A cautionary note on using N-acetylcysteine as an antagonist to assess isothiocyanate-induced reactive oxygen species-mediated apoptosis
N-Acetylcysteine (NAC) has been widely used in cell culture-based studies for the role of reactive oxygen species (ROS) generation in apoptosis induction by isothiocyanates (ITCs). Here we have demonstrated, using [ 14C]phenethyl ITC and [ 14C]sulforaphane, that NAC pretreatment significantly reduce...
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| Veröffentlicht in: | Analytical biochemistry 2010-10, Vol.405 (2), p.269-271 |
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| container_title | Analytical biochemistry |
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| creator | Mi, Lixin Sirajuddin, Paul Gan, Nanqin Wang, Xiantao |
| description | N-Acetylcysteine (NAC) has been widely used in cell culture-based studies for the role of reactive oxygen species (ROS) generation in apoptosis induction by isothiocyanates (ITCs). Here we have demonstrated, using [
14C]phenethyl ITC and [
14C]sulforaphane, that NAC pretreatment significantly reduces ITC cellular uptake by conjugating with ITCs in the medium, suggesting that reduced uptake of ITCs, rather than the antioxidant activity of NAC itself, is responsible for the diminished downstream apoptotic effects. The study provides a cautionary note on the assay in studying mechanisms of apoptosis by ITCs and other electrophilic and thiol-reactive compounds. |
| doi_str_mv | 10.1016/j.ab.2010.06.015 |
| format | Article |
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14C]phenethyl ITC and [
14C]sulforaphane, that NAC pretreatment significantly reduces ITC cellular uptake by conjugating with ITCs in the medium, suggesting that reduced uptake of ITCs, rather than the antioxidant activity of NAC itself, is responsible for the diminished downstream apoptotic effects. The study provides a cautionary note on the assay in studying mechanisms of apoptosis by ITCs and other electrophilic and thiol-reactive compounds.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2010.06.015</identifier><identifier>PMID: 20541518</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetylcysteine - chemistry ; Apoptosis ; Carbon Isotopes - chemistry ; Cell Line, Tumor ; HeLa Cells ; Humans ; Isothiocyanates - antagonists & inhibitors ; Isothiocyanates - chemistry ; Reactive Oxygen Species - metabolism ; Thiocyanates - chemistry</subject><ispartof>Analytical biochemistry, 2010-10, Vol.405 (2), p.269-271</ispartof><rights>2010 Elsevier Inc.</rights><rights>Copyright 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-d68b2fe07ef5dfa15a30833b6eac31d71629a9470342337093120ea4303895c93</citedby><cites>FETCH-LOGICAL-c457t-d68b2fe07ef5dfa15a30833b6eac31d71629a9470342337093120ea4303895c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2010.06.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20541518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mi, Lixin</creatorcontrib><creatorcontrib>Sirajuddin, Paul</creatorcontrib><creatorcontrib>Gan, Nanqin</creatorcontrib><creatorcontrib>Wang, Xiantao</creatorcontrib><title>A cautionary note on using N-acetylcysteine as an antagonist to assess isothiocyanate-induced reactive oxygen species-mediated apoptosis</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>N-Acetylcysteine (NAC) has been widely used in cell culture-based studies for the role of reactive oxygen species (ROS) generation in apoptosis induction by isothiocyanates (ITCs). Here we have demonstrated, using [
14C]phenethyl ITC and [
14C]sulforaphane, that NAC pretreatment significantly reduces ITC cellular uptake by conjugating with ITCs in the medium, suggesting that reduced uptake of ITCs, rather than the antioxidant activity of NAC itself, is responsible for the diminished downstream apoptotic effects. The study provides a cautionary note on the assay in studying mechanisms of apoptosis by ITCs and other electrophilic and thiol-reactive compounds.</description><subject>Acetylcysteine - chemistry</subject><subject>Apoptosis</subject><subject>Carbon Isotopes - chemistry</subject><subject>Cell Line, Tumor</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Isothiocyanates - antagonists & inhibitors</subject><subject>Isothiocyanates - chemistry</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Thiocyanates - chemistry</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhi1ERbeFOyfkG6cs4zhf5lZVpa1UwaWcrYkzWbzatUPGqcg_4Gfjags3JEsjj555R_MI8V7BVoFqPu232G9LyF9otqDqV2KjwDQFaDCvxQYAdFE2pj0XF8x7AKWqunkjzkuoK1WrbiN-X0mHS_Ix4LzKEBPJGOTCPuzk1wIdpfXgVk7kA0lkiSG_hLsYPCeZYu4xMUvPMf3w0a0YMFHhw7A4GuRM6JJ_yqG_1h0FyRM5T1wcafCZGyROcUqRPb8VZyMemN691Evx_cvN4_Vd8fDt9v766qFwVd2mYmi6vhwJWhrrYURVo4ZO677Ji7QaWtWUBk3Vgq5KrVswWpVAWGnQnamd0Zfi4yl3muPPhTjZo2dHhwMGigvbtupMWynTZRJOpJsj80yjnWZ_zJqsAvus3-4t9vZZv4XGZv155MNL-NLnE_8N_PWdgc8ngPKJT55my9lHyKr8TC7ZIfr_p_8BpMCWyw</recordid><startdate>20101015</startdate><enddate>20101015</enddate><creator>Mi, Lixin</creator><creator>Sirajuddin, Paul</creator><creator>Gan, Nanqin</creator><creator>Wang, Xiantao</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101015</creationdate><title>A cautionary note on using N-acetylcysteine as an antagonist to assess isothiocyanate-induced reactive oxygen species-mediated apoptosis</title><author>Mi, Lixin ; Sirajuddin, Paul ; Gan, Nanqin ; Wang, Xiantao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-d68b2fe07ef5dfa15a30833b6eac31d71629a9470342337093120ea4303895c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acetylcysteine - chemistry</topic><topic>Apoptosis</topic><topic>Carbon Isotopes - chemistry</topic><topic>Cell Line, Tumor</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Isothiocyanates - antagonists & inhibitors</topic><topic>Isothiocyanates - chemistry</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Thiocyanates - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mi, Lixin</creatorcontrib><creatorcontrib>Sirajuddin, Paul</creatorcontrib><creatorcontrib>Gan, Nanqin</creatorcontrib><creatorcontrib>Wang, Xiantao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mi, Lixin</au><au>Sirajuddin, Paul</au><au>Gan, Nanqin</au><au>Wang, Xiantao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A cautionary note on using N-acetylcysteine as an antagonist to assess isothiocyanate-induced reactive oxygen species-mediated apoptosis</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2010-10-15</date><risdate>2010</risdate><volume>405</volume><issue>2</issue><spage>269</spage><epage>271</epage><pages>269-271</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>N-Acetylcysteine (NAC) has been widely used in cell culture-based studies for the role of reactive oxygen species (ROS) generation in apoptosis induction by isothiocyanates (ITCs). Here we have demonstrated, using [
14C]phenethyl ITC and [
14C]sulforaphane, that NAC pretreatment significantly reduces ITC cellular uptake by conjugating with ITCs in the medium, suggesting that reduced uptake of ITCs, rather than the antioxidant activity of NAC itself, is responsible for the diminished downstream apoptotic effects. The study provides a cautionary note on the assay in studying mechanisms of apoptosis by ITCs and other electrophilic and thiol-reactive compounds.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>20541518</pmid><doi>10.1016/j.ab.2010.06.015</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 2010-10, Vol.405 (2), p.269-271 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acetylcysteine - chemistry Apoptosis Carbon Isotopes - chemistry Cell Line, Tumor HeLa Cells Humans Isothiocyanates - antagonists & inhibitors Isothiocyanates - chemistry Reactive Oxygen Species - metabolism Thiocyanates - chemistry |
| title | A cautionary note on using N-acetylcysteine as an antagonist to assess isothiocyanate-induced reactive oxygen species-mediated apoptosis |
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