Antigenic sites, apparently buried in the intact IgG, were exposed by these enzymes. Hemagglutination-inhibition tests indicated that the fragments produc...

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Veröffentlicht in:	The Journal of immunology (1950) 1971-09, Vol.107 (3), p.814-821
Hauptverfasser:	Fehr, Kurt, LoSpalluto, Joseph
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens - analysis Binding Sites Chromatography, Gel Epitopes - analysis Erythrocytes - immunology Hemagglutination Inhibition Tests Hemagglutination Tests Humans Immune Sera - analysis Immunochemistry Immunodiffusion Immunoglobulin G - analysis Immunoglobulin M - isolation & purification Lysosomes - enzymology Papain - pharmacology Pepsin A - pharmacology Peptide Hydrolases - pharmacology Rheumatoid Factor - analysis Spleen - enzymology Synovial Fluid - immunology
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Zusammenfassung:	Fragments of human IgG produced by digestion with spleen proteases were tested for capacity to react with “agglutionators” present in human sera. Antigenic sites, apparently buried in the intact IgG, were exposed by these enzymes. Hemagglutination-inhibition tests indicated that the fragments produced were similar to but not identical with those produced by digestion with pepsin or papain.
ISSN:	0022-1767 1550-6606
DOI:	10.4049/jimmunol.107.3.814