Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles
The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4 nM citrate-capped...
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| Veröffentlicht in: | Talanta (Oxford) 2010-07, Vol.82 (2), p.693-697 |
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| creator | Wang, Xinyi Xu, Yao Xu, Xiao Hu, Ke Xiang, Minghui Li, Limei Liu, Feng Li, Na |
| description | The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4
nM citrate-capped AuNPs (13
nm in diameter), the linear range of the calibration curve was 15-50
nM with a detection limit of 13.1
nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination. |
| doi_str_mv | 10.1016/j.talanta.2010.05.034 |
| format | Article |
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nM citrate-capped AuNPs (13
nm in diameter), the linear range of the calibration curve was 15-50
nM with a detection limit of 13.1
nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2010.05.034</identifier><identifier>PMID: 20602956</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Chemistry ; Exact sciences and technology ; Gold - analysis ; Gold - chemistry ; Gold Nanoparticles ; Humans ; Limit of Detection ; Lysozyme ; Metal Nanoparticles - chemistry ; Microscopy, Electron, Transmission ; Muramidase - urine ; Plasmon Resonance Light-Scattering ; Spectrometric and optical methods ; Surface Plasmon Resonance - methods ; Urinary sample</subject><ispartof>Talanta (Oxford), 2010-07, Vol.82 (2), p.693-697</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-be22e518ad62f26754a9e57acd46b321b3c6cb0cdd215a585d6f8bfbc13dff193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2010.05.034$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23075491$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20602956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xinyi</creatorcontrib><creatorcontrib>Xu, Yao</creatorcontrib><creatorcontrib>Xu, Xiao</creatorcontrib><creatorcontrib>Hu, Ke</creatorcontrib><creatorcontrib>Xiang, Minghui</creatorcontrib><creatorcontrib>Li, Limei</creatorcontrib><creatorcontrib>Liu, Feng</creatorcontrib><creatorcontrib>Li, Na</creatorcontrib><title>Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4
nM citrate-capped AuNPs (13
nm in diameter), the linear range of the calibration curve was 15-50
nM with a detection limit of 13.1
nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination.</description><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Gold - analysis</subject><subject>Gold - chemistry</subject><subject>Gold Nanoparticles</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Lysozyme</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microscopy, Electron, Transmission</subject><subject>Muramidase - urine</subject><subject>Plasmon Resonance Light-Scattering</subject><subject>Spectrometric and optical methods</subject><subject>Surface Plasmon Resonance - methods</subject><subject>Urinary sample</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO0zAQhi0EYsvCI4ByQXtKGdtx0pwQ2oUFqQLE0rPl2OPFVRIX20UqT8-UFjhysjz65vfMZ8aec1hy4O2r7bKY0czFLAVQDdQSZPOALfiqk7VUnXzIFgCyr3vewAV7kvMWAIQE-ZhdCGhB9KpdsHgTEtpS3WDBNIXZlBDnKvpqk-iSDtX6kOPPw4TVJof5vrrbJ28sVp9Hkyciv2COs5mpsg7330p9Z02hpCNKIbdxdNVHM8edSSXYEfNT9sibMeOz83nJNu_efr1-X68_3X64frOureybUg8oBCq-Mq4VXrSdakyPqjPWNe0gBR-kbe0A1jnBlVEr5Vq_GvxguXTe815esqtT7i7F73vMRU8hWxxJGcZ91l3Tg-gUF0SqE2lTzDmh17sUJlpdc9BH1Xqrz6r1UbUGpUk19b04v7AfJnR_u_64JeDlGTDZmtEn0hTyP04CrdVz4l6fOCQfPwImnW1AUup-f412MfxnlF9quaFb</recordid><startdate>20100715</startdate><enddate>20100715</enddate><creator>Wang, Xinyi</creator><creator>Xu, Yao</creator><creator>Xu, Xiao</creator><creator>Hu, Ke</creator><creator>Xiang, Minghui</creator><creator>Li, Limei</creator><creator>Liu, Feng</creator><creator>Li, Na</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100715</creationdate><title>Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles</title><author>Wang, Xinyi ; Xu, Yao ; Xu, Xiao ; Hu, Ke ; Xiang, Minghui ; Li, Limei ; Liu, Feng ; Li, Na</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-be22e518ad62f26754a9e57acd46b321b3c6cb0cdd215a585d6f8bfbc13dff193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Gold - analysis</topic><topic>Gold - chemistry</topic><topic>Gold Nanoparticles</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Lysozyme</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microscopy, Electron, Transmission</topic><topic>Muramidase - urine</topic><topic>Plasmon Resonance Light-Scattering</topic><topic>Spectrometric and optical methods</topic><topic>Surface Plasmon Resonance - methods</topic><topic>Urinary sample</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xinyi</creatorcontrib><creatorcontrib>Xu, Yao</creatorcontrib><creatorcontrib>Xu, Xiao</creatorcontrib><creatorcontrib>Hu, Ke</creatorcontrib><creatorcontrib>Xiang, Minghui</creatorcontrib><creatorcontrib>Li, Limei</creatorcontrib><creatorcontrib>Liu, Feng</creatorcontrib><creatorcontrib>Li, Na</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xinyi</au><au>Xu, Yao</au><au>Xu, Xiao</au><au>Hu, Ke</au><au>Xiang, Minghui</au><au>Li, Limei</au><au>Liu, Feng</au><au>Li, Na</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2010-07-15</date><risdate>2010</risdate><volume>82</volume><issue>2</issue><spage>693</spage><epage>697</epage><pages>693-697</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4
nM citrate-capped AuNPs (13
nm in diameter), the linear range of the calibration curve was 15-50
nM with a detection limit of 13.1
nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20602956</pmid><doi>10.1016/j.talanta.2010.05.034</doi><tpages>5</tpages></addata></record> |
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| subjects | Analytical chemistry Chemistry Exact sciences and technology Gold - analysis Gold - chemistry Gold Nanoparticles Humans Limit of Detection Lysozyme Metal Nanoparticles - chemistry Microscopy, Electron, Transmission Muramidase - urine Plasmon Resonance Light-Scattering Spectrometric and optical methods Surface Plasmon Resonance - methods Urinary sample |
| title | Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles |
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