Characterization of promoter containing DNA fragments based on the abortive initiation reaction of Escherichia coli RNA polymerase

The abortive initiation reaction of Escherichia coli RNA polymerase was demonstrated to be a general method for the rapid identification and quantitation of promoter sites on DNA. The presence of the T7 promoters, A1, A2, A3, and D on an isolated restriction fragment of the phage template was demons...

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Veröffentlicht in:	The Journal of biological chemistry 1980-03, Vol.255 (5), p.1763-1766
Hauptverfasser:	Cech, C L, Lichy, J, McClure, W R
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA, Viral DNA-Directed RNA Polymerases - metabolism Escherichia coli - enzymology Microscopy, Electron Operon Protein Binding T-Phages Transcription, Genetic
Online-Zugang:	Volltext
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