Isolation of myosin messenger ribonucleoprotein particles which contain a protein fraction affecting myosin synthesis
Myosin mRNP particles were purified using metrizamide buoyant density gradient centrifugation. This mRNP particle contains only the 26S myosin mRNA and, therefore, is a purified mRNP with regard to mRNA composition. A protein fraction, eluting from DEAE-cellulose between 0.15M KC l and 0.28M KC l ,...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1980-01, Vol.92 (2), p.524-531 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Bester, AndréJ Durrheim, Glenda Kennedy, Doris S Heywood, Stuart M |
| description | Myosin mRNP particles were purified using metrizamide buoyant density gradient centrifugation. This mRNP particle contains only the 26S myosin mRNA and, therefore, is a purified mRNP with regard to mRNA composition. A protein fraction, eluting from DEAE-cellulose between 0.15M KC
l
and 0.28M KC
l
, was iso=lated from the purified myosin mRNP particle. A significant stimulation of myosin heavy chain synthesis is observed when the isolated fraction is added to an unfractionated wheat germ cell-free system containing myosin mRNA. This protein fraction was found to have no effect on the translation of TMV RNA. In addition, this protein fraction does not bind TMV RNA, but is associated with myosin mRNA during translation. |
| doi_str_mv | 10.1016/0006-291X(80)90365-4 |
| format | Article |
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l
and 0.28M KC
l
, was iso=lated from the purified myosin mRNP particle. A significant stimulation of myosin heavy chain synthesis is observed when the isolated fraction is added to an unfractionated wheat germ cell-free system containing myosin mRNA. This protein fraction was found to have no effect on the translation of TMV RNA. In addition, this protein fraction does not bind TMV RNA, but is associated with myosin mRNA during translation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(80)90365-4</identifier><identifier>PMID: 7356480</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chick Embryo ; Muscles - metabolism ; Myosins - biosynthesis ; Nucleoproteins - metabolism ; Plants - metabolism ; Protein Biosynthesis ; Ribonucleoproteins - metabolism ; Ribosomes - metabolism ; RNA, Messenger - metabolism ; Triticum - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1980-01, Vol.92 (2), p.524-531</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-b2843d63499fba633fb00eaa4be116f63c892330cff48e2ac80acc451cc41a7b3</citedby><cites>FETCH-LOGICAL-c376t-b2843d63499fba633fb00eaa4be116f63c892330cff48e2ac80acc451cc41a7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X80903654$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7356480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bester, AndréJ</creatorcontrib><creatorcontrib>Durrheim, Glenda</creatorcontrib><creatorcontrib>Kennedy, Doris S</creatorcontrib><creatorcontrib>Heywood, Stuart M</creatorcontrib><title>Isolation of myosin messenger ribonucleoprotein particles which contain a protein fraction affecting myosin synthesis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Myosin mRNP particles were purified using metrizamide buoyant density gradient centrifugation. This mRNP particle contains only the 26S myosin mRNA and, therefore, is a purified mRNP with regard to mRNA composition. A protein fraction, eluting from DEAE-cellulose between 0.15M KC
l
and 0.28M KC
l
, was iso=lated from the purified myosin mRNP particle. A significant stimulation of myosin heavy chain synthesis is observed when the isolated fraction is added to an unfractionated wheat germ cell-free system containing myosin mRNA. This protein fraction was found to have no effect on the translation of TMV RNA. In addition, this protein fraction does not bind TMV RNA, but is associated with myosin mRNA during translation.</description><subject>Animals</subject><subject>Chick Embryo</subject><subject>Muscles - metabolism</subject><subject>Myosins - biosynthesis</subject><subject>Nucleoproteins - metabolism</subject><subject>Plants - metabolism</subject><subject>Protein Biosynthesis</subject><subject>Ribonucleoproteins - metabolism</subject><subject>Ribosomes - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Triticum - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9r3DAQxUVoSTdJv0BowKeQHtyMLFm2L4EQ-icQ6KEN9CZk7WhXwZa2Grtlv3212U2OvWjEvDdP4jeMfeDwiQNX1wCgyqrjv65a-NiBUHUpj9iCQwdlxUG-YYtXyzt2QvQEwLlU3TE7bkStZAsLNt9THMzkYyiiK8ZtJB-KEYkwrDAVyfcxzHbAuElxwqxtTJp8blDxd-3turAxTCb3TfHicMnY50DjHOZbWL3k0jZMayRPZ-ytMwPh-0M9ZY9fPv-8-1Y-fP96f3f7UFrRqKnsq1aKpRKy61xvlBCuB0BjZI-cK6eEbbtKCLDOyRYrY1sw1sqa54Obphen7HKfm__2e0aa9OjJ4jCYgHEm3eTkuulENsq90aZIlNDpTfKjSVvNQe9o6x1KvUOpW9DPtLXMYxeH_Lkfcfk6dMCb9fO97kzUZpU86ccfbV6D4nUWb_YiZgJ_PCZN1mOwuPQpc9PL6P__-j-yPZqm</recordid><startdate>19800129</startdate><enddate>19800129</enddate><creator>Bester, AndréJ</creator><creator>Durrheim, Glenda</creator><creator>Kennedy, Doris S</creator><creator>Heywood, Stuart M</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19800129</creationdate><title>Isolation of myosin messenger ribonucleoprotein particles which contain a protein fraction affecting myosin synthesis</title><author>Bester, AndréJ ; Durrheim, Glenda ; Kennedy, Doris S ; Heywood, Stuart M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-b2843d63499fba633fb00eaa4be116f63c892330cff48e2ac80acc451cc41a7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Animals</topic><topic>Chick Embryo</topic><topic>Muscles - metabolism</topic><topic>Myosins - biosynthesis</topic><topic>Nucleoproteins - metabolism</topic><topic>Plants - metabolism</topic><topic>Protein Biosynthesis</topic><topic>Ribonucleoproteins - metabolism</topic><topic>Ribosomes - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Triticum - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bester, AndréJ</creatorcontrib><creatorcontrib>Durrheim, Glenda</creatorcontrib><creatorcontrib>Kennedy, Doris S</creatorcontrib><creatorcontrib>Heywood, Stuart M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bester, AndréJ</au><au>Durrheim, Glenda</au><au>Kennedy, Doris S</au><au>Heywood, Stuart M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of myosin messenger ribonucleoprotein particles which contain a protein fraction affecting myosin synthesis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1980-01-29</date><risdate>1980</risdate><volume>92</volume><issue>2</issue><spage>524</spage><epage>531</epage><pages>524-531</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Myosin mRNP particles were purified using metrizamide buoyant density gradient centrifugation. This mRNP particle contains only the 26S myosin mRNA and, therefore, is a purified mRNP with regard to mRNA composition. A protein fraction, eluting from DEAE-cellulose between 0.15M KC
l
and 0.28M KC
l
, was iso=lated from the purified myosin mRNP particle. A significant stimulation of myosin heavy chain synthesis is observed when the isolated fraction is added to an unfractionated wheat germ cell-free system containing myosin mRNA. This protein fraction was found to have no effect on the translation of TMV RNA. In addition, this protein fraction does not bind TMV RNA, but is associated with myosin mRNA during translation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7356480</pmid><doi>10.1016/0006-291X(80)90365-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1980-01, Vol.92 (2), p.524-531 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Chick Embryo Muscles - metabolism Myosins - biosynthesis Nucleoproteins - metabolism Plants - metabolism Protein Biosynthesis Ribonucleoproteins - metabolism Ribosomes - metabolism RNA, Messenger - metabolism Triticum - metabolism |
| title | Isolation of myosin messenger ribonucleoprotein particles which contain a protein fraction affecting myosin synthesis |
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