Characterization of alpha-1-antitrypsin by isoelectric focusing on an ultrathin polyacrylamide gel layer. An economic high-resolution system for determining PiM subtypes

Characterization of alpha-1-antitrypsin by isoelectric focusing on an ultrathin polyacrylamide gel layer. An economic high-resolution system for determining PiM subtypes

Through the use of ultrathin layer polyacrylamide gel isoelectric focusing it is possible to obtain a resolution of the bands of alpha 1AT so as to be able to easily recognize all six PiM subtypes. The optimal resolution of the PiM subtypes is obtained without deforming the pattern of the Pi phenoty...

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Veröffentlicht in:Human genetics 1979-01, Vol.53 (1), p.91-95
Hauptverfasser: Massi, G, Fabiano, A, Ragusa, D, Auconi, P
Format: Artikel
Sprache:eng
Schlagworte:
Adult
alpha 1-Antitrypsin - analysis
alpha 1-Antitrypsin - genetics
Ampholyte Mixtures
Female
Humans
Infant, Newborn
Isoelectric Focusing - methods
Male
Phenotype
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container_title Human genetics
container_volume 53
creator Massi, G
Fabiano, A
Ragusa, D
Auconi, P
description Through the use of ultrathin layer polyacrylamide gel isoelectric focusing it is possible to obtain a resolution of the bands of alpha 1AT so as to be able to easily recognize all six PiM subtypes. The optimal resolution of the PiM subtypes is obtained without deforming the pattern of the Pi phenotypes. In addition to high resolution, ultrathin layer polyacrylamide gel isoelectric focusing permits a notable reduction of fees to 1/5 of the usual.
doi_str_mv 10.1007/BF00289456
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source MEDLINE; SpringerLink Journals - AutoHoldings
subjects Adult
alpha 1-Antitrypsin - analysis
alpha 1-Antitrypsin - genetics
Ampholyte Mixtures
Female
Humans
Infant, Newborn
Isoelectric Focusing - methods
Male
Phenotype
title Characterization of alpha-1-antitrypsin by isoelectric focusing on an ultrathin polyacrylamide gel layer. An economic high-resolution system for determining PiM subtypes
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