Presence of a carcinoma-associated antigen(s) in the spent chemically defined medium of a human colon carcinoma cell line

Presence of a carcinoma-associated antigen(s) in the spent chemically defined medium of a human colon carcinoma cell line

The human colon carcinoma cell line HT‐29 was adapted to grow in chemically defined medium (CDM). The spent CDM (S‐CDM) was concentrated by Amicon filtration and the crude HT‐29 S‐CDM purified by 40% saturated(NH4)2 SO4 precipitation. The purified antigen was tested by a micro‐complement fixation (M...

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Veröffentlicht in:Journal of surgical oncology 1980-01, Vol.13 (1), p.45-51
Hauptverfasser: Chee, Darwin O., Gupta, Rishab K., Morton, Donald L.
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies, Neoplasm
antigen (s)
Antigens, Neoplasm
Breast Neoplasms - immunology
Cell Line
Colonic Neoplasms - immunology
Cross Reactions
Culture Media
Epitopes
Female
HT-29 cell line
Humans
Melanoma - immunology
Sarcoma - immunology
spent medium
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container_end_page 51
container_issue 1
container_start_page 45
container_title Journal of surgical oncology
container_volume 13
creator Chee, Darwin O.
Gupta, Rishab K.
Morton, Donald L.
description The human colon carcinoma cell line HT‐29 was adapted to grow in chemically defined medium (CDM). The spent CDM (S‐CDM) was concentrated by Amicon filtration and the crude HT‐29 S‐CDM purified by 40% saturated(NH4)2 SO4 precipitation. The purified antigen was tested by a micro‐complement fixation (MCF) assay against the sera of cancer patients of various histologic types and against the sera of normal donors. Fifteen of 20 (75%) colon cancer, 16/20 (80%) breast cancer sera, 14/19 (74%) lung cancer sera, and 13/20 (65%) miscellaneous carcinoma sera were positive in the MCF. By contrast, 2/21 (10%) melanoma sera, 7/20 (35%) sarcoma sera, and 2/19 (11 %) normal sera were positive. These data suggest the presence of a carcinoma‐associated antigen in the spent CDM of the HT‐29 colon carcinoma cell line adapted to grow in CDM.
doi_str_mv 10.1002/jso.2930130108
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Surg. Oncol</addtitle><description>The human colon carcinoma cell line HT‐29 was adapted to grow in chemically defined medium (CDM). The spent CDM (S‐CDM) was concentrated by Amicon filtration and the crude HT‐29 S‐CDM purified by 40% saturated(NH4)2 SO4 precipitation. The purified antigen was tested by a micro‐complement fixation (MCF) assay against the sera of cancer patients of various histologic types and against the sera of normal donors. Fifteen of 20 (75%) colon cancer, 16/20 (80%) breast cancer sera, 14/19 (74%) lung cancer sera, and 13/20 (65%) miscellaneous carcinoma sera were positive in the MCF. By contrast, 2/21 (10%) melanoma sera, 7/20 (35%) sarcoma sera, and 2/19 (11 %) normal sera were positive. 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Surg. Oncol</addtitle><date>1980-01</date><risdate>1980</risdate><volume>13</volume><issue>1</issue><spage>45</spage><epage>51</epage><pages>45-51</pages><issn>0022-4790</issn><eissn>1096-9098</eissn><abstract>The human colon carcinoma cell line HT‐29 was adapted to grow in chemically defined medium (CDM). The spent CDM (S‐CDM) was concentrated by Amicon filtration and the crude HT‐29 S‐CDM purified by 40% saturated(NH4)2 SO4 precipitation. The purified antigen was tested by a micro‐complement fixation (MCF) assay against the sera of cancer patients of various histologic types and against the sera of normal donors. Fifteen of 20 (75%) colon cancer, 16/20 (80%) breast cancer sera, 14/19 (74%) lung cancer sera, and 13/20 (65%) miscellaneous carcinoma sera were positive in the MCF. By contrast, 2/21 (10%) melanoma sera, 7/20 (35%) sarcoma sera, and 2/19 (11 %) normal sera were positive. These data suggest the presence of a carcinoma‐associated antigen in the spent CDM of the HT‐29 colon carcinoma cell line adapted to grow in CDM.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>6153228</pmid><doi>10.1002/jso.2930130108</doi><tpages>7</tpages></addata></record>
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source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects Antibodies, Neoplasm
antigen (s)
Antigens, Neoplasm
Breast Neoplasms - immunology
Cell Line
Colonic Neoplasms - immunology
Cross Reactions
Culture Media
Epitopes
Female
HT-29 cell line
Humans
Melanoma - immunology
Sarcoma - immunology
spent medium
title Presence of a carcinoma-associated antigen(s) in the spent chemically defined medium of a human colon carcinoma cell line
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