Anthocyanin stimulates in vitro development of cloned pig embryos by increasing the intracellular glutathione level and inhibiting reactive oxygen species

The objective was to examine the nuclear maturation of oocytes, embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT), and gene expression in SCNT embryos in pigs ( Sus scrofa) when anthocyanin was added to oocytes during maturation and in vitro culture...

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Veröffentlicht in:	Theriogenology 2010-09, Vol.74 (5), p.777-785
Hauptverfasser:	You, Jinyoung, Kim, Jinyoung, Lim, Jeongmook, Lee, Eunsong
Format:	Artikel
Sprache:	eng
Schlagworte:	additives Animals Anthocyanin anthocyanins Anthocyanins - pharmacology antioxidant activity blastocyst cloning (animals) Cloning, Organism - veterinary culture media developmental competence embryo (animal) embryo culture Embryo Culture Techniques - veterinary Embryo, Mammalian - drug effects embryogenesis Embryonic development Embryonic Development - drug effects glutathione Glutathione - metabolism in vitro culture nuclear reprogramming Nuclear Transfer Techniques - veterinary nuclear transplantation oocytes parthenogenesis Parthenogenesis - genetics parthenogenetic activation Pig Reactive oxygen species Reactive Oxygen Species - metabolism Somatic cell nuclear transfer somatic cells swine Swine - embryology transcription factors
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