A cautionary note on using N-acetylcysteine as an antagonist to assess isothiocyanate-induced reactive oxygen species-mediated apoptosis

N-Acetylcysteine (NAC) has been widely used in cell culture-based studies for the role of reactive oxygen species (ROS) generation in apoptosis induction by isothiocyanates (ITCs). Here we have demonstrated, using [ 14C]phenethyl ITC and [ 14C]sulforaphane, that NAC pretreatment significantly reduce...

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Veröffentlicht in:	Analytical biochemistry 2010-10, Vol.405 (2), p.269-271
Hauptverfasser:	Mi, Lixin, Sirajuddin, Paul, Gan, Nanqin, Wang, Xiantao
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcysteine - chemistry Apoptosis Carbon Isotopes - chemistry Cell Line, Tumor HeLa Cells Humans Isothiocyanates - antagonists & inhibitors Isothiocyanates - chemistry Reactive Oxygen Species - metabolism Thiocyanates - chemistry
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