Characterization of normal human cells by pyrolysis gas chromatography mass spectrometry
Differentiation of normal human cells has been accomplished by pyrolysis gas chromatography mass spectrometry. Normal cells from human kidney, spleen, liver and brain tissues have been pyrolyzed and the products chromatographically separated and characterized by mass spectrometry. Molecular pyrolysi...
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| Veröffentlicht in: | Biological Mass Spectrometry 1979-11, Vol.6 (11), p.491-498 |
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| container_issue | 11 |
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| container_title | Biological Mass Spectrometry |
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| creator | Reiner, E. Abbey, L. E. Moran, T. F. Papamichalis, P. Schafer, R. W. |
| description | Differentiation of normal human cells has been accomplished by pyrolysis gas chromatography mass spectrometry. Normal cells from human kidney, spleen, liver and brain tissues have been pyrolyzed and the products chromatographically separated and characterized by mass spectrometry. Molecular pyrolysis products giving rise to the characteristic pyro‐mass chromatograms include, but are not limited to, alkenes, alkanes, nitriles and various ring compounds. Single ion mass chromatograms as well as multiple ion mass chromatograms have been used to explore the characteristic differences between various tissue materials. A dynamic computer methodology for comparing pyro‐mass chromatograms has been developed for use in automatic identification and classification of the human cellular material. |
| doi_str_mv | 10.1002/bms.1200061107 |
| format | Article |
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E.</creatorcontrib><creatorcontrib>Moran, T. F.</creatorcontrib><creatorcontrib>Papamichalis, P.</creatorcontrib><creatorcontrib>Schafer, R. W.</creatorcontrib><title>Characterization of normal human cells by pyrolysis gas chromatography mass spectrometry</title><title>Biological Mass Spectrometry</title><addtitle>Biol. Mass Spectrom</addtitle><description>Differentiation of normal human cells has been accomplished by pyrolysis gas chromatography mass spectrometry. Normal cells from human kidney, spleen, liver and brain tissues have been pyrolyzed and the products chromatographically separated and characterized by mass spectrometry. Molecular pyrolysis products giving rise to the characteristic pyro‐mass chromatograms include, but are not limited to, alkenes, alkanes, nitriles and various ring compounds. Single ion mass chromatograms as well as multiple ion mass chromatograms have been used to explore the characteristic differences between various tissue materials. A dynamic computer methodology for comparing pyro‐mass chromatograms has been developed for use in automatic identification and classification of the human cellular material.</description><subject>Brain - cytology</subject><subject>Brain Chemistry</subject><subject>Computers</subject><subject>Cytological Techniques</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>Humans</subject><subject>Kidney - analysis</subject><subject>Kidney - cytology</subject><subject>Liver - analysis</subject><subject>Liver - cytology</subject><subject>Spleen - analysis</subject><subject>Spleen - cytology</subject><issn>0306-042X</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFP2zAYxS20wQrbdScOPu2W8jl27Pg4KtohATvQCW6W6zhtIKmDv1Rb9teTKhUTp50-6Xvv_fT0CPnKYMoA0otVg1OWAoBkDNQRmTDQMtF5nn8gE-AgExDp4ydyivgEwHMG_IQccy2UzCfkcbax0brOx-qv7aqwpaGk2xAbW9PNrrFb6nxdI131tO1jqHuskK4tUreJobFdWEfbbnraWESKrXfd8PZd7D-Tj6Wt0X853DPya361nP1Ibn4urmffbxLHlVKJ0xxSobMic44x6cVQvvRKOJkWupTAsqwovNBFuRLFYJGKeckUsIL7XEPKz8i3kdvG8LLz2Jmmwn1nu_Vhh0YJDdlAHozT0ehiQIy-NG2sGht7w8DslzTDkubfkkPg_EDerRpfvNnH6QZZj_Lvqvb9f2Dm8vb-HToZsxV2_s9b1sZnIxVXmXm4Wxgxf5DLpbozjL8CeY2Prw</recordid><startdate>197911</startdate><enddate>197911</enddate><creator>Reiner, E.</creator><creator>Abbey, L. 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Mass Spectrom</addtitle><date>1979-11</date><risdate>1979</risdate><volume>6</volume><issue>11</issue><spage>491</spage><epage>498</epage><pages>491-498</pages><issn>0306-042X</issn><eissn>1096-9888</eissn><abstract>Differentiation of normal human cells has been accomplished by pyrolysis gas chromatography mass spectrometry. Normal cells from human kidney, spleen, liver and brain tissues have been pyrolyzed and the products chromatographically separated and characterized by mass spectrometry. Molecular pyrolysis products giving rise to the characteristic pyro‐mass chromatograms include, but are not limited to, alkenes, alkanes, nitriles and various ring compounds. Single ion mass chromatograms as well as multiple ion mass chromatograms have been used to explore the characteristic differences between various tissue materials. A dynamic computer methodology for comparing pyro‐mass chromatograms has been developed for use in automatic identification and classification of the human cellular material.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>394768</pmid><doi>10.1002/bms.1200061107</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biological Mass Spectrometry, 1979-11, Vol.6 (11), p.491-498 |
| issn | 0306-042X 1096-9888 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74905116 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Brain - cytology Brain Chemistry Computers Cytological Techniques Gas Chromatography-Mass Spectrometry - methods Humans Kidney - analysis Kidney - cytology Liver - analysis Liver - cytology Spleen - analysis Spleen - cytology |
| title | Characterization of normal human cells by pyrolysis gas chromatography mass spectrometry |
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